FastQCFastQC Report
Fri 10 Feb 2017
ERR1630339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339593
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT151534.462106109372101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119073.5062560182335916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93052.740044700568033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48931.4408424201912289No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA25280.7444205269248777No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.4508337922159762No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15110.4449443893130895No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12910.3801609573813359No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12600.3710323828818615No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.2980037868860666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.2909365034026025No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA9240.27209041411336515No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8170.24058210858292134No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7990.2352816459703233No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.20465675087531252No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6820.20082863898843614No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA6000.17668208708660071No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.17255950505458004No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5850.1722650349094357No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.1696148036031367No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA5700.1678479827322707No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5290.15577470678135297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4890.1439959009755796No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4750.1398733189435589No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4270.12573875197663087No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4270.12573875197663087No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4240.12485534154119783No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA4020.11837699834802248No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA3980.11719911776744514No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3880.11425441631600179No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3710.1092484238485481No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.10630372239710476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3600.10600925225196042No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3590.10571478210681609No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA3550.10453690152623876No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.10453690152623876No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3460.10188667021993976No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA3440.10129772992965107No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA3440.10129772992965107No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC3430.10100325978450674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAG251.2317944E-437.034
TATTGAC352.383391E-531.71428530
TGATCGC303.594186E-430.8333329
TGCTCAA303.594186E-430.83333231
TGACTGT303.594186E-430.83333233
AGTCCTA250.00549095829.629
AAATAGG250.00549095829.66
TAAGTCT250.00549095829.635
GTAATCT250.00549095829.636
ATATAGA250.00549095829.61
ACTGTGA250.00549095829.68
GTATACT250.00549095829.64
TATAGAT250.00549095829.62
TATACTG250.00549095829.65
AAGTCTG250.00549095829.636
TAGGCAT453.997331E-628.7777775
GCTCAAA405.9279962E-527.75000232
TCGCAGG358.8564784E-426.4285712
TTGTGCA358.8564784E-426.4285719
GTGATCG358.8564784E-426.428578
Produced by FastQC (version 0.11.5)