##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517072
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.676130210106137	31.0	30.0	33.0	27.0	34.0
2	30.942143453909708	31.0	31.0	34.0	27.0	34.0
3	31.022112974595416	31.0	31.0	34.0	27.0	34.0
4	34.900663737351856	35.0	35.0	37.0	32.0	37.0
5	34.36695276479871	35.0	35.0	37.0	30.0	37.0
6	34.481252127363305	35.0	35.0	37.0	30.0	37.0
7	34.5060301079927	35.0	35.0	37.0	30.0	37.0
8	34.47675952285175	35.0	35.0	37.0	30.0	37.0
9	36.114127239533374	37.0	35.0	39.0	32.0	39.0
10	35.775946096481725	37.0	35.0	39.0	30.0	39.0
11	35.95219621251972	37.0	35.0	39.0	31.0	39.0
12	35.79946699879321	37.0	35.0	39.0	30.0	39.0
13	35.853295092366245	37.0	35.0	39.0	30.0	39.0
14	36.77943884023888	38.0	36.0	40.0	31.0	41.0
15	36.73729770708915	38.0	36.0	40.0	31.0	41.0
16	36.644175279264786	38.0	36.0	40.0	31.0	41.0
17	36.58541170281895	38.0	36.0	40.0	31.0	41.0
18	36.47037549896339	38.0	35.0	40.0	31.0	41.0
19	36.52707553300121	38.0	35.0	40.0	30.0	41.0
20	36.48126566512981	38.0	35.0	40.0	30.0	41.0
21	36.382488318841474	38.0	35.0	40.0	30.0	41.0
22	36.33173523223071	38.0	35.0	40.0	30.0	41.0
23	36.25463185010985	38.0	35.0	40.0	30.0	41.0
24	36.18477890893338	38.0	35.0	40.0	30.0	41.0
25	36.13713950861776	38.0	34.0	40.0	30.0	41.0
26	35.880902466194264	38.0	34.0	40.0	29.0	41.0
27	35.749162592443604	38.0	34.0	40.0	29.0	41.0
28	35.630575625831604	38.0	34.0	40.0	27.0	41.0
29	35.551971485595814	38.0	34.0	40.0	27.0	41.0
30	35.47673051335211	38.0	34.0	40.0	27.0	41.0
31	35.40774012129839	38.0	34.0	40.0	27.0	41.0
32	35.27953940650432	38.0	34.0	40.0	27.0	41.0
33	35.14626976513909	38.0	34.0	40.0	27.0	41.0
34	35.073372760466626	38.0	33.0	40.0	26.0	40.0
35	34.92672393786552	38.0	33.0	40.0	26.0	40.0
36	34.790431506637375	38.0	33.0	40.0	25.0	40.0
37	34.768809759569265	37.0	33.0	40.0	25.0	40.0
38	34.61136360120061	37.0	33.0	40.0	25.0	40.0
39	34.50431661354705	37.0	33.0	39.0	25.0	40.0
40	34.39566249961321	37.0	33.0	39.0	24.0	40.0
41	34.27680090973791	37.0	33.0	39.0	24.0	40.0
42	34.24851471361822	37.0	33.0	39.0	24.0	40.0
43	32.829594331156976	36.0	30.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	0.0
13	2.0
14	3.0
15	12.0
16	33.0
17	54.0
18	121.0
19	212.0
20	396.0
21	624.0
22	1131.0
23	1701.0
24	2605.0
25	3832.0
26	5404.0
27	7560.0
28	9977.0
29	12956.0
30	16814.0
31	21338.0
32	27574.0
33	34893.0
34	45326.0
35	59882.0
36	77843.0
37	91503.0
38	74528.0
39	20744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43189343070211	20.26661664139617	13.498893771080237	25.80259615682149
2	19.474270507782283	22.265757960206702	33.68931212674443	24.57065940526658
3	20.640258996812825	23.335048117090075	30.423809450134602	25.600883435962494
4	16.63404709595569	16.3230652597704	32.896192406473375	34.14669523780054
5	14.574952811213912	37.70635424080206	33.5303400686945	14.188352879289537
6	35.32428752668874	35.053918990005265	15.112595537952162	14.509197945353838
7	29.228424668131325	30.974022960051983	20.787433858340812	19.01011851347588
8	26.36518705325371	34.93826778475725	19.946351765324753	18.750193396664294
9	26.427073985827892	14.803354271745519	18.666839434353435	40.10273230807315
10	16.770971934276076	27.926671720766162	32.3455921032274	22.956764241730358
11	36.3599266639849	21.72753504347557	21.01100040226506	20.90153789027447
12	21.567402605439863	25.78538385369929	28.016601169663026	24.63061237119782
13	30.112827613949317	19.083609245907727	25.33148188260049	25.472081257542474
14	23.122118389702013	20.76248568864684	23.493439985147134	32.621955936504
15	25.736454497632828	28.401460531608752	21.418873967261813	24.44321100349661
16	26.149356375901228	26.41759754927747	22.458767831172448	24.974278243648854
17	24.811244855648727	26.78195686480799	23.624949716867285	24.78184856267599
18	23.528638178048705	25.07233035244608	25.995606027787233	25.40342544171798
19	25.658902435250795	25.450034037812912	25.143693721570692	23.747369805365597
20	25.348113995729804	24.410333570566575	25.56877958968964	24.672772844013988
21	25.295703499706036	25.82406318655816	23.82376148776186	25.056471825973947
22	25.3241328093573	24.915679054367672	24.261418139059938	25.49876999721509
23	24.075177151344494	25.492001113964786	24.832325092056813	25.600496642633907
24	26.136205402729214	25.70899217130303	24.15988489030541	23.994917535662346
25	25.646331652071662	24.828070365442336	24.846636445214592	24.678961537271405
26	25.935072871863106	25.39452919516044	23.886808800321813	24.78358913265464
27	25.667798681808335	24.570079215273697	24.310154098462107	25.45196800445586
28	24.474154469783706	25.138665408299037	25.2593449268187	25.12783519509855
29	24.428512857010244	25.724850697775164	24.705650276944024	25.14098616827057
30	24.017544945384785	26.8945137234273	24.433927963610483	24.654013367577434
31	25.607652319212796	25.18063248445091	24.607017978153912	24.60469721818238
32	24.09026209115945	25.568199399696756	24.71299935018721	25.62853915895659
33	24.68689080050747	24.87990067147322	24.87100442491568	25.562204103103632
34	25.196684407587338	24.838320388649937	24.95938670049819	25.005608503264536
35	25.517722870315936	24.738527709874063	24.94062722406164	24.80312219574837
36	24.534881022372126	25.844950026301944	24.84238171860012	24.77778723272581
37	26.24121979144104	24.0680214747656	24.54841878887273	25.14233994492063
38	24.811438252313025	24.705843673608317	25.952285174985303	24.530432899093356
39	25.12009932852678	24.12313952408949	25.876086889253337	24.880674258130394
40	24.442824210168023	23.821827521118916	26.708079339047558	25.027268929665503
41	23.736346195500822	24.545131045579726	27.097386824272057	24.6211359346474
42	25.105207785376116	23.544690101185132	26.395743726212213	24.954358387226538
43	23.838266237583934	23.527864591391527	26.988117708945758	25.64575146207878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	5.0
2	4.0
3	18.0
4	32.0
5	32.0
6	56.5
7	81.0
8	78.5
9	76.0
10	113.5
11	151.0
12	151.0
13	272.5
14	394.0
15	783.5
16	1173.0
17	1164.5
18	1156.0
19	1156.0
20	1390.0
21	1624.0
22	2101.5
23	2579.0
24	3202.0
25	3825.0
26	3825.0
27	4503.0
28	5181.0
29	8026.5
30	10872.0
31	11450.5
32	12029.0
33	12029.0
34	13854.0
35	15679.0
36	17236.0
37	18793.0
38	23510.5
39	28228.0
40	28228.0
41	31390.0
42	34552.0
43	32429.0
44	30306.0
45	33851.0
46	37396.0
47	37396.0
48	40461.5
49	43527.0
50	45243.5
51	46960.0
52	50591.0
53	54222.0
54	54222.0
55	46081.0
56	37940.0
57	34852.0
58	31764.0
59	28676.5
60	25589.0
61	25589.0
62	23709.5
63	21830.0
64	19298.0
65	16766.0
66	14495.0
67	12224.0
68	12224.0
69	10138.5
70	8053.0
71	7068.5
72	6084.0
73	4810.5
74	3537.0
75	3537.0
76	2676.5
77	1816.0
78	1527.5
79	1239.0
80	942.0
81	645.0
82	645.0
83	469.5
84	294.0
85	241.5
86	189.0
87	161.0
88	133.0
89	133.0
90	115.5
91	98.0
92	58.5
93	19.0
94	11.0
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	517072.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.32306449386963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.92994983267049	46.007644360375394
2	6.750897828213713	7.064553249143011
3	1.8237386806023714	2.8627078981536775
4	0.8413455031027026	1.760871000818796
5	0.452556905429417	1.1839582074964723
6	0.33063015803361345	1.037974984944664
7	0.23073700850227102	0.845100716389081
8	0.1520013880975579	0.6362542746068984
9	0.13157493038932014	0.6195963211683124
>10	1.0035568497937553	10.890071994106032
>50	0.17465140867065526	6.4319252904106765
>100	0.16760602273466804	16.438263406004246
>500	0.009270244620280312	3.198015883401216
>1k	0.00148323913924485	1.023062412981533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1705	0.32974131262183987	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1256	0.24290621035368382	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1209	0.2338165671318501	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1103	0.2133165207166507	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	926	0.17908531113655352	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	836	0.16167961135006345	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	799	0.15452393477117307	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	793	0.15336355478540706	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	753	0.1456276882136337	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	753	0.1456276882136337	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	747	0.14446730822786769	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	746	0.14427391156357336	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	696	0.13460407834885663	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	692	0.1338304916916793	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	676	0.13073614506296996	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	645	0.12474084846984558	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	625	0.1208729151839589	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	616	0.1191323452053099	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	609	0.11777856855524957	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	590	0.1141040319336572	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	589	0.11391063526936289	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	588	0.11371723860506855	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	587	0.11352384194077421	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	554	0.10714175201906118	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	545	0.10540118204041217	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	545	0.10540118204041217	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	543	0.10501438871182349	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	519	0.10037286876875948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9339666429433425E-4	0.0
5	0.0	0.0	0.0	1.9339666429433425E-4	0.0
6	0.0	0.0	0.0	1.9339666429433425E-4	0.0
7	0.0	0.0	0.0	1.9339666429433425E-4	0.0
8	0.0	0.0	0.0	1.9339666429433425E-4	0.0
9	0.0	0.0	0.0	1.9339666429433425E-4	0.0
10	0.0	0.0	0.0	1.9339666429433425E-4	0.0
11	0.0	0.0	0.0	1.9339666429433425E-4	0.0
12	0.0	0.0	0.0	1.9339666429433425E-4	0.0
13	0.0	0.0	0.0	3.867933285886685E-4	0.0
14	0.0	0.0	0.0	3.867933285886685E-4	0.0
15	0.0	0.0	0.0	3.867933285886685E-4	0.0
16	0.0	0.0	0.0	7.73586657177337E-4	0.0
17	0.0	0.0	0.0	9.669833214716713E-4	0.0
18	0.0	0.0	0.0	0.0013537766500603397	0.0
19	0.0	0.0	0.0	0.0013537766500603397	0.0
20	0.0	0.0	0.0	0.0013537766500603397	0.0
21	0.0	0.0	0.0	0.0017405699786490082	0.0
22	0.0	0.0	0.0	0.0017405699786490082	0.0
23	0.0	0.0	0.0	0.002127363307237677	0.0
24	0.0	0.0	0.0	0.002320759971532011	0.0
25	0.0	0.0	0.0	0.002514156635826345	0.0
26	0.0	0.0	0.0	0.003867933285886685	0.0
27	0.0	0.0	0.0	0.006962279914596033	0.0
28	0.0	0.0	0.0	0.020693443079493765	0.0
29	0.0	0.0	0.0	0.04409443945910821	0.0
30	0.0	0.0	0.0	0.07890583903208838	0.0
31	0.0	0.0	0.0	0.20538725748058298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGTA	40	5.9339072E-5	27.75	2
ATACCAT	35	8.8627683E-4	26.428572	6
CTTATAC	105	1.8189894E-12	24.666668	37
TATAGCC	45	1.3218368E-4	24.666668	3
CTCTAAC	40	0.0019297585	23.125	1
TAGTACG	40	0.0019297585	23.125	4
GGTATCA	265	0.0	23.037735	1
TTTTGTC	50	2.699317E-4	22.2	26
AAGACGG	95	7.1213435E-9	21.421053	5
CGAGCCG	80	6.9448834E-7	20.8125	15
TAAAACC	45	0.0038230037	20.555557	4
CTAACCA	45	0.0038230037	20.555557	9
AGGTTAT	45	0.0038230037	20.555557	1
ACGGACC	90	9.452924E-8	20.555557	8
GTATAGT	45	0.0038230037	20.555557	1
CAAGACG	90	9.452924E-8	20.555557	4
CGGGTTG	65	6.892748E-5	19.923077	14
TCTTATA	230	0.0	19.304348	37
TAGAATA	50	0.0070297765	18.5	4
GCGTTAT	100	2.8673276E-7	18.5	1
>>END_MODULE