##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630332.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2645377
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92065062938099	33.0	31.0	34.0	30.0	34.0
2	32.13477171684792	33.0	31.0	34.0	30.0	34.0
3	32.25608826265594	34.0	31.0	34.0	30.0	34.0
4	35.8760550953607	37.0	35.0	37.0	35.0	37.0
5	35.80094254996547	37.0	35.0	37.0	35.0	37.0
6	35.857399909351294	37.0	35.0	37.0	35.0	37.0
7	35.83652386786458	37.0	35.0	37.0	35.0	37.0
8	35.837776997380715	37.0	35.0	37.0	35.0	37.0
9	37.587102707856005	39.0	37.0	39.0	35.0	39.0
10	37.49392430644101	39.0	37.0	39.0	35.0	39.0
11	37.556138501241975	39.0	37.0	39.0	35.0	39.0
12	37.491057796298975	39.0	37.0	39.0	35.0	39.0
13	37.4902855056198	39.0	37.0	39.0	35.0	39.0
14	38.760207335287184	40.0	38.0	41.0	35.0	41.0
15	38.77700872125221	40.0	38.0	41.0	35.0	41.0
16	38.731352846872106	40.0	38.0	41.0	35.0	41.0
17	38.71227843895218	40.0	38.0	41.0	35.0	41.0
18	38.66549304692677	40.0	38.0	41.0	34.0	41.0
19	38.71354857927622	40.0	38.0	41.0	34.0	41.0
20	38.692359538923945	40.0	38.0	41.0	34.0	41.0
21	38.634246460901416	40.0	38.0	41.0	34.0	41.0
22	38.6083828505351	40.0	38.0	41.0	34.0	41.0
23	38.55698148127847	40.0	38.0	41.0	34.0	41.0
24	38.53006962712687	40.0	38.0	41.0	34.0	41.0
25	38.507199540935	40.0	38.0	41.0	34.0	41.0
26	38.36102869269673	40.0	38.0	41.0	34.0	41.0
27	38.248848462808894	40.0	38.0	41.0	34.0	41.0
28	38.19506709251649	40.0	38.0	41.0	34.0	41.0
29	38.15339136916969	40.0	38.0	41.0	33.0	41.0
30	38.09767719308061	40.0	38.0	41.0	33.0	41.0
31	38.04496183341732	40.0	38.0	41.0	33.0	41.0
32	37.96409472071466	40.0	37.0	41.0	33.0	41.0
33	37.888383394880954	40.0	37.0	41.0	33.0	41.0
34	37.83798452923723	40.0	37.0	41.0	33.0	41.0
35	37.728432658180665	40.0	37.0	41.0	33.0	41.0
36	37.64731945579023	40.0	37.0	41.0	33.0	41.0
37	37.59853094662878	40.0	37.0	41.0	32.0	41.0
38	37.499564712326446	40.0	37.0	41.0	32.0	41.0
39	37.42874153665054	40.0	37.0	41.0	32.0	41.0
40	37.34810199075595	40.0	37.0	41.0	31.0	41.0
41	37.23985617172902	40.0	36.0	41.0	31.0	41.0
42	37.216690475497444	40.0	36.0	41.0	31.0	41.0
43	36.05950531814558	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	8.0
11	5.0
12	6.0
13	3.0
14	6.0
15	6.0
16	15.0
17	41.0
18	90.0
19	152.0
20	370.0
21	679.0
22	1333.0
23	2192.0
24	3725.0
25	5972.0
26	9024.0
27	13343.0
28	19596.0
29	27225.0
30	36910.0
31	49439.0
32	63979.0
33	82683.0
34	110008.0
35	147024.0
36	206643.0
37	316038.0
38	599268.0
39	949590.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.52882557004162	20.136411558730572	13.091479966749542	25.243282904478264
2	17.560937439162736	21.766160362020234	36.561064831213095	24.111837367603936
3	19.455941440482775	24.0786473912792	30.836890167261604	25.628521000976423
4	14.687736379351602	16.08458832143774	35.17169764460794	34.05597765460273
5	13.797201684296795	37.424042017451576	34.7898995114874	13.98885678676423
6	33.28587947956	35.59371688798988	16.437619288290477	14.682784344159641
7	27.44897986184956	31.534938120351086	22.36686869206166	18.649213325737694
8	25.217539881839144	35.57296370233808	20.956672716213983	18.25282369960879
9	25.857977898802325	15.423510524208837	20.65187684023865	38.06663473675019
10	16.40405129401216	27.782353895115897	33.913011264557	21.900583546314948
11	33.933159621483064	22.89669865580596	22.49645324655049	20.673688476160486
12	20.105867708080925	26.811376979538267	30.013453658968082	23.069301653412726
13	28.499529556656768	20.926204469155056	26.519925137324474	24.054340836863705
14	22.028731632580158	22.223259671494837	25.67981803727786	30.068190658647143
15	24.388886725786154	28.901513848498723	23.562161461296444	23.147437964418682
16	24.125975239068005	27.795471118105286	24.65754408539879	23.421009557427922
17	22.85439844679983	27.714953293991744	26.122136844767308	23.308511414441117
18	21.927007001270518	26.407691606905175	27.95163033473112	23.713671057093187
19	23.33690056275533	26.754409673933054	27.825410140029188	22.08327962328243
20	23.61481936223079	25.887198686614422	27.994346363486187	22.5036355876686
21	23.116024672475792	27.14115984224555	26.658468717313262	23.084346767965396
22	23.13711807428582	26.400584869377784	27.06979005260876	23.392507003727637
23	22.441905255848223	27.086611851543278	27.209883506207245	23.261599386401258
24	23.886009442132444	26.98314833764715	26.888681651046337	22.24216056917407
25	23.70735815726832	26.036704787257165	27.169473386969038	23.086463668505473
26	23.415981918645244	26.82233950019222	26.848233730012772	22.913444851149762
27	23.641545231549227	26.087850616377175	26.649509691813307	23.621094460260295
28	22.608951389537292	26.66073682503477	27.44845063671454	23.281861148713396
29	22.72980372929832	27.097234156039008	27.2692701267154	22.903691987947276
30	22.394653011650135	27.723572103333478	27.105663956403948	22.776110928612443
31	23.23317243629169	26.84683506358451	27.22893561106791	22.69105688905589
32	22.362559287390795	26.721257499403677	27.21710364912071	23.699079564084816
33	22.56464768537717	26.520151948096622	27.414466822687277	23.50073354383893
34	22.885358117198418	26.457136355234056	27.402445851763286	23.255059675804244
35	23.362870396166596	26.325812918158736	27.342719015096904	22.96859767057777
36	22.75441269807668	26.92058636632888	27.266775208221738	23.058225727372697
37	23.754572599671047	25.42461811681284	27.129668096456573	23.69114118705954
38	22.956009672723397	26.072503087461634	27.941348246393616	23.030138993421353
39	22.91000488777214	25.782714524243616	28.092933445781078	23.21434714220317
40	22.671210946492693	25.207219991706285	28.86034769335335	23.261221368447675
41	21.910223004131357	26.07129343001017	28.96687315267351	23.051610413184964
42	22.822342524335852	25.11479460205483	28.58764554163735	23.475217331971965
43	21.874764920085116	25.374946557711812	28.694246604548233	24.056041917654838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	583.0
1	585.0
2	587.0
3	1112.5
4	1638.0
5	1638.0
6	2139.5
7	2641.0
8	2382.0
9	2123.0
10	3435.0
11	4747.0
12	4747.0
13	7320.0
14	9893.0
15	14220.0
16	18547.0
17	19266.0
18	19985.0
19	19985.0
20	23403.5
21	26822.0
22	30727.0
23	34632.0
24	39018.5
25	43405.0
26	43405.0
27	48698.0
28	53991.0
29	70311.0
30	86631.0
31	91356.5
32	96082.0
33	96082.0
34	104396.0
35	112710.0
36	120082.0
37	127454.0
38	145035.5
39	162617.0
40	162617.0
41	173557.0
42	184497.0
43	172882.0
44	161267.0
45	171224.0
46	181181.0
47	181181.0
48	188034.0
49	194887.0
50	200001.0
51	205115.0
52	211999.0
53	218883.0
54	218883.0
55	188710.5
56	158538.0
57	147077.5
58	135617.0
59	120208.0
60	104799.0
61	104799.0
62	95989.0
63	87179.0
64	76698.0
65	66217.0
66	57247.5
67	48278.0
68	48278.0
69	41025.0
70	33772.0
71	29255.0
72	24738.0
73	19861.5
74	14985.0
75	14985.0
76	11501.5
77	8018.0
78	6650.5
79	5283.0
80	4066.0
81	2849.0
82	2849.0
83	2154.5
84	1460.0
85	1285.0
86	1110.0
87	988.5
88	867.0
89	867.0
90	743.0
91	619.0
92	351.0
93	83.0
94	59.0
95	35.0
96	35.0
97	20.5
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2645377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.08694017336365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.02048804804045	40.40288182102936
2	9.51577882635318	9.15169374251612
3	2.5986876200124844	3.7488880833840326
4	1.0684573953582326	2.055153873935171
5	0.5895315274988179	1.4174383646573672
6	0.3658641081908085	1.0555971289291473
7	0.2655432295572092	0.8938412975211504
8	0.1948004518149574	0.7493886137736052
9	0.1488257596892729	0.6440917862190115
>10	0.9670288520415826	8.998665518658271
>50	0.11506829663897408	3.9140823536784444
>100	0.11958690731186138	12.413750541396483
>500	0.0224587235980518	7.450778825701011
>1k	0.007565043738291053	6.261446223597181
>5k	3.1521015576212717E-4	0.8423018250035895
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6222	0.2352027707203926	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5485	0.20734284754120114	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5466	0.20662461342939023	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	5055	0.19108807553705956	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4588	0.17343463710465465	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	4133	0.15623482021655136	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3653	0.13808995844448638	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3600	0.1360864632904875	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	3214	0.12149497028211857	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3125	0.11813061049521485	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	3073	0.11616491713657448	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2987	0.11291396273574617	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2804	0.10599623418514638	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2740	0.10357691928220439	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2680	0.10130881156069627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.7801795358468755E-5	0.0
5	3.7801795358468755E-5	0.0	0.0	3.7801795358468755E-5	0.0
6	3.7801795358468755E-5	0.0	0.0	3.7801795358468755E-5	0.0
7	3.7801795358468755E-5	0.0	0.0	3.7801795358468755E-5	0.0
8	3.7801795358468755E-5	0.0	0.0	3.7801795358468755E-5	0.0
9	3.7801795358468755E-5	3.7801795358468755E-5	0.0	7.560359071693751E-5	0.0
10	3.7801795358468755E-5	3.7801795358468755E-5	0.0	7.560359071693751E-5	0.0
11	3.7801795358468755E-5	3.7801795358468755E-5	0.0	1.5120718143387502E-4	0.0
12	3.7801795358468755E-5	3.7801795358468755E-5	0.0	2.2681077215081253E-4	0.0
13	3.7801795358468755E-5	3.7801795358468755E-5	0.0	2.646125675092813E-4	0.0
14	7.560359071693751E-5	3.7801795358468755E-5	0.0	4.158197489431563E-4	0.0
15	7.560359071693751E-5	3.7801795358468755E-5	0.0	5.670269303770314E-4	0.0
16	7.560359071693751E-5	3.7801795358468755E-5	0.0	7.182341118109063E-4	0.0
17	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.0010206484746786565	0.0
18	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.001096252065395594	0.0
19	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.001247459246829469	0.0
20	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.0014364682236218127	0.0
21	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.0019278915632819065	0.0
22	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.0027217292658097505	0.0
23	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.003817981331205344	0.0
24	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.0060482872573550004	0.0
25	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.0073713500949014075	0.0
26	7.560359071693751E-5	3.7801795358468755E-5	0.0	0.010168682951428094	0.0
27	7.560359071693751E-5	7.560359071693751E-5	0.0	0.02343711312225063	0.0
28	7.560359071693751E-5	7.560359071693751E-5	0.0	0.0555308373815906	0.0
29	7.560359071693751E-5	1.1340538607540627E-4	0.0	0.0984736769088111	0.0
30	7.560359071693751E-5	1.1340538607540627E-4	0.0	0.16201849490639708	0.0
31	7.560359071693751E-5	1.1340538607540627E-4	0.0	0.3516701022198348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2345	0.0	21.931768	1
TCTAGCG	250	0.0	18.5	28
CTAGCGG	275	0.0	16.818182	29
CTTATAC	1245	0.0	16.791164	37
CGAGCCG	410	0.0	15.792684	15
CGGCCTT	1005	0.0	15.4626875	24
TACAGCG	85	5.3674285E-4	15.235294	5
GTATCAA	3470	0.0	14.768011	2
AAGACGG	365	0.0	14.69863	5
TCTCGCC	1080	0.0	14.388888	12
AGGTCGC	1095	0.0	14.36073	10
ATCTCGC	1070	0.0	14.177569	11
CGCAATT	235	3.6379788E-12	14.170212	12
TCGCCAT	1105	0.0	14.063349	13
ACTTTTG	1060	0.0	13.962264	30
GAGGTCG	1140	0.0	13.95614	9
GGTCGCC	1125	0.0	13.813333	11
TTCCTCG	1085	0.0	13.81106	19
TAAACCG	135	6.574239E-6	13.703703	5
ACCGTCT	135	6.574239E-6	13.703703	8
>>END_MODULE