##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100281
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.20395688116393	31.0	30.0	31.0	27.0	34.0
2	30.464245470228658	31.0	30.0	31.0	27.0	34.0
3	30.552218266670657	31.0	30.0	33.0	27.0	34.0
4	34.403775391150866	35.0	35.0	37.0	30.0	37.0
5	33.50730447442686	35.0	33.0	37.0	28.0	37.0
6	33.67395618312542	35.0	33.0	37.0	29.0	37.0
7	33.71138101933567	35.0	33.0	37.0	29.0	37.0
8	33.64558590361085	35.0	33.0	37.0	29.0	37.0
9	35.15776667564145	37.0	34.0	39.0	30.0	39.0
10	34.73824553006053	37.0	33.0	39.0	28.0	39.0
11	35.022038073014826	37.0	34.0	39.0	29.0	39.0
12	34.76016393933048	37.0	33.0	39.0	28.0	39.0
13	34.915397732372035	37.0	33.0	39.0	29.0	39.0
14	35.69736041722759	38.0	34.0	40.0	29.0	40.0
15	35.606246447482576	38.0	34.0	40.0	29.0	40.0
16	35.53548528634537	38.0	33.0	40.0	28.0	40.0
17	35.425923155931834	38.0	33.0	39.0	27.0	40.0
18	35.33420089548369	37.0	33.0	39.0	27.0	40.0
19	35.33719248910561	37.0	34.0	39.0	27.0	40.0
20	35.25790528614593	37.0	33.0	39.0	27.0	40.0
21	35.12600592335537	37.0	33.0	39.0	27.0	40.0
22	35.07623577746532	37.0	33.0	39.0	27.0	40.0
23	34.933606565550804	37.0	33.0	39.0	27.0	40.0
24	34.88696762098503	37.0	33.0	39.0	27.0	40.0
25	34.816056880166734	37.0	33.0	39.0	27.0	40.0
26	34.55205871501082	37.0	33.0	39.0	26.0	40.0
27	34.34639662548239	37.0	32.0	39.0	25.0	40.0
28	34.19119274837706	37.0	32.0	39.0	25.0	40.0
29	34.03504153329145	37.0	32.0	39.0	24.0	40.0
30	33.90203528086078	37.0	32.0	39.0	24.0	40.0
31	33.801248491738214	36.0	31.0	39.0	24.0	40.0
32	33.64103868130553	36.0	31.0	39.0	24.0	40.0
33	33.55864022097905	36.0	31.0	39.0	24.0	40.0
34	33.36674943409021	36.0	31.0	39.0	23.0	40.0
35	33.25062574166592	36.0	31.0	39.0	23.0	40.0
36	33.09664841794557	36.0	30.0	39.0	22.0	40.0
37	32.98584976216831	36.0	30.0	39.0	22.0	40.0
38	32.83139378346846	36.0	30.0	38.0	21.0	40.0
39	32.69259381139	35.0	30.0	38.0	21.0	40.0
40	32.51384609248013	35.0	30.0	38.0	20.0	40.0
41	32.36473509438478	35.0	30.0	38.0	19.0	40.0
42	32.1973753751957	35.0	30.0	38.0	19.0	40.0
43	30.861140196049103	34.0	27.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	1.0
15	9.0
16	16.0
17	34.0
18	50.0
19	95.0
20	186.0
21	254.0
22	440.0
23	658.0
24	1030.0
25	1368.0
26	1873.0
27	2446.0
28	3172.0
29	4016.0
30	5020.0
31	6044.0
32	7432.0
33	9032.0
34	10542.0
35	12381.0
36	12838.0
37	11201.0
38	7583.0
39	2553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.808328596643435	20.294971131121546	12.983516319143206	25.91318395309181
2	22.546643930555142	21.611272324767402	29.996709247015886	25.84537449766157
3	22.29136127481776	22.28637528544789	28.19776428236655	27.2244991573678
4	17.99343843798925	15.833507842961279	31.92528993528186	34.24776378376762
5	16.74793829339556	35.135269891604594	32.709087464225526	15.407704350774324
6	37.899502398260886	33.807002323471046	13.829140116273273	14.464355161994794
7	31.461592923883885	28.978570217688297	19.748506696183725	19.811330162244094
8	28.63553414904119	31.69892601788973	18.78022756055484	20.885312272514234
9	27.547591268535417	13.379403875110937	18.46212143875709	40.610883417596554
10	18.233763125617017	25.380680288389623	31.54635474317169	24.83920184282167
11	39.81711390991315	19.850220879329086	19.092350495108747	21.240314715649024
12	23.27060958706036	24.003550024431348	27.09685782949911	25.628982559009184
13	32.51961986817044	18.194872408532024	22.751069494719836	26.534438228577695
14	24.353566478196267	19.60989619170132	22.570576679530518	33.465960650571894
15	27.67822419002603	26.545407405191412	19.680697240753485	26.095671164029078
16	27.583490391998485	24.702585734087215	21.427787915956163	26.28613595795814
17	26.260208813234808	24.453286265593682	22.929567914161204	26.356937007010302
18	26.263200406856736	23.257646014698697	24.104267009702735	26.374886568741836
19	26.601250486133964	23.744278577198074	23.218755297613704	26.43571563905426
20	27.345160100118665	23.110060729350526	23.137982269821798	26.406796900709008
21	27.877663764820852	23.515920264057996	22.706195590391	25.90022038073015
22	27.65927743042052	23.58871570885811	22.7371087244842	26.014898136237175
23	26.990157656983875	22.987405390851706	23.228727276353446	26.793709675810973
24	26.70495906502727	24.15911289277131	22.853780875739176	26.282147166462238
25	27.206549595636265	23.237702057219213	22.976436214237992	26.579312132906534
26	27.149709316819738	24.058395907499925	22.899651977941986	25.89224279773836
27	27.285328227680218	23.21975249548768	23.08911957399707	26.405799702835033
28	26.939300565411195	23.191830955016403	23.357365802096112	26.51150267747629
29	26.128578693870224	23.72134302609667	23.663505549406167	26.48657273062694
30	26.21932370040187	24.224927952453605	23.414206080912635	26.141542266231887
31	27.411972357674934	23.869925509318815	22.53168596244553	26.186416170560722
32	26.51150267747629	23.684446704759626	22.59251503275795	27.211535585006132
33	26.15450583859355	23.50195949382236	23.5119314725621	26.831603195021987
34	27.293305810672013	23.329444261624836	22.946520278018767	26.430729649684388
35	27.175636461543064	23.363348989339954	23.592704500354007	25.86831004876298
36	26.660085160698436	23.631595217438996	23.310497502019327	26.39782211984324
37	26.910381827065944	23.095102761240913	23.64655318554861	26.347962226144535
38	26.29211914520198	23.402239706424947	24.32265334410307	25.982987804270003
39	26.488567126374885	22.800929388418545	24.149140914031573	26.561362571174996
40	26.133564683240095	23.033276493054515	24.869117779040895	25.964041044664494
41	25.896231589234254	22.690240424407413	25.307884843589513	26.10564314276882
42	26.2412620536293	22.760044275585606	25.20517346257018	25.793520208214915
43	25.57014788444471	21.913423280581565	25.257027752016832	27.25940108295689
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	3.5
4	7.0
5	7.0
6	8.0
7	9.0
8	9.5
9	10.0
10	16.5
11	23.0
12	23.0
13	34.0
14	45.0
15	67.0
16	89.0
17	97.5
18	106.0
19	106.0
20	152.0
21	198.0
22	222.0
23	246.0
24	302.5
25	359.0
26	359.0
27	484.0
28	609.0
29	769.5
30	930.0
31	1184.5
32	1439.0
33	1439.0
34	1819.0
35	2199.0
36	2564.0
37	2929.0
38	3425.5
39	3922.0
40	3922.0
41	4385.0
42	4848.0
43	5234.0
44	5620.0
45	6107.5
46	6595.0
47	6595.0
48	7007.0
49	7419.0
50	7706.5
51	7994.0
52	8343.0
53	8692.0
54	8692.0
55	8579.0
56	8466.0
57	8244.0
58	8022.0
59	7839.5
60	7657.0
61	7657.0
62	7096.5
63	6536.0
64	5837.0
65	5138.0
66	4413.0
67	3688.0
68	3688.0
69	3093.5
70	2499.0
71	2131.5
72	1764.0
73	1383.0
74	1002.0
75	1002.0
76	780.5
77	559.0
78	434.5
79	310.0
80	243.5
81	177.0
82	177.0
83	125.0
84	73.0
85	60.0
86	47.0
87	35.0
88	23.0
89	23.0
90	23.0
91	23.0
92	13.5
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	100281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.10509468393813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.66595354898996	75.15680936568243
2	4.16682035582113	6.759007189796671
3	1.126234123910344	2.7402997576809165
4	0.5815597604908217	1.8866983775590591
5	0.33688662658453516	1.3661610873445618
6	0.2163943294849569	1.0530409549166841
7	0.17090233976368754	0.970273531376831
8	0.12172181033528826	0.7897807161875131
9	0.10327911179963851	0.7538815927244443
>10	0.48319870163402306	6.99733748167649
>50	0.02581977794990963	1.4140265852953202
>100	0.0012295132357099824	0.11268335975907699
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	113	0.11268335975907699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	9.971978739741327E-4	0.0
23	0.0	0.0	0.0	9.971978739741327E-4	0.0
24	0.0	0.0	0.0	0.003988791495896531	0.0
25	0.0	0.0	0.0	0.004985989369870663	0.0
26	0.0	0.0	0.0	0.005983187243844796	0.0
27	0.0	0.0	0.0	0.012963572361663725	0.0
28	0.0	0.0	0.0	0.02991593621922398	0.0
29	0.0	0.0	0.0	0.05384868519460317	0.0
30	0.0	0.0	0.0	0.09373660015356847	0.0
31	0.0	0.0	0.0	0.18847039818111108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCTAA	40	0.0019189835	23.125002	1
CGGACCA	40	0.0019189835	23.125002	9
GATTAAG	45	0.0038018632	20.555555	21
CGGGGCT	60	9.156897E-4	18.5	12
ACGCGCG	60	9.156897E-4	18.5	21
CTAAGGG	50	0.006991275	18.499998	4
CAACTTC	50	0.006991275	18.499998	24
CGCTACA	75	2.043654E-4	17.266666	26
CCTTAGA	65	0.0015663762	17.076923	1
CGCGCTA	65	0.0015663762	17.076923	24
CGCGCGC	65	0.0015663762	17.076923	22
GCACGCG	65	0.0015663762	17.076923	19
CTTAGAT	65	0.0015663762	17.076923	2
TATCAAC	70	0.00257043	15.857142	1
GCGCGCT	70	0.00257043	15.857142	23
CACGCGC	70	0.00257043	15.857142	20
ATGTCCG	70	0.00257043	15.857142	7
ATCAACG	70	0.00257043	15.857142	2
TAGATGT	70	0.00257043	15.857142	4
TCAACGC	70	0.00257043	15.857142	3
>>END_MODULE