##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630335.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2772301
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93580927900686	33.0	31.0	34.0	30.0	34.0
2	32.151000198030445	33.0	31.0	34.0	30.0	34.0
3	32.2753575459519	34.0	31.0	34.0	30.0	34.0
4	35.89583346108521	37.0	35.0	37.0	35.0	37.0
5	35.82021144168689	37.0	35.0	37.0	35.0	37.0
6	35.871937426707994	37.0	35.0	37.0	35.0	37.0
7	35.85471454939417	37.0	35.0	37.0	35.0	37.0
8	35.848757404048115	37.0	35.0	37.0	35.0	37.0
9	37.60592193993365	39.0	37.0	39.0	35.0	39.0
10	37.5047824893473	39.0	37.0	39.0	35.0	39.0
11	37.56847037893793	39.0	37.0	39.0	35.0	39.0
12	37.505703024310854	39.0	37.0	39.0	35.0	39.0
13	37.50163780917007	39.0	37.0	39.0	35.0	39.0
14	38.77960726486771	40.0	38.0	41.0	35.0	41.0
15	38.80438199171014	40.0	38.0	41.0	35.0	41.0
16	38.758567341713615	40.0	38.0	41.0	35.0	41.0
17	38.73363606621359	40.0	38.0	41.0	35.0	41.0
18	38.68704083719625	40.0	38.0	41.0	34.0	41.0
19	38.736735657491735	40.0	38.0	41.0	34.0	41.0
20	38.71455300127944	40.0	38.0	41.0	34.0	41.0
21	38.658038214465165	40.0	38.0	41.0	34.0	41.0
22	38.63748525142111	40.0	38.0	41.0	34.0	41.0
23	38.59487912748291	40.0	38.0	41.0	34.0	41.0
24	38.572793502581426	40.0	38.0	41.0	34.0	41.0
25	38.550999332323585	40.0	38.0	41.0	34.0	41.0
26	38.412198026116215	40.0	38.0	41.0	34.0	41.0
27	38.30149864679196	40.0	38.0	41.0	34.0	41.0
28	38.26561004739384	40.0	38.0	41.0	34.0	41.0
29	38.227656376418004	40.0	38.0	41.0	34.0	41.0
30	38.17527606129349	40.0	38.0	41.0	33.0	41.0
31	38.1279161245478	40.0	38.0	41.0	33.0	41.0
32	38.04614181504822	40.0	38.0	41.0	33.0	41.0
33	37.98782527582683	40.0	38.0	41.0	33.0	41.0
34	37.93965770672088	40.0	37.0	41.0	33.0	41.0
35	37.840944399616056	40.0	37.0	41.0	33.0	41.0
36	37.76562609904192	40.0	37.0	41.0	33.0	41.0
37	37.72068184515318	40.0	37.0	41.0	33.0	41.0
38	37.626193548247464	40.0	37.0	41.0	32.0	41.0
39	37.559544580476654	40.0	37.0	41.0	32.0	41.0
40	37.48790950189031	40.0	37.0	41.0	32.0	41.0
41	37.38422559455124	40.0	37.0	41.0	31.0	41.0
42	37.37819378198832	40.0	37.0	41.0	31.0	41.0
43	36.20837239534956	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	8.0
11	5.0
12	6.0
13	1.0
14	8.0
15	5.0
16	20.0
17	47.0
18	74.0
19	158.0
20	332.0
21	636.0
22	1125.0
23	2128.0
24	3545.0
25	5826.0
26	8693.0
27	13357.0
28	19507.0
29	27446.0
30	37718.0
31	50253.0
32	65141.0
33	84691.0
34	111695.0
35	151119.0
36	212207.0
37	325541.0
38	632435.0
39	1018569.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.16684984783398	19.283800712837458	12.501095660247572	26.048253779080987
2	17.153115769175137	21.65562108876345	36.31358932525725	24.877673816804162
3	18.796552033852024	23.72538191199296	31.523272545080783	25.954793509074232
4	14.341227738257858	15.907327523237916	35.794778416917936	33.95666632158629
5	13.464915966917012	37.650096436137346	35.275173943954854	13.609813652990782
6	33.158484594566026	36.17049519514656	16.558014443597575	14.113005766689838
7	28.019937229038263	31.83398916640004	21.98808859499744	18.157985009564257
8	25.94231290180972	35.60453933393236	20.723615509282723	17.7295322549752
9	26.19098719799906	15.150699725606994	19.73970358918458	38.91860948720936
10	15.673839168257702	28.038441713219452	34.2670583028322	22.020660815690647
11	34.08013776281869	22.5635672316967	22.779957876146923	20.576337129337688
12	20.349521931420867	26.17746052827597	30.451383165103646	23.02163437519952
13	28.744533872764897	20.469891256396764	26.421481650080565	24.364093220757773
14	21.98538326105282	21.397063305896438	25.787676013535325	30.829877419515412
15	24.11394000867871	28.521470071251283	23.423214145938697	23.941375774131306
16	24.145105455720717	27.82850779911705	24.15426752001316	23.87211922514907
17	23.184206909711463	27.67322163069594	25.903752875319093	23.238818584273496
18	21.972722298192007	26.689706492909682	27.52089329405429	23.81667791484402
19	23.81548756790839	26.613848929102573	27.50816018895495	22.062503314034082
20	24.09475017323155	25.719609811488723	27.505166286056237	22.680473729223486
21	23.02823539002439	26.990070703000864	26.131902704648592	23.849791202326152
22	23.380938794164123	26.37426455496715	26.416070982191325	23.828725668677393
23	22.798354146970333	26.930481213980734	26.64256154003479	23.62860309901414
24	24.114012150917237	26.647936136804766	26.586254522867463	22.65179718941053
25	24.061709027987945	25.82609175554891	26.748502417306057	23.36369679915709
26	23.84394768100578	26.374985977352388	26.41993059195232	23.361135749689517
27	23.74446353408234	26.02780145445967	26.25555450147729	23.9721805099807
28	22.925216273413312	26.49023320339314	26.93967934939244	23.644871173801114
29	23.098249432511118	26.96312557691246	26.668677030380177	23.26994796019624
30	22.701972116303388	27.736165733807404	26.513895857628732	23.047966292260472
31	23.579221736745033	26.5377749385799	26.76931545311999	23.113687871555072
32	22.68397262779186	26.873236347712602	26.822808922984915	23.619982101510622
33	22.989350723460404	26.460041676571194	26.97924936722239	23.57135823274601
34	23.27975930463539	26.363082507996065	26.82720959953483	23.529948587833715
35	23.619909959272096	26.46087131231421	26.805711212454923	23.113507515958766
36	23.216490561450577	26.761596233598013	26.749656693122425	23.27225651182898
37	24.234056835819775	25.322863570730597	26.568507532190765	23.87457206125886
38	23.493697112975827	26.1070136323581	27.417405252892813	22.981884001773256
39	23.43353048604751	25.97167479288865	27.40110110698658	23.19369361407726
40	23.009983403678028	25.236365026741325	28.159604602819105	23.594046966761546
41	22.405251089257625	26.059183328217244	28.25555378005491	23.28001180247022
42	23.210610969010943	25.037108163940353	27.891560115586294	23.860720751462413
43	22.652230042841666	25.184530828362433	28.123389199080474	24.039849929715423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	344.0
1	352.5
2	361.0
3	711.5
4	1062.0
5	1062.0
6	1401.5
7	1741.0
8	1623.5
9	1506.0
10	2464.5
11	3423.0
12	3423.0
13	5259.5
14	7096.0
15	11716.5
16	16337.0
17	17514.0
18	18691.0
19	18691.0
20	21795.5
21	24900.0
22	30088.0
23	35276.0
24	40117.0
25	44958.0
26	44958.0
27	50157.0
28	55356.0
29	71999.0
30	88642.0
31	93887.5
32	99133.0
33	99133.0
34	107993.0
35	116853.0
36	123281.0
37	129709.0
38	146838.5
39	163968.0
40	163968.0
41	177409.5
42	190851.0
43	177324.0
44	163797.0
45	176465.0
46	189133.0
47	189133.0
48	195404.5
49	201676.0
50	210206.0
51	218736.0
52	230692.5
53	242649.0
54	242649.0
55	208592.0
56	174535.0
57	161475.5
58	148416.0
59	129805.5
60	111195.0
61	111195.0
62	103443.5
63	95692.0
64	84815.0
65	73938.0
66	63656.5
67	53375.0
68	53375.0
69	44808.0
70	36241.0
71	31717.0
72	27193.0
73	21667.0
74	16141.0
75	16141.0
76	12193.0
77	8245.0
78	7044.0
79	5843.0
80	4212.0
81	2581.0
82	2581.0
83	1866.5
84	1152.0
85	940.0
86	728.0
87	593.0
88	458.0
89	458.0
90	369.0
91	280.0
92	169.5
93	59.0
94	39.5
95	20.0
96	20.0
97	15.5
98	11.0
99	5.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2772301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.641504924742065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.400409347902	37.60878689053031
2	10.323536307805616	9.42363466466927
3	2.9618667388355298	4.055521660409746
4	1.2583234144543856	2.2972709727095237
5	0.6853357757219803	1.5639878091358348
6	0.42589134654349575	1.1662993194401994
7	0.26762104498579137	0.8550239069878529
8	0.20769094335258229	0.7583461771080968
9	0.15784295618315325	0.6483771055772313
>10	1.0227250491773414	9.161074480298396
>50	0.12887804303214248	4.164904490053736
>100	0.12690064918178703	12.016047202242246
>500	0.02219698843934268	7.069113772565512
>1k	0.010543569508687614	8.542347837709626
>5k	2.3782487613581082E-4	0.6692637105624711
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	7734	0.2789740363690667	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	5567	0.2008079209292209	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5196	0.18742553568317435	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4767	0.17195102551995617	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	4443	0.16026398287920393	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	4393	0.1584604269161249	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4238	0.15286940343057986	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	4090	0.1475308777798659	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	3727	0.134437061487912	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	3586	0.12935103367202913	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	3419	0.1233271567553451	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	3414	0.1231468011590372	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	3395	0.12246144989306718	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	3370	0.12155967191152764	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	3278	0.11824112893946219	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	3269	0.11791648886610798	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	3124	0.11268617657317875	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	3072	0.11081047837157654	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	2932	0.1057605216749552	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	2920	0.10532766824381623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.214223852316181E-5	0.0
10	7.214223852316181E-5	0.0	0.0	1.0821335778474271E-4	0.0
11	2.1642671556948542E-4	0.0	0.0	1.8035559630790452E-4	0.0
12	2.1642671556948542E-4	0.0	0.0	2.1642671556948542E-4	0.0
13	2.1642671556948542E-4	0.0	0.0	2.1642671556948542E-4	0.0
14	2.1642671556948542E-4	0.0	0.0	2.1642671556948542E-4	0.0
15	2.1642671556948542E-4	0.0	0.0	2.5249783483106633E-4	0.0
16	2.1642671556948542E-4	0.0	0.0	3.2464007335422814E-4	0.0
17	2.1642671556948542E-4	0.0	0.0	5.771379081852945E-4	0.0
18	2.8856895409264723E-4	0.0	0.0	6.132090274468754E-4	0.0
19	2.8856895409264723E-4	0.0	0.0	7.214223852316181E-4	0.0
20	2.8856895409264723E-4	0.0	0.0	7.57493504493199E-4	0.0
21	3.2464007335422814E-4	0.0	0.0	8.657068622779417E-4	0.0
22	3.6071119261580904E-4	0.0	0.0	0.0011903469356321697	0.0
23	3.6071119261580904E-4	0.0	0.0	0.0015871292475095598	0.0
24	3.9678231187738994E-4	0.0	0.0	0.002524978348310663	0.0
25	3.9678231187738994E-4	0.0	0.0	0.003246400733542281	0.0
26	3.9678231187738994E-4	0.0	0.0	0.004039965357297061	0.0
27	3.9678231187738994E-4	0.0	0.0	0.011001691374782175	0.0
28	3.9678231187738994E-4	0.0	0.0	0.03221150950059175	0.0
29	3.9678231187738994E-4	0.0	0.0	0.06142911610247228	0.0
30	4.3285343113897085E-4	0.0	0.0	0.09692309745586788	0.0
31	4.3285343113897085E-4	0.0	0.0	0.21444280401009846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1530	0.0	23.457516	1
ACCGTTC	65	6.9066264E-5	19.923077	8
CGAATTA	320	0.0	19.078125	15
CGGCCTT	1130	0.0	18.172567	24
TTCCTCG	1150	0.0	17.856522	19
TCTCGCC	1170	0.0	17.076921	12
ATCTCGC	1215	0.0	16.748972	11
TCCTCGG	1405	0.0	16.590748	20
TACGACG	145	2.9849616E-9	16.586206	5
CGCGCTT	175	1.3278623E-10	15.857142	12
ATGCGTT	245	0.0	15.857142	36
CCGAATT	400	0.0	15.725	14
TATACTG	285	0.0	15.578947	5
TCGGCCT	1355	0.0	15.564575	23
GGCCTTT	1335	0.0	15.520599	25
AATGCGT	255	0.0	15.235295	35
CTCGCCT	1315	0.0	15.193916	13
TACCGTC	100	1.0942486E-4	14.8	7
TCGCCAT	1020	0.0	14.691177	13
GTTGACG	220	1.8189894E-11	14.295454	36
>>END_MODULE