##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2100037
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84004043738277	33.0	31.0	34.0	30.0	34.0
2	32.05196479871545	33.0	31.0	34.0	30.0	34.0
3	32.158219593273834	34.0	31.0	34.0	30.0	34.0
4	35.80110540909517	37.0	35.0	37.0	35.0	37.0
5	35.73502609715924	37.0	35.0	37.0	35.0	37.0
6	35.789137524719806	37.0	35.0	37.0	35.0	37.0
7	35.765231279258415	37.0	35.0	37.0	33.0	37.0
8	35.77246877078832	37.0	35.0	37.0	35.0	37.0
9	37.515325206174936	39.0	37.0	39.0	35.0	39.0
10	37.4090446977839	39.0	37.0	39.0	34.0	39.0
11	37.48204245925191	39.0	37.0	39.0	35.0	39.0
12	37.41666313498286	39.0	37.0	39.0	34.0	39.0
13	37.41328033744168	39.0	37.0	39.0	34.0	39.0
14	38.64774192073759	40.0	38.0	41.0	34.0	41.0
15	38.656572241346225	40.0	38.0	41.0	34.0	41.0
16	38.614690598308506	40.0	38.0	41.0	34.0	41.0
17	38.59760756596193	40.0	38.0	41.0	34.0	41.0
18	38.4980007495106	40.0	38.0	41.0	34.0	41.0
19	38.53850765486513	40.0	38.0	41.0	34.0	41.0
20	38.51962465423228	40.0	38.0	41.0	34.0	41.0
21	38.47648969994338	40.0	38.0	41.0	34.0	41.0
22	38.42221113247052	40.0	38.0	41.0	34.0	41.0
23	38.35762274664685	40.0	38.0	41.0	34.0	41.0
24	38.30937264438674	40.0	38.0	41.0	34.0	41.0
25	38.28960442125543	40.0	38.0	41.0	34.0	41.0
26	38.09367406383792	40.0	38.0	41.0	33.0	41.0
27	37.97427188187637	40.0	37.0	41.0	33.0	41.0
28	37.878756898092746	40.0	37.0	41.0	33.0	41.0
29	37.799648768093135	40.0	37.0	41.0	33.0	41.0
30	37.73027903794076	40.0	37.0	41.0	33.0	41.0
31	37.67407288538249	40.0	37.0	41.0	33.0	41.0
32	37.570659945515246	40.0	37.0	41.0	32.0	41.0
33	37.4570476615412	40.0	36.0	41.0	32.0	41.0
34	37.3638183517719	40.0	36.0	41.0	32.0	41.0
35	37.20631207926336	40.0	36.0	41.0	31.0	41.0
36	37.11309276931787	39.0	36.0	41.0	31.0	41.0
37	37.01199788384681	39.0	36.0	41.0	31.0	41.0
38	36.898927495086994	39.0	35.0	41.0	30.0	41.0
39	36.79426267251482	39.0	35.0	41.0	30.0	41.0
40	36.65118138394705	39.0	35.0	41.0	30.0	41.0
41	36.49582507355823	39.0	35.0	41.0	30.0	41.0
42	36.44397312999723	39.0	35.0	41.0	30.0	41.0
43	35.22418843096574	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	10.0
12	6.0
13	2.0
14	3.0
15	7.0
16	26.0
17	46.0
18	108.0
19	207.0
20	400.0
21	799.0
22	1595.0
23	2630.0
24	4194.0
25	6577.0
26	9736.0
27	14104.0
28	19554.0
29	26281.0
30	35101.0
31	44670.0
32	57310.0
33	74127.0
34	97970.0
35	129608.0
36	174841.0
37	264805.0
38	469339.0
39	665971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.842054687607884	18.86981038905505	12.25326029969948	26.034874623637585
2	19.038569320445305	20.797443092669322	34.45182156314389	25.712166023741485
3	20.41216416663135	23.120306927925554	29.57057423273971	26.896954672703387
4	15.111495654600372	15.4073952030369	33.83630859837232	35.64480054399042
5	15.107543343283952	36.629068916404805	32.864040014533074	15.399347725778165
6	34.87743311189279	33.89506946782366	15.887577218877572	15.339920201405977
7	29.208675847139837	30.385321782425738	21.431908104476253	18.974094265958172
8	27.77041547363213	32.935467327480424	19.90593499066921	19.388182208218236
9	27.38661271206174	13.875707904194067	19.874364118346485	38.86331526539771
10	18.169965576796983	26.577150783533813	31.96977005643234	23.283113583236865
11	35.178999227156474	22.05946847603161	21.21843567518096	21.54309662163095
12	22.507651055671875	25.32607758815678	28.77835009573641	23.387921260434936
13	29.771761164208062	19.430181468231275	25.359791279867927	25.43826608769274
14	22.76102754380042	20.848489812322356	25.72059444666927	30.669888197207957
15	25.676690458310976	26.937906332126527	22.522888882433975	24.862514327128522
16	24.48247340404002	25.946590464834667	23.872150824009292	25.69878530711602
17	23.292780079589072	26.654625608977366	26.050541014277368	24.00205329715619
18	23.21916232904468	24.57104327209473	27.13575998899067	25.07403440986992
19	24.246906125939685	24.887894832329145	26.869764675574764	23.995434366156406
20	24.10567051913847	25.276078469093637	26.755957156945332	23.862293854822557
21	26.22163323789057	24.658422684933644	25.682214170512236	23.43772990666355
22	25.245507579152175	25.22636505928229	26.005398952494645	23.522728409070886
23	24.550234114922738	25.389076478176335	25.744070223524634	24.316619183376293
24	24.59542379491409	25.311649270941416	26.155491546101327	23.937435388043163
25	24.47756872855097	24.900656512242403	26.5291516292332	24.092623129973425
26	24.898561311062615	25.530502557812078	26.411058471826927	23.159877659298385
27	25.267078627662276	25.147175978328	25.49616982938872	24.089575564621004
28	23.899436057555175	24.736992729175725	27.137426626292775	24.226144586976325
29	23.82515165208994	24.909275408004717	27.319756747143025	23.94581619276232
30	23.641154893937582	25.51445522150324	26.872050349589077	23.972339534970097
31	23.65624986607379	25.548407004257545	26.552579787879928	24.242763341788738
32	23.29782760970402	25.359457952407503	26.907240205767803	24.435474232120672
33	24.121241673360995	25.476789218475677	26.54315138257088	23.858817725592456
34	24.48418765955076	25.61216778561521	26.30334608390233	23.6002984709317
35	24.50890150983054	25.33436315645867	26.90409740399812	23.252637929712666
36	24.183716763085602	25.767022200085044	25.50797914512935	24.541281891700002
37	23.333446029760427	24.999702386196052	26.885573920840443	24.781277663203078
38	24.042385919867126	24.85875248864663	27.320756729524287	23.778104861961957
39	22.81650275685619	25.222841311843553	27.513896183733905	24.44675974756635
40	23.572679909925395	24.133384316562044	28.46040331670347	23.833532456809095
41	22.18827573037999	24.531472540721904	28.802730618555767	24.477521110342344
42	22.537983854570182	25.08579610740192	27.779224842228974	24.596995195798932
43	22.24194145150776	23.47287214463364	29.091296962863034	25.193889440995566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1674.0
1	1265.5
2	857.0
3	1624.5
4	2392.0
5	2392.0
6	3150.5
7	3909.0
8	3537.0
9	3165.0
10	4334.0
11	5503.0
12	5503.0
13	9662.0
14	13821.0
15	18280.0
16	22739.0
17	20684.5
18	18630.0
19	18630.0
20	22707.0
21	26784.0
22	25302.5
23	23821.0
24	27795.0
25	31769.0
26	31769.0
27	35400.0
28	39031.0
29	44022.0
30	49013.0
31	55998.0
32	62983.0
33	62983.0
34	70601.5
35	78220.0
36	86643.0
37	95066.0
38	98567.0
39	102068.0
40	102068.0
41	108234.5
42	114401.0
43	115867.0
44	117333.0
45	118422.5
46	119512.0
47	119512.0
48	119714.0
49	119916.0
50	124355.0
51	128794.0
52	136217.5
53	143641.0
54	143641.0
55	144381.5
56	145122.0
57	138563.5
58	132005.0
59	118277.0
60	104549.0
61	104549.0
62	100456.0
63	96363.0
64	84252.5
65	72142.0
66	61049.0
67	49956.0
68	49956.0
69	43692.5
70	37429.0
71	34919.0
72	32409.0
73	36640.5
74	40872.0
75	40872.0
76	38599.5
77	36327.0
78	27813.5
79	19300.0
80	11966.5
81	4633.0
82	4633.0
83	3156.5
84	1680.0
85	1318.0
86	956.0
87	811.0
88	666.0
89	666.0
90	560.5
91	455.0
92	266.5
93	78.0
94	60.0
95	42.0
96	42.0
97	24.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2100037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.30389993800722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06806467235877	38.348356194291576
2	11.116014907300215	10.51661713768652
3	3.2452647492620166	4.605410369142977
4	1.334645876933057	2.525358200604609
5	0.7042470070228777	1.6656814975925638
6	0.445273056596861	1.2637891268609123
7	0.2933328443391239	0.9713051262004274
8	0.21273421472880766	0.8050526405537648
9	0.16836391239975262	0.7167842698796381
>10	1.1353412851757114	10.62213296785988
>50	0.14534046672884315	4.784859793294154
>100	0.10774885117261822	10.034036887533853
>500	0.015348203885372785	4.966665468246869
>1k	0.0076741019426863925	6.112168697293487
>5k	6.058501533699784E-4	2.0617816229587724
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8999	0.42851625947542826	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8572	0.4081832843897512	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	8389	0.3994691522101753	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6505	0.30975644714831213	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	5519	0.26280489343759184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5181	0.2467099389201238	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3847	0.1831872486056198	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3562	0.16961605914562458	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	3334	0.1587591075776284	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2968	0.14133084321847664	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2951	0.14052133367174008	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2883	0.13728329548479384	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2866	0.13647378593805728	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	2702	0.1286643997224811	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	2602	0.12390257885932486	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2592	0.12342639677300925	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2590	0.12333116035574611	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2542	0.12104548634143113	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2538	0.12085501350690486	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2514	0.11971217649974737	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2357	0.1122361177445921	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	2295	0.10928378880943526	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	2294	0.1092361706008037	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	2127	0.10128392975933281	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.7618208631562205E-5	0.0	0.0	0.0	0.0
6	4.7618208631562205E-5	0.0	0.0	0.0	0.0
7	4.7618208631562205E-5	0.0	0.0	0.0	0.0
8	4.7618208631562205E-5	0.0	0.0	0.0	0.0
9	4.7618208631562205E-5	0.0	0.0	0.0	0.0
10	4.7618208631562205E-5	0.0	0.0	1.4285462589468662E-4	0.0
11	4.7618208631562205E-5	0.0	0.0	3.8094566905249764E-4	0.0
12	4.7618208631562205E-5	0.0	0.0	4.7618208631562204E-4	0.0
13	4.7618208631562205E-5	0.0	0.0	6.666549208418709E-4	0.0
14	4.7618208631562205E-5	0.0	0.0	8.095095467365575E-4	0.0
15	4.7618208631562205E-5	0.0	0.0	0.0010952187985259307	0.0
16	4.7618208631562205E-5	0.0	0.0	0.0018571101366309261	0.0
17	9.523641726312441E-5	0.0	0.0	0.0026666196833674835	0.0
18	9.523641726312441E-5	0.0	0.0	0.002999947143788419	0.0
19	9.523641726312441E-5	0.0	0.0	0.003761838481893414	0.0
20	9.523641726312441E-5	0.0	0.0	0.004476111611366847	1.9047283452624882E-4
21	9.523641726312441E-5	0.0	0.0	0.005666566827155902	1.9047283452624882E-4
22	9.523641726312441E-5	0.0	0.0	0.008142713675997138	1.9047283452624882E-4
23	9.523641726312441E-5	0.0	0.0	0.011761697531995866	1.9047283452624882E-4
24	9.523641726312441E-5	0.0	0.0	0.018237773905888325	1.9047283452624882E-4
25	9.523641726312441E-5	0.0	0.0	0.02180913955325549	1.9047283452624882E-4
26	9.523641726312441E-5	0.0	0.0	0.030047089646515753	1.9047283452624882E-4
27	9.523641726312441E-5	0.0	0.0	0.05866563303408464	1.9047283452624882E-4
28	9.523641726312441E-5	0.0	0.0	0.11590271980922241	1.9047283452624882E-4
29	9.523641726312441E-5	0.0	0.0	0.18904428826730196	1.9047283452624882E-4
30	9.523641726312441E-5	0.0	0.0	0.3022803883931569	1.9047283452624882E-4
31	9.523641726312441E-5	0.0	0.0	0.5315144447454974	1.9047283452624882E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATC	40	0.0019317162	23.125	8
CGACACG	75	9.271762E-6	19.733332	36
CGAATTA	225	0.0	18.911112	15
ACGACAC	90	2.1539418E-6	18.5	35
CAGGACA	2200	0.0	16.73409	4
CCAGGAC	2360	0.0	15.756357	3
TGCATCA	2130	0.0	15.633802	14
ATCGTAC	95	7.0641196E-5	15.578948	25
CGATGCG	155	7.21775E-9	15.516129	33
GCGTTAT	215	0.0	15.488372	1
GACAGGC	2260	0.0	15.38938	7
CGTTATT	205	5.456968E-12	15.341463	2
GCTGCAT	2270	0.0	15.077092	12
GCATCAG	2260	0.0	14.898231	15
TCCAGGA	2490	0.0	14.859438	2
CGAGCTA	75	0.00410591	14.799999	22
CGTCGTA	175	2.240995E-9	14.799999	10
CCGAATT	300	0.0	14.799999	14
CGTTTGA	75	0.00410591	14.799999	22
ACGTTAA	190	4.5656634E-10	14.605264	20
>>END_MODULE