FastQCFastQC Report
Fri 10 Feb 2017
ERR1630330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227235
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68560.30782562235237865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.24541640195129838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44880.20150545407197715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.14569634546870897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.018.560261
GGACCGT650.001580562617.0769256
GACGGAC2550.014.5098037
TACGCTA1152.2115737E-514.4782619
GCGCGTT1551.2116288E-714.32258125
CTTATAC11650.014.13304837
GTATCAA41050.013.8806332
TATACTG3400.013.6029415
GGCGCGT1857.392373E-813.024
TCTATAC2451.1459633E-1012.8367363
ATACGCT1307.0035574E-512.80769358
TCTTATA17600.012.50852337
AACGCAG46000.012.2260877
TCCGACC2002.0421612E-712.0250018
TAGTACG2002.0421612E-712.0250014
TCTAGCG1257.223368E-411.8428
CGCGTTC1901.4352845E-611.68421126
AGTACTA1901.4352845E-611.6842115
CTAGTAC1757.3406936E-611.62857153
TACGTTA1757.3406936E-611.628571519
Produced by FastQC (version 0.11.5)