FastQCFastQC Report
Fri 10 Feb 2017
ERR1630350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589011
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44830.28212517093965994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44730.28149584867568567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32760.206165973677967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.11994882351349362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1409.458745E-1118.55
TATAGCG500.00703603818.4999985
GGTATCA16550.018.2205451
AGTGCGC1351.1532393E-917.8148168
CTTATAC5850.016.76068537
TATTTCG957.062394E-515.5789485
CGCCAAT855.3657545E-415.23529414
TTATAGT2350.014.9574464
TAATACT2600.014.9423074
GTATAAA3250.014.81
GTGCGCA1508.106326E-814.89
ACCGTCG1405.9984995E-714.5357148
TAGGACG908.276473E-414.3888884
GTATAGG1551.210883E-714.322581
TCGACCG800.006300019613.8759
TTAGGAC6800.013.3308823
CGTTTAG1254.8466594E-513.32000126
CGAACGA850.009406798513.05882416
CTACCCG850.009406798513.0588244
ATACCGT1703.7332393E-713.0588246
Produced by FastQC (version 0.11.5)