FastQCFastQC Report
Fri 10 Feb 2017
ERR1630333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786095
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104620.3755076549794605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89640.3217406441632464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73450.2636306371462567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49260.17680660566132886No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG38100.1367505415285552No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT32200.11557394848345084No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA29520.1059547502866916No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA29330.10527279220557806No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC28410.10197067939176517No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG28150.1010374735965572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.021.4392531
TCGCGTT450.003827154620.55555713
AAGACGG6650.019.751885
TATACCG759.2735445E-619.7333345
CAAGACG7050.019.4184424
GACGGAC7050.018.3687957
ACGGACC7250.018.117248
CGGACCA7500.017.7600029
AACACCG852.7253702E-517.4117645
CTTATAC15650.017.14057537
GACGTTA650.001580697517.07692315
CGCAAGA7650.016.9281042
CTTACAC3000.016.653
TCTAGCG3750.016.2828
GCGCAAG8250.015.696971
AGACGGA8500.015.67058856
GCGAAAG8400.015.63690618
CGAAAGC8600.015.27325519
CTAGCGG4000.015.26250129
TCGCCAT9100.014.84065913
Produced by FastQC (version 0.11.5)