FastQCFastQC Report
Fri 10 Feb 2017
ERR1630335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772301
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG77340.2789740363690667No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT55670.2008079209292209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.18742553568317435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47670.17195102551995617No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG44430.16026398287920393No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC43930.1584604269161249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42380.15286940343057986No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA40900.1475308777798659No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC37270.134437061487912No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC35860.12935103367202913No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA34190.1233271567553451No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC34140.1231468011590372No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC33950.12246144989306718No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA33700.12155967191152764No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT32780.11824112893946219No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT32690.11791648886610798No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA31240.11268617657317875No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT30720.11081047837157654No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA29320.1057605216749552No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG29200.10532766824381623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.023.4575161
ACCGTTC656.9066264E-519.9230778
CGAATTA3200.019.07812515
CGGCCTT11300.018.17256724
TTCCTCG11500.017.85652219
TCTCGCC11700.017.07692112
ATCTCGC12150.016.74897211
TCCTCGG14050.016.59074820
TACGACG1452.9849616E-916.5862065
CGCGCTT1751.3278623E-1015.85714212
ATGCGTT2450.015.85714236
CCGAATT4000.015.72514
TATACTG2850.015.5789475
TCGGCCT13550.015.56457523
GGCCTTT13350.015.52059925
AATGCGT2550.015.23529535
CTCGCCT13150.015.19391613
TACCGTC1001.0942486E-414.87
TCGCCAT10200.014.69117713
GTTGACG2201.8189894E-1114.29545436
Produced by FastQC (version 0.11.5)