FastQCFastQC Report
Fri 10 Feb 2017
ERR1630331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220878
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT59660.18522899656553274No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA51500.1598942896936798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44400.13785061092037637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41510.12887790223659512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32220.10003483522194881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC11000.022.0318183
GTTCTAG11400.021.5833341
GTCTAGA9600.021.1979161
ACTGATC10500.019.380958
CTAGACA13350.018.985024
CTAGAAC10600.018.53
GGTATCA21200.018.4127371
TCTAGTT12400.018.0524228
CTCTAGT12700.017.77165427
ACGGAGT10100.017.58415826
TAGAACT12300.017.4471554
ACATGTA13150.017.0228148
GCCTCTA12650.016.96442825
CAACGGA10600.016.92924524
ACAACGG10650.016.84976623
CGGAGTC10500.016.73809427
CTAGTTT13400.016.70522329
GCAATTA11100.016.66666617
AACGGAG10800.016.61574225
GCCGTAT4900.016.2346958
Produced by FastQC (version 0.11.5)