FastQCFastQC Report
Fri 10 Feb 2017
ERR1630341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736751
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.2785201513129945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17270.23440755424831458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13730.18635875621478626No Hit
ATGTAGAACTGTGGGTGCACGATGATCCTGCTGACTGGCAGCA8790.11930760867647279No Hit
GTTCTACATCATCCAGACTGGAGCGGATATCGCCCTGCTGGAG7560.10261268732584007No Hit
CCCCAGGCCTGACTCACGGCTTTTTGGGGACATAGTGGTGGAT7530.1022054941221661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG250.005495079329.59999835
CGATAGA358.866362E-426.4285737
GACGGAC1301.8189894E-1221.3461537
GTCCTAT806.9531416E-720.81251
TAAACGC806.9531416E-720.812528
ATAGGCG450.003824523220.55555732
ACGTGAC450.003824523220.55555730
AAGACGG1351.8189894E-1220.5555555
ACGGACC1351.8189894E-1220.5555558
CGCAAGA1450.020.4137942
TAATACT555.1406847E-420.1818184
GTACTAG555.1406847E-420.1818181
TAGGCGA555.1406847E-420.18181833
CCATTAA555.1406847E-420.1818181
TAGAACT3150.019.9682544
CGAAAGC1403.6379788E-1219.82142819
TTAGACA759.258369E-619.7333344
ATTAGAG1103.8460712E-818.53
GCGAAAG1501.2732926E-1118.518
AGACGGA1501.2732926E-1118.56
Produced by FastQC (version 0.11.5)