##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2490487
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258712051096833	31.0	31.0	34.0	28.0	34.0
2	31.473742685667503	31.0	31.0	34.0	30.0	34.0
3	31.622933988412708	31.0	31.0	34.0	30.0	34.0
4	35.37869942705985	37.0	35.0	37.0	33.0	37.0
5	35.168294393827395	37.0	35.0	37.0	32.0	37.0
6	35.22940774234116	37.0	35.0	37.0	32.0	37.0
7	35.22173012748109	37.0	35.0	37.0	32.0	37.0
8	35.19024190851027	37.0	35.0	37.0	32.0	37.0
9	36.82379430207827	39.0	37.0	39.0	33.0	39.0
10	36.63931110662292	38.0	35.0	39.0	32.0	39.0
11	36.766884549086186	38.0	37.0	39.0	33.0	39.0
12	36.60909452649221	38.0	35.0	39.0	32.0	39.0
13	36.675376743584685	38.0	35.0	39.0	32.0	39.0
14	37.745137798350285	39.0	37.0	40.0	33.0	41.0
15	37.71181861218308	39.0	37.0	40.0	33.0	41.0
16	37.68889177096688	39.0	37.0	40.0	33.0	41.0
17	37.62554954111385	39.0	37.0	40.0	32.0	41.0
18	37.59760440427916	39.0	37.0	40.0	32.0	41.0
19	37.59921573571755	39.0	37.0	40.0	32.0	41.0
20	37.53453521339401	39.0	37.0	40.0	32.0	41.0
21	37.484997111006805	39.0	37.0	40.0	32.0	41.0
22	37.45445408869831	39.0	36.0	40.0	32.0	41.0
23	37.38946479142433	39.0	36.0	40.0	32.0	41.0
24	37.37123020517674	39.0	36.0	40.0	32.0	41.0
25	37.34144085072518	39.0	36.0	40.0	32.0	41.0
26	37.14132818199814	39.0	36.0	40.0	31.0	41.0
27	37.027847565556456	39.0	36.0	40.0	31.0	41.0
28	36.93514601762627	39.0	36.0	40.0	31.0	41.0
29	36.86629723423572	39.0	36.0	40.0	31.0	41.0
30	36.802408123391125	39.0	36.0	40.0	30.0	41.0
31	36.682974855921756	39.0	36.0	40.0	30.0	41.0
32	36.568284837463516	38.0	35.0	40.0	30.0	41.0
33	36.499485442003916	38.0	35.0	40.0	30.0	41.0
34	36.39435018130992	38.0	35.0	40.0	30.0	41.0
35	36.30865971193586	38.0	35.0	40.0	30.0	41.0
36	36.20982201473045	38.0	35.0	40.0	30.0	41.0
37	36.14684798595616	38.0	35.0	40.0	29.0	41.0
38	36.03631337967233	38.0	35.0	40.0	29.0	41.0
39	35.93993102553838	38.0	35.0	40.0	29.0	41.0
40	35.83115832365316	38.0	35.0	40.0	28.0	41.0
41	35.704586693285286	38.0	34.0	40.0	28.0	41.0
42	35.64195677391611	38.0	34.0	40.0	27.0	41.0
43	34.448988089478085	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	4.0
13	5.0
14	6.0
15	22.0
16	40.0
17	107.0
18	169.0
19	366.0
20	775.0
21	1416.0
22	2515.0
23	4354.0
24	7097.0
25	10933.0
26	16340.0
27	23298.0
28	32280.0
29	43303.0
30	56475.0
31	73028.0
32	93217.0
33	118514.0
34	154849.0
35	206157.0
36	289149.0
37	428058.0
38	623134.0
39	304864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99616380250128	19.746981212911372	12.23953387429848	26.017321110288872
2	18.85663326088432	21.227535016243813	34.02587526054141	25.88995646233046
3	19.93622130932625	23.016903922807067	29.606498648657876	27.440376119208814
4	14.850589463024702	15.730136314704716	34.77649150547664	34.642782716793945
5	14.863157286105086	36.422394495534405	34.03992873682938	14.674519481531123
6	35.08257621902865	35.62146680548825	15.298694592664006	13.997262382819104
7	29.910856792265932	30.42123889825564	21.023880068436416	18.64402424104201
8	27.493337648419768	33.98086398363051	19.557178977444973	18.96861939050475
9	26.858923575991362	13.940084810721759	19.283497564934088	39.91749404835279
10	16.557203470646503	26.795321557590945	33.42711686509506	23.22035810666749
11	36.612959633999296	21.484553021156103	21.39489184243885	20.507595502405753
12	21.66202835027848	25.254538570167202	29.61019270528214	23.47324037427218
13	30.350931364026394	19.742042419815885	24.83658818536294	25.07043803079478
14	22.845893192777154	20.099884078897016	25.08256417319183	31.971658555134
15	25.19523290023196	27.83708567842354	21.94699269660914	25.02068872473536
16	25.064696181911412	26.38817227313373	23.722990724304122	24.82414082065074
17	23.78077058824238	26.38158721567308	25.36993768688614	24.467704509198402
18	24.214099491384616	25.30219993117812	26.11244306836374	24.371257509073526
19	24.57479199851274	25.257630334950555	26.039083922140527	24.128493744396177
20	24.577602693770334	25.395595319309034	25.93247826629892	24.09432372062171
21	24.982744338757843	25.088346174864597	25.775761929293346	24.153147557084218
22	24.767645845973096	25.243576858662582	25.77616345718729	24.21261383817703
23	24.409201895051048	25.21065157135934	25.937979198445927	24.44216733514369
24	24.40426310195556	25.564598409869234	25.591460625973955	24.439677862201247
25	25.03622785423092	25.094850926746453	25.764318384316	24.104602834706625
26	24.890633839887542	25.309668350005442	25.581261817467826	24.21843599263919
27	24.549495741194395	25.234662939417067	25.577326844107194	24.63851447528134
28	24.316850479444383	25.04301367563854	26.218165362838675	24.421970482078404
29	24.155034738185744	25.26036072462936	26.032619323048063	24.551985214136835
30	23.98045040989975	25.954401689308153	25.81768144142089	24.2474664593712
31	24.745883034121437	25.451166779830615	25.633701360416655	24.169248825631293
32	24.289907957760875	25.52002881364167	25.626433705536307	24.563629523061152
33	24.035419578580413	25.240204024353467	26.32898706156667	24.39538933549944
34	24.84783096639332	24.890392923151175	25.810614550487514	24.45116155996799
35	24.332831289623275	25.281521244640103	26.36335784928811	24.02228961644851
36	24.569973663785436	25.163231127084785	25.968816540700672	24.297978668429106
37	24.586275696279483	24.868228583405575	26.222983697565976	24.322512022748967
38	24.167642714055525	25.005430664765566	26.49566129034201	24.331265330836903
39	24.1650327827449	24.95636395612585	26.50236680617084	24.37623645495841
40	23.86970901675054	24.98073669928813	26.819533689595648	24.33002059436568
41	23.855013095832263	24.658992397872385	26.964164036993566	24.521830469301786
42	23.937205855722194	24.887461769525398	26.861975187985323	24.31335718676709
43	23.7764742397772	24.36804528592199	27.083939807756472	24.771540666544333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	356.0
1	348.0
2	340.0
3	606.0
4	872.0
5	872.0
6	1172.0
7	1472.0
8	1371.5
9	1271.0
10	1791.0
11	2311.0
12	2311.0
13	4029.0
14	5747.0
15	8166.0
16	10585.0
17	11093.5
18	11602.0
19	11602.0
20	15045.0
21	18488.0
22	19568.0
23	20648.0
24	24754.0
25	28860.0
26	28860.0
27	34207.0
28	39554.0
29	46364.5
30	53175.0
31	60508.5
32	67842.0
33	67842.0
34	78563.0
35	89284.0
36	100010.0
37	110736.0
38	119061.0
39	127386.0
40	127386.0
41	137533.0
42	147680.0
43	155047.5
44	162415.0
45	167556.0
46	172697.0
47	172697.0
48	177355.5
49	182014.0
50	186071.5
51	190129.0
52	189697.5
53	189266.0
54	189266.0
55	180281.5
56	171297.0
57	161969.0
58	152641.0
59	146002.5
60	139364.0
61	139364.0
62	129455.5
63	119547.0
64	105446.5
65	91346.0
66	78882.5
67	66419.0
68	66419.0
69	55795.5
70	45172.0
71	37884.0
72	30596.0
73	24148.5
74	17701.0
75	17701.0
76	13560.5
77	9420.0
78	7338.0
79	5256.0
80	4221.5
81	3187.0
82	3187.0
83	2349.0
84	1511.0
85	1250.5
86	990.0
87	861.5
88	733.0
89	733.0
90	591.5
91	450.0
92	269.0
93	88.0
94	60.5
95	33.0
96	33.0
97	19.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2490487.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.61341516855836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3507875036421	43.61493544721933
2	10.166161871663396	10.900853141925161
3	3.1276072488239297	5.0304511774617
4	1.4446113876567452	3.098022003346732
5	0.8600294869512625	2.305455897056014
6	0.5602214014766499	1.802122955020755
7	0.3902133682210105	1.4644469920328205
8	0.28215219787782275	1.2101714340435954
9	0.2118012929748206	1.0219851588146869
>10	1.377214131824682	14.114819013156488
>50	0.13902362475956087	5.149059743889754
>100	0.08664845980147769	8.421898980466652
>500	0.002702316505578541	0.949288686065908
>1k	8.257078211488442E-4	0.9164893695004083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4370	0.17546768965266632	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4014	0.16117329662833013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3148	0.1264009810129505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.015278939420282E-5
9	0.0	0.0	0.0	0.0	4.015278939420282E-5
10	0.0	0.0	0.0	0.0	8.030557878840564E-5
11	0.0	0.0	0.0	4.015278939420282E-5	8.030557878840564E-5
12	0.0	0.0	0.0	4.015278939420282E-5	8.030557878840564E-5
13	0.0	0.0	0.0	8.030557878840564E-5	8.030557878840564E-5
14	0.0	0.0	0.0	8.030557878840564E-5	8.030557878840564E-5
15	0.0	0.0	0.0	1.2045836818260846E-4	8.030557878840564E-5
16	0.0	0.0	0.0	3.2122231515362257E-4	8.030557878840564E-5
17	0.0	0.0	0.0	5.621390515188395E-4	8.030557878840564E-5
18	0.0	0.0	0.0	5.621390515188395E-4	8.030557878840564E-5
19	0.0	0.0	0.0	6.022918409130423E-4	8.030557878840564E-5
20	0.0	0.0	0.0	6.424446303072451E-4	8.030557878840564E-5
21	0.0	0.0	0.0	7.629029984898536E-4	8.030557878840564E-5
22	0.0	0.0	0.0	0.0012045836818260847	8.030557878840564E-5
23	0.0	0.0	0.0	0.0019273338909217353	8.030557878840564E-5
24	0.0	0.0	0.0	0.0030114592045652117	8.030557878840564E-5
25	0.0	0.0	0.0	0.0036539038348724568	8.030557878840564E-5
26	0.0	0.0	0.0	0.005059251463669556	8.030557878840564E-5
27	0.0	0.0	0.0	0.010479878031886936	8.030557878840564E-5
28	0.0	0.0	0.0	0.026099313106231835	8.030557878840564E-5
29	0.0	0.0	0.0	0.04770151380031295	8.030557878840564E-5
30	0.0	0.0	0.0	0.08223291267932738	8.030557878840564E-5
31	0.0	0.0	0.0	0.18442176168757354	8.030557878840564E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1370	0.0	21.470804	1
TCTAGAC	565	0.0	18.66372	3
CTAGACA	750	0.0	16.526669	4
AACACGC	240	0.0	16.1875	36
ACGGAGT	395	0.0	15.92405	26
GTTCTAG	630	0.0	15.857143	1
CTAGAAC	655	0.0	15.816794	3
CTCTAGT	585	0.0	15.811966	27
CGAACGA	165	9.749783E-10	15.696971	16
GACGGAC	405	0.0	15.530864	7
GACGTAT	180	2.0190782E-10	15.416666	26
TTTCCGA	325	0.0	15.36923	17
GTCTAGA	605	0.0	15.289256	1
CCGATTT	305	0.0	15.163935	20
CGATTTT	305	0.0	15.163935	21
CGGAGTC	435	0.0	14.885058	27
TAGACAT	810	0.0	14.845679	5
ACGTATG	200	6.184564E-11	14.8	27
GTTTCCG	355	0.0	14.59155	16
TCGAAAT	165	1.6476406E-8	14.575759	23
>>END_MODULE