FastQCFastQC Report
Fri 10 Feb 2017
ERR1630348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029213
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC60760.2994264278811539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.26295908807996005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52220.2573411465430194No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT50060.24669662573618442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40130.19776139813809593No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA40050.19736715662673165No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG38990.19214345660115523No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA37180.18322374240653888No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG33960.1673555215741275No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG32030.1578444451124648No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG31900.15720380265649786No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG30760.15158586111955719No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG30690.15124089979711347No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA29080.14330678938090777No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG28270.13931509407834464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.1213278251223504No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA24220.1193566175655291No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC23690.11674476755274088No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC22460.11068330431551542No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT22200.10940201940358159No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA22120.10900777789221733No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA22010.10846569581409148No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA20540.10122150804277323No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG20460.10082726653140898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTAT11350.019.233481
CTAGTAC902.153869E-618.53
CGAATTA1601.8189894E-1218.515
GGTATCA21650.018.3718241
CGAGCGT752.068249E-417.2666666
CTTTATT13900.016.237412
TATTGAG13150.015.8973395
GTTCGCG700.002593410215.85714330
TACGACG1751.3278623E-1015.8571435
TTACTCG1059.350071E-615.85714219
CCGAATT2055.456968E-1215.34146314
TTTATTG15100.015.31456853
TATAACG855.3666654E-415.2352942
GTATAGA2950.015.0508491
CTTATAC8000.014.5687537
TCTAGCG1152.2113816E-514.47826128
TTATTGA15100.014.3344364
ATAACGC2600.014.2307683
TCGTTTA1854.9039954E-914.030
CGACGGT1854.9039954E-914.07
Produced by FastQC (version 0.11.5)