FastQCFastQC Report
Fri 10 Feb 2017
ERR1630342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682974
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70210.2616872172447441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66230.24685293260389404No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG53860.20074737958698072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52040.19396386248990857No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT38510.14353474912541084No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC36220.13499944464612776No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA36160.13477581221435617No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG35320.13164495816955363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.10603904473170446No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC27700.10324363933455934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG609.2411967E-418.55
TCTAGCG4350.018.28735728
CTAGCGG4450.017.87640629
ACGGACC6300.017.6190478
AAGACGG6550.016.9465645
GACGGAC6450.016.9224827
ATCTCGC8500.016.75882311
GGTATCA29950.016.430721
GCGCAAG6900.016.3550721
ATCGTAC803.3846716E-416.187525
CAAGACG6900.015.818844
CGGACCA6850.015.6642349
GTATTAG6650.015.5789471
TCGCCAT9550.015.30366613
GTAGAAC11450.015.1877743
CGAGCCG6000.015.10833415
TCTCGCC9850.015.02538112
TAGAACA12100.014.9834724
AGACGGA7450.014.8993286
CGCAATA5850.014.86324836
Produced by FastQC (version 0.11.5)