FastQCFastQC Report
Fri 10 Feb 2017
ERR1630349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517072
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG17050.32974131262183987No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG12560.24290621035368382No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12090.2338165671318501No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT11030.2133165207166507No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC9260.17908531113655352No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG8360.16167961135006345No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA7990.15452393477117307No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC7930.15336355478540706No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC7530.1456276882136337No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7530.1456276882136337No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG7470.14446730822786769No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC7460.14427391156357336No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6960.13460407834885663No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6920.1338304916916793No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA6760.13073614506296996No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA6450.12474084846984558No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA6250.1208729151839589No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6160.1191323452053099No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT6090.11777856855524957No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5900.1141040319336572No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5890.11391063526936289No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC5880.11371723860506855No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA5870.11352384194077421No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT5540.10714175201906118No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG5450.10540118204041217No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC5450.10540118204041217No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5430.10501438871182349No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5190.10037286876875948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTA405.9339072E-527.752
ATACCAT358.8627683E-426.4285726
CTTATAC1051.8189894E-1224.66666837
TATAGCC451.3218368E-424.6666683
CTCTAAC400.001929758523.1251
TAGTACG400.001929758523.1254
GGTATCA2650.023.0377351
TTTTGTC502.699317E-422.226
AAGACGG957.1213435E-921.4210535
CGAGCCG806.9448834E-720.812515
TAAAACC450.003823003720.5555574
CTAACCA450.003823003720.5555579
AGGTTAT450.003823003720.5555571
ACGGACC909.452924E-820.5555578
GTATAGT450.003823003720.5555571
CAAGACG909.452924E-820.5555574
CGGGTTG656.892748E-519.92307714
TCTTATA2300.019.30434837
TAGAATA500.007029776518.54
GCGTTAT1002.8673276E-718.51
Produced by FastQC (version 0.11.5)