##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2786095
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.906031560302143	33.0	31.0	34.0	30.0	34.0
2	32.11654986639005	33.0	31.0	34.0	30.0	34.0
3	32.232454385080196	34.0	31.0	34.0	30.0	34.0
4	35.85840324899187	37.0	35.0	37.0	35.0	37.0
5	35.78297042993868	37.0	35.0	37.0	35.0	37.0
6	35.84349241501097	37.0	35.0	37.0	35.0	37.0
7	35.82170098291695	37.0	35.0	37.0	35.0	37.0
8	35.82924810532304	37.0	35.0	37.0	35.0	37.0
9	37.57142703317726	39.0	37.0	39.0	35.0	39.0
10	37.47888747512199	39.0	37.0	39.0	35.0	39.0
11	37.54048838966367	39.0	37.0	39.0	35.0	39.0
12	37.475170444654616	39.0	37.0	39.0	35.0	39.0
13	37.4802072434716	39.0	37.0	39.0	35.0	39.0
14	38.72423373933768	40.0	38.0	41.0	35.0	41.0
15	38.735095536943284	40.0	38.0	41.0	35.0	41.0
16	38.682392739658916	40.0	38.0	41.0	34.0	41.0
17	38.669148036947774	40.0	38.0	41.0	34.0	41.0
18	38.62782783788779	40.0	38.0	41.0	34.0	41.0
19	38.66907876436374	40.0	38.0	41.0	34.0	41.0
20	38.65457423382907	40.0	38.0	41.0	34.0	41.0
21	38.59551522830341	40.0	38.0	41.0	34.0	41.0
22	38.55860981050539	40.0	38.0	41.0	34.0	41.0
23	38.49899482968097	40.0	38.0	41.0	34.0	41.0
24	38.46313783270133	40.0	38.0	41.0	34.0	41.0
25	38.44114073640705	40.0	38.0	41.0	34.0	41.0
26	38.282273217532065	40.0	38.0	41.0	34.0	41.0
27	38.15608441205343	40.0	38.0	41.0	33.0	41.0
28	38.08739364594531	40.0	38.0	41.0	33.0	41.0
29	38.03431290031388	40.0	38.0	41.0	33.0	41.0
30	37.96901433727134	40.0	37.0	41.0	33.0	41.0
31	37.90505420669432	40.0	37.0	41.0	33.0	41.0
32	37.81067982247554	40.0	37.0	41.0	33.0	41.0
33	37.7136684140347	40.0	37.0	41.0	33.0	41.0
34	37.65569551648454	40.0	37.0	41.0	33.0	41.0
35	37.526120250745215	40.0	37.0	41.0	32.0	41.0
36	37.431416014170374	40.0	37.0	41.0	32.0	41.0
37	37.37158172998408	40.0	37.0	41.0	32.0	41.0
38	37.25370599351422	40.0	36.0	41.0	31.0	41.0
39	37.16948309372078	40.0	36.0	41.0	31.0	41.0
40	37.057676066322216	39.0	36.0	41.0	31.0	41.0
41	36.933740593913704	39.0	36.0	41.0	31.0	41.0
42	36.879788018714365	39.0	36.0	41.0	30.0	41.0
43	35.69473941125482	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	5.0
11	7.0
12	3.0
13	5.0
14	6.0
15	12.0
16	19.0
17	60.0
18	115.0
19	255.0
20	492.0
21	886.0
22	1651.0
23	2787.0
24	4387.0
25	7097.0
26	10552.0
27	15514.0
28	22464.0
29	30967.0
30	41759.0
31	54582.0
32	70545.0
33	90940.0
34	120444.0
35	160421.0
36	224040.0
37	343414.0
38	627908.0
39	954750.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.85880237393197	20.27335751293477	13.073926050619237	24.79391406251402
2	18.277912275065997	21.854387592670026	35.50736783921582	24.360332293048156
3	19.90718191590739	23.811607285465858	30.553265412701293	25.72794538592546
4	14.87849481083739	16.10903432941088	34.59365168811544	34.41881917163629
5	14.268070543179611	37.06750846615065	34.0998063597975	14.564614630872242
6	33.51863450456642	35.184478634073855	16.279236709444582	15.017650151915134
7	27.65881996127196	31.555277189040577	21.94620068590626	18.839702163781205
8	25.921406125778194	34.34441395573374	20.837480416138	18.896699502350064
9	26.00062094077912	15.008389879024225	20.90158447576267	38.08940470443398
10	17.016110362353043	27.287655302493274	33.30338699864865	22.392847336505035
11	34.404605729524654	22.565131483312665	22.02502068307075	21.005242104091927
12	20.62295076083192	26.859277949962223	29.506208510477926	23.011562778727935
13	28.90655200199562	20.446036477578833	26.239988227249967	24.407423293175572
14	22.40849647984006	22.278027131163867	25.470703619223322	29.842772769772747
15	24.93339243636703	28.514641460538854	23.321853705634588	23.230112397459525
16	24.459934065421315	27.169748339521803	24.78996588414968	23.580351710907202
17	23.016372377826315	27.28754762490152	26.036010975935852	23.660069021336312
18	22.343172074175506	25.586205782645603	27.999511861584047	24.071110281594848
19	23.324437967836705	26.278967515465197	27.91372871348608	22.482865803212025
20	23.99523347193832	25.362308176856857	27.815419072213977	22.827039278990846
21	24.040816985781174	26.380471591959353	26.52831292543865	23.05039849682082
22	23.793302094867546	25.766386286181913	27.105393032183038	23.3349185867675
23	22.862680561861673	26.269743135104868	27.18679729154964	23.680779011483814
24	23.92420215391076	26.451215769742237	27.08791336978818	22.53666870655882
25	23.8226980774166	25.742984356240544	27.090210491745616	23.344107074597243
26	23.505515784637637	26.505162243211377	26.91774688228506	23.071575089865924
27	23.845992329766215	25.643777401703822	26.764019173789837	23.74621109474013
28	22.789280336815505	26.202695887972233	27.66567543461368	23.34234834059858
29	22.802560573131927	26.545218307344147	27.46632831974502	23.1858927997789
30	22.535950855947124	27.40430602689427	27.30233534750251	22.757407769656098
31	23.49927048431586	26.531938071027728	27.05442563875245	22.914365805903962
32	22.54119116541252	26.40240192814674	26.94951177185272	24.106895134588015
33	22.53921707623035	26.078184699373136	27.61223145657273	23.77036676782378
34	23.1828060421486	25.906977328483055	27.45093042412409	23.459286205244258
35	23.47292536686653	25.863296118761202	27.49547305457998	23.16830545979229
36	22.51046715923183	26.65605444179039	27.37867876005664	23.45479963892114
37	23.72797768920299	25.44094871136842	27.129656382858446	23.701417216570146
38	22.742691832116275	25.809995710842593	28.145414998411756	23.301897458629373
39	23.081948031204966	25.33944463487426	28.036265812903004	23.542341521017768
40	22.57087428820625	25.148029769264866	28.922811318350593	23.358284624178285
41	21.83726685558102	25.7747492458082	29.12416841493201	23.26381548367877
42	22.80679589174095	25.06917387956979	28.794423736448326	23.32960649224093
43	21.859197191768406	25.03615275143166	28.754798382682573	24.349851674117357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	646.0
1	635.5
2	625.0
3	1351.5
4	2078.0
5	2078.0
6	2813.0
7	3548.0
8	3332.5
9	3117.0
10	4633.0
11	6149.0
12	6149.0
13	10224.0
14	14299.0
15	20025.5
16	25752.0
17	25155.0
18	24558.0
19	24558.0
20	28271.0
21	31984.0
22	32992.5
23	34001.0
24	38179.5
25	42358.0
26	42358.0
27	47557.0
28	52756.0
29	68952.0
30	85148.0
31	89670.0
32	94192.0
33	94192.0
34	102040.5
35	109889.0
36	118974.0
37	128059.0
38	143919.0
39	159779.0
40	159779.0
41	171251.5
42	182724.0
43	172930.5
44	163137.0
45	172651.0
46	182165.0
47	182165.0
48	189359.0
49	196553.0
50	199559.5
51	202566.0
52	211595.5
53	220625.0
54	220625.0
55	200057.5
56	179490.0
57	169563.5
58	159637.0
59	145366.5
60	131096.0
61	131096.0
62	119033.5
63	106971.0
64	93815.0
65	80659.0
66	68472.5
67	56286.0
68	56286.0
69	47395.0
70	38504.0
71	33237.5
72	27971.0
73	22023.5
74	16076.0
75	16076.0
76	12190.5
77	8305.0
78	6896.5
79	5488.0
80	4441.0
81	3394.0
82	3394.0
83	2534.5
84	1675.0
85	1529.5
86	1384.0
87	1346.0
88	1308.0
89	1308.0
90	1136.5
91	965.0
92	545.5
93	126.0
94	79.5
95	33.0
96	33.0
97	21.5
98	10.0
99	9.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2786095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.28432056000514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2882543085996	37.96375553931038
2	11.981819199141324	11.33104359808445
3	3.450235513693361	4.894261261109727
4	1.3675048677899826	2.5864615414379597
5	0.682576356298502	1.6137579618949325
6	0.421661828706057	1.1962795845873109
7	0.27897724465929125	0.9233874625791836
8	0.20831798877530724	0.7880139647733746
9	0.16523965792421816	0.7031920459062894
>10	0.9008047565234669	8.069490615585377
>50	0.10007238533904679	3.297009819763772
>100	0.12467867789901745	13.050852713749963
>500	0.021783580076909413	6.8572079559625925
>1k	0.007845135482242827	5.761135125438173
>5k	1.5233272781054036E-4	0.5873645197152593
>10k+	7.616636390527018E-5	0.37678629010123504
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10462	0.3755076549794605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8964	0.3217406441632464	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7345	0.2636306371462567	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4926	0.17680660566132886	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3810	0.1367505415285552	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3220	0.11557394848345084	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2952	0.1059547502866916	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2933	0.10527279220557806	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2841	0.10197067939176517	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2815	0.1010374735965572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.5892530584922625E-5	0.0
10	0.0	0.0	0.0	3.5892530584922625E-5	0.0
11	0.0	0.0	0.0	7.178506116984525E-5	0.0
12	0.0	0.0	0.0	1.7946265292461314E-4	0.0
13	0.0	0.0	0.0	1.7946265292461314E-4	0.0
14	0.0	0.0	0.0	3.5892530584922627E-4	0.0
15	0.0	0.0	0.0	5.024954281889168E-4	0.0
16	0.0	0.0	0.0	0.0012562385704722918	0.0
17	0.0	0.0	0.0	0.002009981712755667	0.0
18	0.0	0.0	0.0	0.0021535518350953575	0.0
19	0.0	0.0	0.0	0.0025483696715295065	0.0
20	0.0	0.0	0.0	0.0028355099162088874	0.0
21	0.0	0.0	0.0	0.0036969306502470304	0.0
22	0.0	0.0	0.0	0.00506084681247409	0.0
23	0.0	0.0	0.0	0.0068554733417202215	0.0
24	0.0	0.0	0.0	0.009655090727344186	0.0
25	0.0	0.0	0.0	0.01148560978717524	0.0
26	0.0	0.0	0.0	0.01557735827385642	0.0
27	0.0	0.0	0.0	0.028498669284428565	0.0
28	0.0	0.0	0.0	0.06431941480818135	0.0
29	0.0	0.0	0.0	0.11331271905660073	0.0
30	0.0	0.0	0.0	0.18954845401897638	0.0
31	0.0	0.0	0.0	0.40809807275057025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3745	0.0	21.439253	1
TCGCGTT	45	0.0038271546	20.555557	13
AAGACGG	665	0.0	19.75188	5
TATACCG	75	9.2735445E-6	19.733334	5
CAAGACG	705	0.0	19.418442	4
GACGGAC	705	0.0	18.368795	7
ACGGACC	725	0.0	18.11724	8
CGGACCA	750	0.0	17.760002	9
AACACCG	85	2.7253702E-5	17.411764	5
CTTATAC	1565	0.0	17.140575	37
GACGTTA	65	0.0015806975	17.076923	15
CGCAAGA	765	0.0	16.928104	2
CTTACAC	300	0.0	16.65	3
TCTAGCG	375	0.0	16.28	28
GCGCAAG	825	0.0	15.69697	1
AGACGGA	850	0.0	15.6705885	6
GCGAAAG	840	0.0	15.636906	18
CGAAAGC	860	0.0	15.273255	19
CTAGCGG	400	0.0	15.262501	29
TCGCCAT	910	0.0	14.840659	13
>>END_MODULE