FastQCFastQC Report
Fri 10 Feb 2017
ERR1630340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062469
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG63380.20695719695448347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56740.1852753448279803No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT50190.16388737322728816No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG44530.14540555349294965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.13136459503753345No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC39650.1294706983156401No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA37180.12140531055171498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35250.11510320594265606No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC33740.110172543787382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.024.5647371
GTATCAA28850.015.2616972
TCTAGCG2650.014.660377528
TAGTCGG908.279562E-414.38888830
CGGCCTT10050.014.1741324
CGCAATA2551.8189894E-1213.78431336
TACACCG1652.599154E-713.4545455
GACGGTA3350.013.2537328
CTAGCGG2800.013.21428629
ATCTCGC11100.013.16666711
TCGCCAT9850.013.14720813
CGGACCA4950.013.0808099
AACGGTA850.00940940413.05882418
AATACCG850.00940940413.0588245
TTCCTCG10850.012.95852619
GCTTAGG19600.012.9311231
TAGGACA20800.012.8966354
ACGGTAT3450.012.8695659
GCGCAAG5050.012.8217821
ACGGACC4500.012.7444458
Produced by FastQC (version 0.11.5)