FastQCFastQC Report
Fri 10 Feb 2017
ERR1630332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645377
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62220.2352027707203926No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG54850.20734284754120114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.20662461342939023No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT50550.19108807553705956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45880.17343463710465465No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG41330.15623482021655136No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC36530.13808995844448638No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA36000.1360864632904875No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC32140.12149497028211857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.11813061049521485No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA30730.11616491713657448No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT29870.11291396273574617No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC28040.10599623418514638No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC27400.10357691928220439No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC26800.10130881156069627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.021.9317681
TCTAGCG2500.018.528
CTAGCGG2750.016.81818229
CTTATAC12450.016.79116437
CGAGCCG4100.015.79268415
CGGCCTT10050.015.462687524
TACAGCG855.3674285E-415.2352945
GTATCAA34700.014.7680112
AAGACGG3650.014.698635
TCTCGCC10800.014.38888812
AGGTCGC10950.014.3607310
ATCTCGC10700.014.17756911
CGCAATT2353.6379788E-1214.17021212
TCGCCAT11050.014.06334913
ACTTTTG10600.013.96226430
GAGGTCG11400.013.956149
GGTCGCC11250.013.81333311
TTCCTCG10850.013.8110619
TAAACCG1356.574239E-613.7037035
ACCGTCT1356.574239E-613.7037038
Produced by FastQC (version 0.11.5)