FastQCFastQC Report
Fri 10 Feb 2017
ERR1630345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490487
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43700.17546768965266632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40140.16117329662833013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31480.1264009810129505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.021.4708041
TCTAGAC5650.018.663723
CTAGACA7500.016.5266694
AACACGC2400.016.187536
ACGGAGT3950.015.9240526
GTTCTAG6300.015.8571431
CTAGAAC6550.015.8167943
CTCTAGT5850.015.81196627
CGAACGA1659.749783E-1015.69697116
GACGGAC4050.015.5308647
GACGTAT1802.0190782E-1015.41666626
TTTCCGA3250.015.3692317
GTCTAGA6050.015.2892561
CCGATTT3050.015.16393520
CGATTTT3050.015.16393521
CGGAGTC4350.014.88505827
TAGACAT8100.014.8456795
ACGTATG2006.184564E-1114.827
GTTTCCG3550.014.5915516
TCGAAAT1651.6476406E-814.57575923
Produced by FastQC (version 0.11.5)