FastQCFastQC Report
Fri 10 Feb 2017
ERR1630347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100281
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1130.11268335975907699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAA400.001918983523.1250021
CGGACCA400.001918983523.1250029
GATTAAG450.003801863220.55555521
CGGGGCT609.156897E-418.512
ACGCGCG609.156897E-418.521
CTAAGGG500.00699127518.4999984
CAACTTC500.00699127518.49999824
CGCTACA752.043654E-417.26666626
CCTTAGA650.001566376217.0769231
CGCGCTA650.001566376217.07692324
CGCGCGC650.001566376217.07692322
GCACGCG650.001566376217.07692319
CTTAGAT650.001566376217.0769232
TATCAAC700.0025704315.8571421
GCGCGCT700.0025704315.85714223
CACGCGC700.0025704315.85714220
ATGTCCG700.0025704315.8571427
ATCAACG700.0025704315.8571422
TAGATGT700.0025704315.8571424
TCAACGC700.0025704315.8571423
Produced by FastQC (version 0.11.5)