##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630341.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736751
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841039917149757	31.0	31.0	34.0	30.0	34.0
2	32.02949843298482	33.0	31.0	34.0	30.0	34.0
3	32.17432619704622	34.0	31.0	34.0	30.0	34.0
4	35.826976821205534	37.0	35.0	37.0	35.0	37.0
5	35.7351004613499	37.0	35.0	37.0	35.0	37.0
6	35.79332637485392	37.0	35.0	37.0	35.0	37.0
7	35.76463146979101	37.0	35.0	37.0	35.0	37.0
8	35.7471438790039	37.0	35.0	37.0	33.0	37.0
9	37.46309268667433	39.0	37.0	39.0	35.0	39.0
10	37.35654108375829	39.0	37.0	39.0	34.0	39.0
11	37.44501330843121	39.0	37.0	39.0	35.0	39.0
12	37.30576816319218	39.0	37.0	39.0	34.0	39.0
13	37.354182077798335	39.0	37.0	39.0	34.0	39.0
14	38.55579157680139	40.0	38.0	41.0	34.0	41.0
15	38.55413972970515	40.0	38.0	41.0	34.0	41.0
16	38.525167933263745	40.0	38.0	41.0	34.0	41.0
17	38.5034468904691	40.0	38.0	41.0	34.0	41.0
18	38.47164645857284	40.0	38.0	41.0	34.0	41.0
19	38.456106608609964	40.0	38.0	41.0	34.0	41.0
20	38.40671000107228	40.0	38.0	41.0	34.0	41.0
21	38.368036147898	40.0	38.0	41.0	34.0	41.0
22	38.32375660161981	40.0	38.0	41.0	34.0	41.0
23	38.27274886630626	40.0	38.0	41.0	34.0	41.0
24	38.25181642101606	40.0	38.0	41.0	34.0	41.0
25	38.19933057437316	40.0	38.0	41.0	33.0	41.0
26	38.02825242178158	40.0	37.0	41.0	33.0	41.0
27	37.898393079887235	40.0	37.0	41.0	33.0	41.0
28	37.83222554160089	40.0	37.0	41.0	33.0	41.0
29	37.755290457698734	40.0	37.0	41.0	33.0	41.0
30	37.662314676193176	40.0	37.0	41.0	32.0	41.0
31	37.56321335159369	40.0	37.0	41.0	32.0	41.0
32	37.44507574472244	40.0	37.0	41.0	32.0	41.0
33	37.37486477792361	40.0	36.0	41.0	31.0	41.0
34	37.2660261065136	39.0	36.0	41.0	31.0	41.0
35	37.17193461562997	39.0	36.0	41.0	31.0	41.0
36	37.03910819259153	39.0	36.0	41.0	31.0	41.0
37	36.96304450214523	39.0	36.0	41.0	31.0	41.0
38	36.8237993569062	39.0	35.0	41.0	30.0	41.0
39	36.68836825467492	39.0	35.0	41.0	30.0	41.0
40	36.5833531274474	39.0	35.0	40.0	30.0	41.0
41	36.454414042193356	39.0	35.0	40.0	30.0	41.0
42	36.35782238503918	39.0	35.0	40.0	30.0	41.0
43	35.06609424350968	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	1.0
13	1.0
14	2.0
15	1.0
16	7.0
17	9.0
18	31.0
19	72.0
20	135.0
21	282.0
22	464.0
23	816.0
24	1341.0
25	2206.0
26	3275.0
27	4710.0
28	6671.0
29	9199.0
30	12343.0
31	15986.0
32	20735.0
33	26830.0
34	35563.0
35	47817.0
36	65385.0
37	99571.0
38	173079.0
39	210215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.865837983253506	17.869130818960542	11.401138240735337	26.863892957050616
2	20.094170214903002	19.440353660870496	32.529850655106	27.935625469120502
3	20.67401333693473	20.96135600766066	28.31879427377771	30.0458363816269
4	15.196314630044617	14.48467664109041	32.63952135796219	37.679487370902784
5	16.07897376454189	35.30840134590927	32.72964678704203	15.882978102506817
6	37.49489311857059	33.07087469172082	14.455358730425882	14.978873459282715
7	32.18346496984734	28.659479254184927	19.24666542698958	19.91039034897815
8	29.717095735194114	31.874676790394584	18.09335854311701	20.314868931294292
9	27.590325632404976	12.966117453522289	18.087793569333467	41.355763344739266
10	18.661257331174305	25.28167589864147	30.814888612299136	25.242178157885093
11	39.229400435153806	19.801941225732982	18.868925865048027	22.09973247406519
12	23.35904532196088	24.132169484669856	27.452694329563176	25.05609086380609
13	32.71240894141983	17.72932781903248	22.531357269959592	27.026905969588093
14	23.700951881979123	18.855081296123114	23.3602669015719	34.08369992032586
15	27.325107125745333	25.692533841148503	20.247410590552303	26.734948442553865
16	26.86796488908736	24.04883060898458	21.963458481902297	27.119746020025765
17	25.76786458382819	24.43403537966016	23.231458118143035	26.56664191836862
18	25.79826834303584	22.992299976518527	24.275772954498876	26.933658725946756
19	26.0163881691372	23.882560050817712	24.22894573607637	25.872106043968724
20	27.207292558815666	23.032340641546465	24.04842341578091	25.711943383856962
21	27.796908317735568	22.723416731025814	23.488939953932874	25.990734997305736
22	26.785168937673653	23.72348323924908	23.756873081950346	25.734474741126924
23	25.782387808092555	23.21815647348969	23.729455406236298	27.27000031218146
24	26.295858437925435	23.535088517015925	23.8670867090781	26.30196633598054
25	26.93596615410091	23.403700843297127	23.86993706150382	25.790395941098144
26	27.216658002500164	23.245574149203733	23.694572521788228	25.843195326507875
27	26.654459919294304	23.828539085796965	23.621006282991132	25.8959947119176
28	25.72130882754146	23.372889890885794	23.795013512027808	27.110787769544935
29	25.382116888881047	24.07421231868026	24.23844691082876	26.305223881609933
30	25.72945269161494	24.422498238889396	24.230574508891063	25.617474560604602
31	26.862535646371704	23.86681524694232	23.161828080314788	26.10882102637119
32	25.667287862520716	23.939295637196285	23.445506012207655	26.94791048807535
33	25.568611376163723	23.301088155971282	24.277537458381463	26.85276300948353
34	25.812927298368106	23.4244676966845	24.054531313836016	26.708073691111377
35	26.10461336326656	23.405872540383385	24.454395039843853	26.0351190565062
36	25.656565108157302	23.708145628577363	24.587682948513134	26.0476063147522
37	26.675498234817464	23.079575053172647	23.783069178053374	26.46185753395652
38	25.724702104238744	23.071974113370732	25.09015936184681	26.11316442054371
39	25.498981338335476	22.159454143937367	25.734203278991135	26.607361238736015
40	25.5439083218075	22.77947366206493	25.2702744889386	26.40634352718897
41	24.800916456170402	22.727217200926773	26.293143816567603	26.178722526335218
42	24.582796630069044	22.896609573655144	26.37851865827125	26.14207513800456
43	24.379742952503626	22.411778199147335	26.54791103099962	26.660567817349417
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	24.0
2	32.0
3	99.0
4	166.0
5	166.0
6	237.0
7	308.0
8	296.5
9	285.0
10	427.5
11	570.0
12	570.0
13	1109.5
14	1649.0
15	2841.0
16	4033.0
17	3683.0
18	3333.0
19	3333.0
20	3858.5
21	4384.0
22	3872.0
23	3360.0
24	4090.5
25	4821.0
26	4821.0
27	5805.5
28	6790.0
29	8080.0
30	9370.0
31	11096.5
32	12823.0
33	12823.0
34	15099.5
35	17376.0
36	20141.0
37	22906.0
38	25473.0
39	28040.0
40	28040.0
41	30957.5
42	33875.0
43	36199.0
44	38523.0
45	41040.0
46	43557.0
47	43557.0
48	46556.0
49	49555.0
50	50274.0
51	50993.0
52	51831.0
53	52669.0
54	52669.0
55	53423.0
56	54177.0
57	53886.5
58	53596.0
59	52456.5
60	51317.0
61	51317.0
62	49836.5
63	48356.0
64	45001.0
65	41646.0
66	36672.0
67	31698.0
68	31698.0
69	28951.5
70	26205.0
71	22625.5
72	19046.0
73	14253.5
74	9461.0
75	9461.0
76	7245.5
77	5030.0
78	3947.5
79	2865.0
80	2359.5
81	1854.0
82	1854.0
83	1409.5
84	965.0
85	744.0
86	523.0
87	417.0
88	311.0
89	311.0
90	262.5
91	214.0
92	124.5
93	35.0
94	25.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	736751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.53937554439872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0725204578331	49.215528480452356
2	9.462958994589554	11.079114206910475
3	2.5604222410258974	4.49656557358938
4	1.1034993348248476	2.583926478972238
5	0.600742747962258	1.7583552664268347
6	0.3565332901920782	1.2522741701180495
7	0.2602471193959339	1.0664292699666587
8	0.18337202996919372	0.8587587301364293
9	0.15406046103627052	0.8116742866630902
>10	1.029491830459924	11.759664752024886
>50	0.1364559694019109	5.62661805160783
>100	0.07761046601329609	8.234436306619848
>500	0.0013900381971708225	0.5579404608968741
>1k	6.950190985854113E-4	0.6987139656151424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2052	0.2785201513129945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1727	0.23440755424831458	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.18635875621478626	No Hit
ATGTAGAACTGTGGGTGCACGATGATCCTGCTGACTGGCAGCA	879	0.11930760867647279	No Hit
GTTCTACATCATCCAGACTGGAGCGGATATCGCCCTGCTGGAG	756	0.10261268732584007	No Hit
CCCCAGGCCTGACTCACGGCTTTTTGGGGACATAGTGGTGGAT	753	0.1022054941221661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.3573106789132285E-4	0.0
11	0.0	0.0	0.0	1.3573106789132285E-4	0.0
12	0.0	0.0	0.0	1.3573106789132285E-4	0.0
13	0.0	0.0	0.0	4.0719320367396856E-4	0.0
14	0.0	0.0	0.0	4.0719320367396856E-4	0.0
15	0.0	0.0	0.0	4.0719320367396856E-4	0.0
16	0.0	0.0	0.0	8.143864073479371E-4	0.0
17	0.0	0.0	0.0	0.0014930417468045514	0.0
18	0.0	0.0	0.0	0.0014930417468045514	0.0
19	0.0	0.0	0.0	0.001764503882587197	0.0
20	0.0	0.0	0.0	0.0020359660183698427	0.0
21	0.0	0.0	0.0	0.002986083493609103	0.0
22	0.0	0.0	0.0	0.004207663104631008	0.0
23	0.0	0.0	0.0	0.006515091258783497	0.0
24	0.0	0.0	0.0	0.008279595141370694	0.0
25	0.0	0.0	0.0	0.009229712616609953	0.0
26	0.0	0.0	0.0	0.01235152717811038	0.0
27	0.0	0.0	0.0	0.021581239794720333	0.0
28	0.0	0.0	0.0	0.04886318444087623	0.0
29	0.0	0.0	0.0	0.08998969801194705	0.0
30	0.0	0.0	0.0	0.14509651157582412	0.0
31	0.0	0.0	0.0	0.29236472023790944	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	25	0.0054950793	29.599998	35
CGATAGA	35	8.866362E-4	26.42857	37
GACGGAC	130	1.8189894E-12	21.346153	7
GTCCTAT	80	6.9531416E-7	20.8125	1
TAAACGC	80	6.9531416E-7	20.8125	28
ATAGGCG	45	0.0038245232	20.555557	32
ACGTGAC	45	0.0038245232	20.555557	30
AAGACGG	135	1.8189894E-12	20.555555	5
ACGGACC	135	1.8189894E-12	20.555555	8
CGCAAGA	145	0.0	20.413794	2
TAATACT	55	5.1406847E-4	20.181818	4
GTACTAG	55	5.1406847E-4	20.181818	1
TAGGCGA	55	5.1406847E-4	20.181818	33
CCATTAA	55	5.1406847E-4	20.181818	1
TAGAACT	315	0.0	19.968254	4
CGAAAGC	140	3.6379788E-12	19.821428	19
TTAGACA	75	9.258369E-6	19.733334	4
ATTAGAG	110	3.8460712E-8	18.5	3
GCGAAAG	150	1.2732926E-11	18.5	18
AGACGGA	150	1.2732926E-11	18.5	6
>>END_MODULE