FastQCFastQC Report
Fri 10 Feb 2017
ERR1630344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379428
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8310.21901388405705433No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG6570.1731553812581043No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT5520.14548214681046207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.1436372645139526No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5140.13546707148655346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4690.12360711386613535No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4300.11332848392843965No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3800.1001507532390862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCA250.0054917629.67
TCCGTCC451.3209308E-424.6666668
ACTACAT400.00192881623.1250023
AATTGAC450.00382115620.55555531
CTACATC450.00382115620.5555554
ATACCGC450.00382115620.5555556
TCAGTAC450.00382115620.5555553
GGTATCA3350.020.4328371
ACGCAAT609.221276E-418.511
ATAGAAC500.00702640918.53
GGATAAT500.00702640918.51
CAAGACG902.144494E-618.54
TAGAACA2150.018.0697674
GTAGAAC1850.018.03
ACTATTC1054.776357E-717.6190478
GTATCAA3850.017.29872
GATACCG650.001577297617.0769235
TTATGAT650.001577297617.0769234
CTGCGTC650.001577297617.07692323
GACGCAA650.001577297617.07692310
Produced by FastQC (version 0.11.5)