FastQCFastQC Report
Fri 10 Feb 2017
ERR1630336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122215
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.017.7255821
TAGAGCG852.7248743E-517.4117645
CGAATTA1504.6766218E-916.03333315
TGCGTTA1353.9764564E-715.07407337
ATAATAC2450.014.3469383
ATACCGT2201.8189894E-1114.2954546
ACGATTC1304.447942E-614.2307698
CGATTCG1051.6566731E-414.0952389
TACGATT1203.3029464E-513.8750017
TCTTATA11900.013.83613537
TAAACCG1356.572771E-613.7037035
ATAGTAC2601.8189894E-1213.5192313
CTTATAC7550.013.23178937
AATGCGT1551.8894643E-613.12903235
TAGTACC1551.8894643E-613.1290324
CGATAGA850.00940815813.0588248
CCGAATT1857.391645E-813.014
CTAGTAC1000.001833368812.9500013
TATACTG2900.012.758625
TATAGTA3200.012.7187512
Produced by FastQC (version 0.11.5)