##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630350.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589011
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473537313461016	31.0	31.0	34.0	30.0	34.0
2	31.70146336306042	31.0	31.0	34.0	30.0	34.0
3	31.820010056569778	31.0	31.0	34.0	30.0	34.0
4	35.544935182953424	37.0	35.0	37.0	33.0	37.0
5	35.414987057987645	37.0	35.0	37.0	33.0	37.0
6	35.47589790127318	37.0	35.0	37.0	33.0	37.0
7	35.464001822517275	37.0	35.0	37.0	33.0	37.0
8	35.46579161503602	37.0	35.0	37.0	33.0	37.0
9	37.15978744011212	39.0	37.0	39.0	33.0	39.0
10	36.99227066395387	39.0	37.0	39.0	33.0	39.0
11	37.092269342377115	39.0	37.0	39.0	33.0	39.0
12	36.99428638316538	39.0	37.0	39.0	33.0	39.0
13	37.01895959184675	39.0	37.0	39.0	33.0	39.0
14	38.160100842599576	40.0	38.0	41.0	33.0	41.0
15	38.156632018280554	40.0	38.0	41.0	33.0	41.0
16	38.099092454363124	40.0	37.0	41.0	33.0	41.0
17	38.06427331214196	40.0	37.0	41.0	33.0	41.0
18	38.011683367830685	40.0	37.0	41.0	33.0	41.0
19	38.06052884467131	40.0	37.0	41.0	33.0	41.0
20	38.030029999792326	40.0	37.0	41.0	33.0	41.0
21	37.964912137172114	40.0	37.0	41.0	33.0	41.0
22	37.92502569208143	40.0	37.0	41.0	33.0	41.0
23	37.86897825125188	40.0	37.0	41.0	33.0	41.0
24	37.831318977653396	40.0	37.0	41.0	33.0	41.0
25	37.809326052494285	40.0	37.0	41.0	33.0	41.0
26	37.61965209806603	39.0	37.0	41.0	32.0	41.0
27	37.49169514874346	39.0	37.0	41.0	32.0	41.0
28	37.41811919489544	39.0	37.0	41.0	32.0	41.0
29	37.35652301966443	39.0	36.0	41.0	32.0	41.0
30	37.28421326221153	39.0	36.0	40.0	31.0	41.0
31	37.22180148532641	39.0	36.0	40.0	31.0	41.0
32	37.1198229590607	39.0	36.0	40.0	31.0	41.0
33	37.025602717665265	39.0	36.0	40.0	31.0	41.0
34	36.957887642061635	39.0	36.0	40.0	31.0	41.0
35	36.82741088639411	39.0	36.0	40.0	30.0	41.0
36	36.72852359108905	39.0	35.0	40.0	30.0	41.0
37	36.66345544492769	39.0	35.0	40.0	30.0	41.0
38	36.55427810128438	39.0	35.0	40.0	30.0	41.0
39	36.46344738960271	39.0	35.0	40.0	30.0	41.0
40	36.35853559226462	39.0	35.0	40.0	30.0	41.0
41	36.24335325557847	39.0	35.0	40.0	30.0	41.0
42	36.20807344946007	38.0	35.0	40.0	29.0	41.0
43	34.95972715103923	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	4.0
12	3.0
13	1.0
14	6.0
15	4.0
16	30.0
17	43.0
18	89.0
19	201.0
20	391.0
21	706.0
22	1336.0
23	2285.0
24	3717.0
25	5674.0
26	8334.0
27	12185.0
28	17170.0
29	23304.0
30	30738.0
31	39854.0
32	50691.0
33	64937.0
34	84673.0
35	112994.0
36	157843.0
37	240748.0
38	419983.0
39	311053.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.59845337760405	20.073492253986913	12.774235043055082	25.55381932535395
2	18.25695353902522	21.863095976050513	34.950041252074406	24.929909232849866
3	19.78217897799323	23.706884345042294	30.435157465870283	26.075779211094197
4	14.902036549778447	16.272637508487982	34.973955498105425	33.85137044362814
5	14.250247480980308	37.07834621660895	34.48667126911016	14.184735033300589
6	33.864963804529985	35.836001135297366	15.952878866162665	14.346156194009986
7	28.42654959594364	31.32678124946901	21.69934632296441	18.54732283162294
8	26.649658183612324	34.66093060400463	20.232459057866812	18.456952154516237
9	26.418004658243397	14.54432977493548	20.017986030304385	39.01967953651674
10	16.37584635977976	27.565951399958845	33.79177362522978	22.266428615031614
11	34.956900864751724	22.264414783786897	21.961081452551305	20.817602898910074
12	20.670467353592894	26.16986288955835	30.069080704916455	23.0905890519323
13	29.628240459002487	20.27481244623228	25.59869000277531	24.498257091989924
14	22.28845489427071	21.321186574542278	25.734371882888162	30.65598664829885
15	24.687368432314187	28.372302016789057	22.888136079611783	24.05219347128497
16	24.272267466996766	27.248584182236623	24.434066221064548	24.04508212970206
17	22.911358071152435	27.282567584491236	26.122223194175497	23.683851150180836
18	22.75962847330824	26.06464020702185	27.339961774965687	23.83576954470422
19	23.653077291472492	26.298936885899472	27.369414056919684	22.678571765708355
20	24.17245695593045	25.66074117800317	27.173568968370894	22.993232897695485
21	23.899771618950403	26.272190689680563	26.365959707012728	23.46207798435631
22	23.87271076159951	25.940223195434143	26.639589027388734	23.547477015577613
23	23.351946588160814	26.210391243358288	26.733924434758478	23.70373773372242
24	23.9413698205991	26.252241173912576	26.63040092233471	23.17598808315361
25	24.00593828488286	25.783647816157345	26.680998432358237	23.529415466601552
26	23.787060001472614	26.191637439891856	26.560483218807168	23.460819339828358
27	23.82532279512225	25.95186565731766	26.355701754109944	23.86710979345014
28	23.360757099856453	26.214922363658903	26.852803410423213	23.57151712606143
29	23.231997764647318	26.509508115425255	26.861928583250837	23.396565536676587
30	23.162017128893382	27.079737018812335	26.684333840357304	23.073912011936983
31	23.724316571754382	26.41473218247073	26.630526786787506	23.230424458987383
32	22.95792791868653	26.589557907402785	26.785214199272378	23.66729997463831
33	23.148864293576317	26.199441035965137	27.03253784901426	23.61915682144428
34	23.567992921383173	26.017189308318194	26.90138708920203	23.513430681096605
35	23.611227360918207	26.09579165908858	27.093078650808582	23.19990232918463
36	23.23659181717433	26.432101476956422	26.743930658755666	23.58737604711358
37	23.811981163125996	25.49604754152111	26.95399843047027	23.73797286488262
38	23.29448946545996	25.9681021717282	27.373064126050732	23.364344236761106
39	23.19694451454395	25.74922388831795	27.51887809461357	23.534953502524523
40	22.946474253482197	25.42317202335289	28.07016439785502	23.560189325309892
41	22.474230826595914	25.724554455570164	28.224096623623122	23.577118094210803
42	23.001602883806342	25.278616699318	27.918497732237224	23.801282684638434
43	22.526024048920995	25.139473546753294	28.126551672707112	24.2079507316186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	273.0
1	251.5
2	230.0
3	486.5
4	743.0
5	743.0
6	1015.0
7	1287.0
8	1295.5
9	1304.0
10	1920.5
11	2537.0
12	2537.0
13	4287.0
14	6037.0
15	9066.0
16	12095.0
17	12026.0
18	11957.0
19	11957.0
20	14728.5
21	17500.0
22	18838.5
23	20177.0
24	23092.5
25	26008.0
26	26008.0
27	29701.5
28	33395.0
29	39812.5
30	46230.0
31	50551.0
32	54872.0
33	54872.0
34	60046.5
35	65221.0
36	70423.5
37	75626.0
38	82298.0
39	88970.0
40	88970.0
41	93890.5
42	98811.0
43	96613.5
44	94416.0
45	98084.5
46	101753.0
47	101753.0
48	105469.0
49	109185.0
50	111183.0
51	113181.0
52	116215.5
53	119250.0
54	119250.0
55	110374.0
56	101498.0
57	96484.0
58	91470.0
59	84083.5
60	76697.0
61	76697.0
62	70043.5
63	63390.0
64	56217.0
65	49044.0
66	42560.0
67	36076.0
68	36076.0
69	30676.0
70	25276.0
71	21811.5
72	18347.0
73	14856.5
74	11366.0
75	11366.0
76	8787.5
77	6209.0
78	5146.0
79	4083.0
80	3104.0
81	2125.0
82	2125.0
83	1552.0
84	979.0
85	798.5
86	618.0
87	517.0
88	416.0
89	416.0
90	350.5
91	285.0
92	166.0
93	47.0
94	32.5
95	18.0
96	18.0
97	11.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1589011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.5013635326938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.43954230108946	52.54648351126272
2	8.900432697572723	10.94777493863389
3	2.2979154783717948	4.239748056082431
4	1.002399630003034	2.465957761994174
5	0.5369428846638541	1.651135977300248
6	0.3297660285470839	1.2168636241444133
7	0.23081011619359323	0.9936595804131831
8	0.17890657271106256	0.8802398533353099
9	0.12279098349653893	0.679663162310188
>10	0.7890709702548148	9.220845173379558
>50	0.09097134059767906	3.9015771335573546
>100	0.07655170211248964	8.80952786890789
>500	0.0030783903047633303	1.2585708293684505
>1k	8.209040812702213E-4	1.1879525293101196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4483	0.28212517093965994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4473	0.28149584867568567	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3276	0.206165973677967	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1906	0.11994882351349362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.2586445279485163E-4	0.0
12	0.0	0.0	0.0	1.8879667919227745E-4	0.0
13	0.0	0.0	0.0	1.8879667919227745E-4	0.0
14	0.0	0.0	0.0	2.5172890558970327E-4	0.0
15	0.0	0.0	0.0	4.4052558478198075E-4	0.0
16	0.0	0.0	0.0	6.293222639742582E-4	0.0
17	0.0	0.0	0.0	9.439833959613873E-4	0.0
18	0.0	0.0	0.0	0.0011327800751536647	0.0
19	0.0	0.0	0.0	0.001447441207140794	0.0
20	0.0	0.0	0.0	0.001447441207140794	0.0
21	0.0	0.0	0.0	0.001762102339127923	0.0
22	0.0	0.0	0.0	0.0022655601503073293	0.0
23	0.0	0.0	0.0	0.0030207468670764392	0.0
24	0.0	0.0	0.0	0.00421645916862753	0.0
25	0.0	0.0	0.0	0.005097510338191492	0.0
26	0.0	0.0	0.0	0.007363070488498821	0.0
27	0.0	0.0	0.0	0.017495158938484377	0.0
28	0.0	0.0	0.0	0.04380082957260837	0.0
29	0.0	0.0	0.0	0.0787282152231797	0.0
30	0.0	0.0	0.0	0.12441701158771085	0.0
31	0.0	0.0	0.0	0.2611058073229197	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	140	9.458745E-11	18.5	5
TATAGCG	50	0.007036038	18.499998	5
GGTATCA	1655	0.0	18.220545	1
AGTGCGC	135	1.1532393E-9	17.814816	8
CTTATAC	585	0.0	16.760685	37
TATTTCG	95	7.062394E-5	15.578948	5
CGCCAAT	85	5.3657545E-4	15.235294	14
TTATAGT	235	0.0	14.957446	4
TAATACT	260	0.0	14.942307	4
GTATAAA	325	0.0	14.8	1
GTGCGCA	150	8.106326E-8	14.8	9
ACCGTCG	140	5.9984995E-7	14.535714	8
TAGGACG	90	8.276473E-4	14.388888	4
GTATAGG	155	1.210883E-7	14.32258	1
TCGACCG	80	0.0063000196	13.875	9
TTAGGAC	680	0.0	13.330882	3
CGTTTAG	125	4.8466594E-5	13.320001	26
CGAACGA	85	0.0094067985	13.058824	16
CTACCCG	85	0.0094067985	13.058824	4
ATACCGT	170	3.7332393E-7	13.058824	6
>>END_MODULE