##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630346.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275347
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.246779518207934	31.0	28.0	31.0	25.0	34.0
2	29.58573726969969	31.0	30.0	31.0	26.0	34.0
3	29.66103498494627	31.0	30.0	31.0	26.0	34.0
4	33.25009170246997	35.0	32.0	37.0	28.0	37.0
5	31.550483571638697	35.0	30.0	35.0	22.0	37.0
6	32.0069512288131	35.0	31.0	35.0	25.0	37.0
7	32.19422038373398	35.0	32.0	35.0	25.0	37.0
8	32.12443934380981	35.0	32.0	35.0	25.0	37.0
9	33.407987012751185	35.0	32.0	38.0	25.0	39.0
10	32.84077908965778	35.0	30.0	37.0	23.0	39.0
11	33.28585384986944	35.0	32.0	38.0	25.0	39.0
12	32.981125634199756	35.0	31.0	37.0	25.0	39.0
13	33.218193770042895	35.0	32.0	38.0	25.0	39.0
14	33.731880136700234	36.0	32.0	39.0	25.0	40.0
15	33.70342876443179	36.0	31.0	39.0	25.0	40.0
16	33.63341529052432	36.0	31.0	39.0	25.0	40.0
17	33.53138040363614	36.0	31.0	38.0	24.0	40.0
18	33.46239472374858	36.0	31.0	38.0	24.0	40.0
19	33.46119260424119	36.0	31.0	39.0	24.0	40.0
20	33.35801370634145	36.0	31.0	39.0	24.0	40.0
21	33.28253077026443	36.0	31.0	39.0	23.0	40.0
22	33.18635394611163	36.0	31.0	39.0	23.0	40.0
23	33.04535731277261	36.0	30.0	39.0	22.0	40.0
24	32.94632227698141	36.0	30.0	39.0	21.0	40.0
25	32.872470010568485	36.0	30.0	39.0	21.0	40.0
26	32.55578597188275	35.0	30.0	38.0	19.0	40.0
27	32.394251617050486	35.0	30.0	38.0	18.0	40.0
28	32.16984386973528	35.0	30.0	38.0	18.0	40.0
29	31.97759191129738	35.0	29.0	38.0	18.0	40.0
30	31.820383007623107	35.0	29.0	38.0	17.0	40.0
31	31.663671657944338	35.0	28.0	38.0	17.0	40.0
32	31.43927843775309	34.0	28.0	38.0	16.0	40.0
33	31.32000711829074	34.0	27.0	38.0	16.0	40.0
34	31.15524774194017	34.0	27.0	38.0	16.0	40.0
35	30.992711015554917	34.0	27.0	38.0	15.0	40.0
36	30.833635376452257	34.0	27.0	38.0	15.0	40.0
37	30.740803422590403	34.0	26.0	38.0	15.0	40.0
38	30.536722753471075	34.0	26.0	38.0	15.0	40.0
39	30.337810108699205	33.0	26.0	38.0	14.0	40.0
40	30.168834234620316	33.0	25.0	38.0	13.0	40.0
41	29.926336586198506	33.0	25.0	38.0	13.0	40.0
42	29.795098548377137	33.0	25.0	38.0	12.0	40.0
43	28.605356150602695	32.0	23.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	13.0
12	22.0
13	50.0
14	107.0
15	151.0
16	287.0
17	476.0
18	810.0
19	1293.0
20	1917.0
21	2795.0
22	3816.0
23	5160.0
24	6750.0
25	8467.0
26	10630.0
27	12780.0
28	14758.0
29	17085.0
30	19001.0
31	20517.0
32	22015.0
33	22283.0
34	22075.0
35	20849.0
36	19501.0
37	17778.0
38	15949.0
39	8009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00351193221644	23.354530828372926	14.248566354454562	23.393390884956073
2	21.593843404867314	24.836297471917256	30.826557035304543	22.74330208791089
3	23.25029871398635	24.393220191249586	29.61354218495208	22.742938909811983
4	19.55132977660915	17.494289024394675	32.17104235746168	30.7833388415345
5	14.982912470446383	38.61127958539588	32.66169596908628	13.744111975071455
6	33.949525507813775	36.52845318815894	14.960032250215182	14.5619890538121
7	29.52056859163165	31.99381144519461	20.399350637559152	18.086269325614587
8	28.573400109679785	33.80643333684405	19.563677831972022	18.056488721504138
9	27.95853958822867	13.968192862097645	19.51138018572928	38.5618873639444
10	18.014360062030818	27.751528071851155	31.587051974417733	22.64705989170029
11	37.23301870004031	21.976633121116265	20.068132211355127	20.722215967488296
12	21.74274642541956	27.019724202551686	28.260703766520063	22.976825605508687
13	31.59649460498934	19.369014370957373	24.953603997864512	24.080887026188773
14	23.788528656567895	20.825721725677056	25.005538466008346	30.380211151746707
15	26.501105877311176	28.192426283925375	21.66248406556091	23.64398377320254
16	25.476943638390832	26.798548740316765	23.442056750209737	24.28245087108267
17	23.907651073009692	27.046599381870877	24.8980377487316	24.14771179638783
18	24.14589590589329	24.694294835244254	26.86718940101036	24.29261985785209
19	24.785452538070142	25.48892851565479	26.46696713601383	23.258651810261235
20	25.89132984924477	23.952685157274274	26.336586198505884	23.81939879497507
21	26.53815004339978	24.953240819765604	25.07817408578993	23.430435051044682
22	25.93890618020171	25.15371513036278	25.569190875513442	23.33818781392207
23	25.250683682771196	25.05928882464672	25.879708150079715	23.81031934250237
24	25.08180586677901	25.495465721435135	26.038416979302482	23.384311432483376
25	25.43844675990659	24.553381732867983	25.786008200561472	24.222163306663955
26	24.442249234602155	25.950527879366764	25.791819050144	23.815403835887082
27	25.674149346097835	24.60095806382492	25.81978376375991	23.90510882631734
28	25.248141436078843	24.877699775192756	26.36055595303381	23.5136028356946
29	24.571903815912286	25.070910523811772	26.68668988585312	23.67049577442282
30	24.773830838905088	25.78673455675929	26.224727344042243	23.214707260293373
31	25.39268631944419	25.679233839482546	25.63093115232779	23.297148688745473
32	24.805064155411173	25.568827697414537	25.521614544556503	24.104493602617787
33	24.58388869317625	25.454789774357444	26.208747507690294	23.75257402477601
34	25.38978089465293	24.612942941088882	26.309347841087792	23.6879283231704
35	25.220176722462927	24.654708422463294	26.96343159722096	23.16168325785282
36	24.411015918096073	25.713735758878798	26.03151659542323	23.8437317276019
37	25.827773681935884	24.994643123041108	25.91275735708034	23.264825837942666
38	24.944161367292907	25.365084783927188	26.060207665236955	23.630546183542947
39	25.486386268962434	24.728070398442693	25.931279440124644	23.85426389247023
40	24.368524080523848	24.559555760549415	27.036793573200363	24.035126585726374
41	24.15788078315725	24.94525090158963	27.23799423999535	23.658874075257767
42	25.027329151942823	24.390314766458324	26.818160357657796	23.764195723941064
43	24.70046886292569	23.776543779303932	26.814165398569806	24.708821959200574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	31.5
2	34.0
3	59.5
4	85.0
5	85.0
6	122.0
7	159.0
8	165.0
9	171.0
10	255.5
11	340.0
12	340.0
13	604.5
14	869.0
15	1553.5
16	2238.0
17	2076.5
18	1915.0
19	1915.0
20	2189.0
21	2463.0
22	2382.5
23	2302.0
24	2647.5
25	2993.0
26	2993.0
27	3521.0
28	4049.0
29	5096.5
30	6144.0
31	7083.5
32	8023.0
33	8023.0
34	9036.0
35	10049.0
36	10904.5
37	11760.0
38	12821.5
39	13883.0
40	13883.0
41	14810.5
42	15738.0
43	15958.5
44	16179.0
45	17014.0
46	17849.0
47	17849.0
48	18833.0
49	19817.0
50	19576.5
51	19336.0
52	20582.0
53	21828.0
54	21828.0
55	21010.5
56	20193.0
57	19583.5
58	18974.0
59	17139.0
60	15304.0
61	15304.0
62	14165.5
63	13027.0
64	11644.0
65	10261.0
66	8579.5
67	6898.0
68	6898.0
69	5697.5
70	4497.0
71	3942.5
72	3388.0
73	2687.5
74	1987.0
75	1987.0
76	1499.5
77	1012.0
78	859.0
79	706.0
80	543.5
81	381.0
82	381.0
83	294.5
84	208.0
85	157.0
86	106.0
87	89.5
88	73.0
89	73.0
90	62.5
91	52.0
92	35.5
93	19.0
94	12.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	275347.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.17981346481066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.65248093388233	67.98763939262507
2	4.986611591176307	7.398118353098424
3	1.205585766476041	2.6829038181907054
4	0.5316812099240126	1.5776005189963238
5	0.3292556101378571	1.221205987113433
6	0.20799707259536634	0.9257510427810576
7	0.14292587939208526	0.7421550550819238
8	0.12672884007778462	0.7520577374065509
9	0.08684879440719963	0.5798184631892784
>10	0.6448288757599739	9.150838054837946
>50	0.061947882644849905	3.2436858317481123
>100	0.020649294214949952	2.2684647935094726
>500	0.0014749495867821393	0.6714218142847168
>1k	9.832997245214261E-4	0.7983391371370153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1155	0.41947070423865157	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.3755261542707929	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	728	0.2643936560049683	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	572	0.2077378725753322	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG	541	0.19647935150918658	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	305	0.11076932016691665	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	288	0.10459529248548197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.6317809890792343E-4	0.0
17	0.0	0.0	0.0	7.263561978158469E-4	0.0
18	0.0	0.0	0.0	7.263561978158469E-4	0.0
19	0.0	0.0	0.0	7.263561978158469E-4	0.0
20	0.0	0.0	0.0	0.0010895342967237704	0.0
21	0.0	0.0	0.0	0.0014527123956316937	0.0
22	0.0	0.0	0.0	0.002179068593447541	0.0
23	0.0	0.0	0.0	0.0029054247912633875	0.0
24	0.0	0.0	0.0	0.004358137186895082	0.0
25	0.0	0.0	0.0	0.005084493384710929	0.0
26	0.0	0.0	0.0	0.005084493384710929	0.0
27	0.0	0.0	0.0	0.006900383879250546	0.0
28	0.0	0.0	0.0	0.01597983635194863	0.0
29	0.0	0.0	0.0	0.03159649460498934	0.0
30	0.0	0.0	0.0	0.04757633095693797	0.0
31	0.0	0.0	0.0	0.1361917870904713	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTCG	25	0.0054891743	29.6	37
AACGCCG	40	5.9239843E-5	27.75	5
GGTATCA	380	0.0	27.263159	1
TAACGCC	35	8.8522E-4	26.428572	4
CGCCGCA	50	9.058336E-6	25.900002	10
TATACTG	45	1.3196444E-4	24.666666	5
CGAACGA	55	1.8958533E-5	23.545454	16
ATAACGC	40	0.0019274794	23.125	3
AATAACG	40	0.0019274794	23.125	2
CGCATCG	50	2.694875E-4	22.2	13
CCGGTCG	50	2.694875E-4	22.2	20
ATCGCCG	50	2.694875E-4	22.2	16
TAACGAA	60	3.7130518E-5	21.583334	13
GCGCAAG	155	0.0	21.48387	1
CGCAAGA	155	0.0	21.48387	2
ACACAGT	45	0.003818532	20.555555	6
TATTCCC	90	9.4127245E-8	20.555555	5
TCGCCGG	55	5.129452E-4	20.181818	17
CTGAGTA	55	5.129452E-4	20.181818	9
CGGCATC	55	5.129452E-4	20.181818	25
>>END_MODULE