##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630342.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2682974
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89042159931479	33.0	31.0	34.0	30.0	34.0
2	32.084779800326054	33.0	31.0	34.0	30.0	34.0
3	32.23096943913732	34.0	31.0	34.0	30.0	34.0
4	35.87067783735512	37.0	35.0	37.0	35.0	37.0
5	35.78833563053537	37.0	35.0	37.0	35.0	37.0
6	35.849907229812885	37.0	35.0	37.0	35.0	37.0
7	35.825646092731425	37.0	35.0	37.0	35.0	37.0
8	35.80840328680039	37.0	35.0	37.0	35.0	37.0
9	37.51914852697045	39.0	37.0	39.0	35.0	39.0
10	37.44504046628853	39.0	37.0	39.0	34.0	39.0
11	37.516642725572446	39.0	37.0	39.0	35.0	39.0
12	37.40565767689139	39.0	37.0	39.0	34.0	39.0
13	37.43645782627785	39.0	37.0	39.0	34.0	39.0
14	38.673178718839615	40.0	38.0	41.0	35.0	41.0
15	38.678795992805	40.0	38.0	41.0	35.0	41.0
16	38.659136093007234	40.0	38.0	41.0	34.0	41.0
17	38.634906637186944	40.0	38.0	41.0	34.0	41.0
18	38.6048668380685	40.0	38.0	41.0	34.0	41.0
19	38.63121744750415	40.0	38.0	41.0	34.0	41.0
20	38.58763707736266	40.0	38.0	41.0	34.0	41.0
21	38.54121433901335	40.0	38.0	41.0	34.0	41.0
22	38.52081123410066	40.0	38.0	41.0	34.0	41.0
23	38.470135379619784	40.0	38.0	41.0	34.0	41.0
24	38.46248789589463	40.0	38.0	41.0	34.0	41.0
25	38.43069742755614	40.0	38.0	41.0	34.0	41.0
26	38.285913691299285	40.0	38.0	41.0	34.0	41.0
27	38.18483816839075	40.0	38.0	41.0	34.0	41.0
28	38.109935839855325	40.0	38.0	41.0	33.0	41.0
29	38.05245149598915	40.0	38.0	41.0	33.0	41.0
30	38.0001520700536	40.0	37.0	41.0	33.0	41.0
31	37.896380285459344	40.0	37.0	41.0	33.0	41.0
32	37.79778223717412	40.0	37.0	41.0	33.0	41.0
33	37.74039703701937	40.0	37.0	41.0	33.0	41.0
34	37.65954049498802	40.0	37.0	41.0	33.0	41.0
35	37.57455197105898	40.0	37.0	41.0	32.0	41.0
36	37.47197885629902	40.0	37.0	41.0	32.0	41.0
37	37.425137179860855	40.0	37.0	41.0	32.0	41.0
38	37.31402950606305	40.0	36.0	41.0	31.0	41.0
39	37.22778491330889	39.0	36.0	41.0	31.0	41.0
40	37.13002735024641	39.0	36.0	41.0	31.0	41.0
41	37.006111874360315	39.0	36.0	41.0	31.0	41.0
42	36.9492156837897	39.0	36.0	41.0	31.0	41.0
43	35.803418147175485	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	4.0
12	3.0
13	2.0
14	1.0
15	11.0
16	24.0
17	53.0
18	118.0
19	219.0
20	429.0
21	808.0
22	1395.0
23	2443.0
24	3923.0
25	6162.0
26	9375.0
27	13880.0
28	20131.0
29	28310.0
30	38712.0
31	51641.0
32	67683.0
33	88677.0
34	118050.0
35	159215.0
36	224807.0
37	340956.0
38	620424.0
39	885510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03145464697012	19.76381433439161	12.71980272637752	25.48492829226075
2	17.691375317092152	21.6422522171292	35.61271931818944	25.053653147589205
3	19.21185222070732	23.48185260088245	31.172273752932377	26.134021425477847
4	14.24743586780938	15.809061138870522	35.5109292896614	34.432573703658704
5	13.828236874453498	37.43454092361686	34.74849178560806	13.98873041632159
6	33.72302526971935	35.998969799930975	16.061989419204213	14.21601551114547
7	28.171648327564856	31.69352367932004	21.606098307326125	18.528729685788978
8	26.328059832111677	34.76336334232087	20.42069733064875	18.487879494918698
9	26.001183761005507	14.840881797587304	20.13176422879983	39.026170212607354
10	16.46281328108286	27.53224593305787	33.33509754473953	22.669843241119743
11	35.19042674286072	22.289444474676237	21.968830111659674	20.55129867080337
12	20.79177807910177	26.41531375257457	29.43543247157818	23.357475696745478
13	29.05380372675993	19.869443386331735	26.080237825636775	24.99651506127156
14	22.171142918269055	21.611502757760604	25.24702065692772	30.970333667042617
15	24.75126482776203	28.62439218568648	22.78691481915218	23.83742816739931
16	24.640790406466852	27.352818178633115	24.0383619073461	23.968029507553933
17	23.28568968614679	27.361018034464742	25.577847567661856	23.775444711726614
18	22.68557205548768	25.85481633441099	27.366161580395488	24.09345002970584
19	23.8036596702018	26.357094776170026	27.09306910913039	22.746176444497785
20	24.10675615939625	25.36293680072934	27.233137555563342	23.297169484311066
21	24.122224069260454	26.518408303621282	25.923881483756457	23.435486143361807
22	23.94946801571689	25.84359744075045	26.31784728439411	23.889087259138552
23	22.946662919581033	26.304652970919584	26.626050047447347	24.122634062052036
24	24.21514334466156	26.408194786829835	26.389074213913368	22.987587654595238
25	24.102208966616896	25.67810944123946	26.551021366587975	23.66866022555567
26	23.920507615802464	26.355119356356045	26.321835396094034	23.402537631747457
27	24.11305513955782	25.609267924325767	26.210988254079243	24.06668868203717
28	23.033618663468225	26.124181598479897	27.139472838723	23.70272689932888
29	23.10607557136223	26.667571135612945	26.716919358890546	23.509433934134286
30	22.753444498530364	27.452595515275213	26.664179377064407	23.12978060913002
31	23.87306026819492	26.404020314770104	26.385756999508754	23.337162417526223
32	22.791275651571727	26.41348742104843	26.442298732675006	24.352938194704834
33	22.904545478264048	25.983740431327323	26.96843130048968	24.14328278991895
34	23.532207170103028	25.995481133995334	26.768168457838204	23.704143238063434
35	24.06206694511389	25.971142471004192	26.74558158222927	23.221209001652642
36	22.98460588883828	26.838128136910754	26.645207892435778	23.53205808181518
37	24.249060930146918	25.452464317581907	26.51591107479983	23.782563677471344
38	23.31744549145836	26.001631025869052	27.376933209192483	23.303990273480103
39	23.582263562747908	25.622834958519913	27.160345199021684	23.6345562797105
40	23.22016538363771	25.314818555826484	27.999563171316606	23.4654528892192
41	22.396340777063063	26.099134766121473	28.173847379810613	23.330677077004847
42	23.641749789599153	25.27542197576272	27.737018696416737	23.345809538221392
43	22.5572815837947	25.228794613738337	27.883199762651444	24.330724039815518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	218.0
2	265.0
3	693.5
4	1122.0
5	1122.0
6	1541.5
7	1961.0
8	1859.0
9	1757.0
10	2517.0
11	3277.0
12	3277.0
13	5776.5
14	8276.0
15	13410.0
16	18544.0
17	18442.5
18	18341.0
19	18341.0
20	21504.0
21	24667.0
22	27010.5
23	29354.0
24	33533.0
25	37712.0
26	37712.0
27	43388.5
28	49065.0
29	63180.5
30	77296.0
31	81984.0
32	86672.0
33	86672.0
34	96903.5
35	107135.0
36	115941.5
37	124748.0
38	142550.5
39	160353.0
40	160353.0
41	171096.5
42	181840.0
43	171881.0
44	161922.0
45	172586.0
46	183250.0
47	183250.0
48	191328.0
49	199406.0
50	201606.5
51	203807.0
52	212674.5
53	221542.0
54	221542.0
55	196961.0
56	172380.0
57	161447.0
58	150514.0
59	136423.5
60	122333.0
61	122333.0
62	111312.0
63	100291.0
64	87422.5
65	74554.0
66	63802.0
67	53050.0
68	53050.0
69	45354.0
70	37658.0
71	33092.5
72	28527.0
73	22746.5
74	16966.0
75	16966.0
76	12866.0
77	8766.0
78	7303.5
79	5841.0
80	4663.0
81	3485.0
82	3485.0
83	2627.5
84	1770.0
85	1721.5
86	1673.0
87	1539.0
88	1405.0
89	1405.0
90	1241.0
91	1077.0
92	612.0
93	147.0
94	94.5
95	42.0
96	42.0
97	25.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2682974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.273409166323084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97587689483439	35.75507188928998
2	12.555042022639984	11.36819109182721
3	3.745665523830144	5.087371435816559
4	1.5317553672156732	2.7739114993066654
5	0.7730431037520078	1.7499148369684496
6	0.4705378090597823	1.2781710454673239
7	0.3047070397406286	0.9656588540225582
8	0.22679219129274972	0.8214124537698937
9	0.16281692045331286	0.6634149352995159
>10	0.9710001029274683	8.312906073379896
>50	0.11560387143998244	3.7067222110165776
>100	0.1349252083046261	13.326267776314833
>500	0.023495330396884197	7.127217743517296
>1k	0.008408855089411044	6.159275593839129
>5k	3.2975902311415864E-4	0.9044925601642021
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7021	0.2616872172447441	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6623	0.24685293260389404	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5386	0.20074737958698072	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5204	0.19396386248990857	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3851	0.14353474912541084	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3622	0.13499944464612776	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3616	0.13477581221435617	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3532	0.13164495816955363	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2845	0.10603904473170446	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2770	0.10324363933455934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	3.727207196193478E-5	0.0
12	0.0	0.0	0.0	3.727207196193478E-5	0.0
13	0.0	0.0	0.0	3.727207196193478E-5	0.0
14	0.0	0.0	0.0	3.727207196193478E-5	0.0
15	0.0	0.0	0.0	2.6090450373354344E-4	0.0
16	0.0	0.0	0.0	7.081693672767608E-4	0.0
17	0.0	0.0	0.0	0.001155434230819978	0.0
18	0.0	0.0	0.0	0.0012672504467057825	0.0
19	0.0	0.0	0.0	0.0015281549504393259	0.0
20	0.0	0.0	0.0	0.0018263315261348042	0.0
21	0.0	0.0	0.0	0.002310868461639956	0.0
22	0.0	0.0	0.0	0.0032426702606883255	0.0
23	0.0	0.0	0.0	0.004770825211127652	0.0
24	0.0	0.0	0.0	0.006671700881186325	0.0
25	0.0	0.0	0.0	0.00756623060827276	0.0
26	0.0	0.0	0.0	0.009243473846559824	0.0
27	0.0	0.0	0.0	0.015467909864202933	0.0
28	0.0	0.0	0.0	0.0301158341452433	0.0
29	0.0	0.0	0.0	0.05232998903455643	0.0
30	0.0	0.0	0.0	0.08602394208814547	0.0
31	0.0	0.0	0.0	0.21211536153537083	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	60	9.2411967E-4	18.5	5
TCTAGCG	435	0.0	18.287357	28
CTAGCGG	445	0.0	17.876406	29
ACGGACC	630	0.0	17.619047	8
AAGACGG	655	0.0	16.946564	5
GACGGAC	645	0.0	16.922482	7
ATCTCGC	850	0.0	16.758823	11
GGTATCA	2995	0.0	16.43072	1
GCGCAAG	690	0.0	16.355072	1
ATCGTAC	80	3.3846716E-4	16.1875	25
CAAGACG	690	0.0	15.81884	4
CGGACCA	685	0.0	15.664234	9
GTATTAG	665	0.0	15.578947	1
TCGCCAT	955	0.0	15.303666	13
GTAGAAC	1145	0.0	15.187774	3
CGAGCCG	600	0.0	15.108334	15
TCTCGCC	985	0.0	15.025381	12
TAGAACA	1210	0.0	14.983472	4
AGACGGA	745	0.0	14.899328	6
CGCAATA	585	0.0	14.863248	36
>>END_MODULE