FastQCFastQC Report
Fri 10 Feb 2017
ERR1630334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100037
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89990.42851625947542826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85720.4081832843897512No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC83890.3994691522101753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65050.30975644714831213No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT55190.26280489343759184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.2467099389201238No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG38470.1831872486056198No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT35620.16961605914562458No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG33340.1587591075776284No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG29680.14133084321847664No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT29510.14052133367174008No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG28830.13728329548479384No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT28660.13647378593805728No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG27020.1286643997224811No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT26020.12390257885932486No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC25920.12342639677300925No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG25900.12333116035574611No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT25420.12104548634143113No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT25380.12085501350690486No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG25140.11971217649974737No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC23570.1122361177445921No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG22950.10928378880943526No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG22940.1092361706008037No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT21270.10128392975933281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC400.001931716223.1258
CGACACG759.271762E-619.73333236
CGAATTA2250.018.91111215
ACGACAC902.1539418E-618.535
CAGGACA22000.016.734094
CCAGGAC23600.015.7563573
TGCATCA21300.015.63380214
ATCGTAC957.0641196E-515.57894825
CGATGCG1557.21775E-915.51612933
GCGTTAT2150.015.4883721
GACAGGC22600.015.389387
CGTTATT2055.456968E-1215.3414632
GCTGCAT22700.015.07709212
GCATCAG22600.014.89823115
TCCAGGA24900.014.8594382
CGAGCTA750.0041059114.79999922
CGTCGTA1752.240995E-914.79999910
CCGAATT3000.014.79999914
CGTTTGA750.0041059114.79999922
ACGTTAA1904.5656634E-1014.60526420
Produced by FastQC (version 0.11.5)