FastQCFastQC Report
Fri 10 Feb 2017
ERR1630346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275347
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.41947070423865157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10340.3755261542707929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7280.2643936560049683No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5720.2077378725753322No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG5410.19647935150918658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.11076932016691665No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA2880.10459529248548197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCG250.005489174329.637
AACGCCG405.9239843E-527.755
GGTATCA3800.027.2631591
TAACGCC358.8522E-426.4285724
CGCCGCA509.058336E-625.90000210
TATACTG451.3196444E-424.6666665
CGAACGA551.8958533E-523.54545416
ATAACGC400.001927479423.1253
AATAACG400.001927479423.1252
CGCATCG502.694875E-422.213
CCGGTCG502.694875E-422.220
ATCGCCG502.694875E-422.216
TAACGAA603.7130518E-521.58333413
GCGCAAG1550.021.483871
CGCAAGA1550.021.483872
ACACAGT450.00381853220.5555556
TATTCCC909.4127245E-820.5555555
TCGCCGG555.129452E-420.18181817
CTGAGTA555.129452E-420.1818189
CGGCATC555.129452E-420.18181825
Produced by FastQC (version 0.11.5)