FastQCFastQC Report
Fri 10 Feb 2017
ERR1630338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533020
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15910.10378207720708145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.10091192548042426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.019.8497851
GTCTTAT1457.2759576E-1219.137931
GTACTAG609.238733E-418.51
CTTATAC7500.017.75999837
GTAGAAC1603.45608E-1117.343753
CGAATTA1005.8803653E-616.65000215
TCTTATA11850.015.76793237
GTATATA1557.212293E-915.51612951
GACGGAC2900.015.3103457
CGCAAGA3650.015.2054792
AAGACGG3000.014.85
GTCCTAT2900.014.6724141
CTCTTAT19800.014.29545337
GCGAAAG3250.014.23076918
GTATCAA16400.014.1006092
CTATACA1051.6561999E-414.0952381
CCGCTTA1051.6561999E-414.09523825
AATAACG2500.014.0599992
TATACTG1458.914776E-714.0344835
GCGCAAG3500.013.7428581
Produced by FastQC (version 0.11.5)