##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2227235
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930947116042987	33.0	31.0	34.0	30.0	34.0
2	32.13846046779976	33.0	31.0	34.0	30.0	34.0
3	32.26486697631817	34.0	31.0	34.0	30.0	34.0
4	35.8772374715735	37.0	35.0	37.0	35.0	37.0
5	35.801174101520495	37.0	35.0	37.0	35.0	37.0
6	35.85867723881854	37.0	35.0	37.0	35.0	37.0
7	35.83701674946739	37.0	35.0	37.0	35.0	37.0
8	35.84161662330199	37.0	35.0	37.0	35.0	37.0
9	37.59346364438418	39.0	37.0	39.0	35.0	39.0
10	37.49247295413371	39.0	37.0	39.0	35.0	39.0
11	37.562536508271464	39.0	37.0	39.0	35.0	39.0
12	37.490461940477765	39.0	37.0	39.0	35.0	39.0
13	37.503915392852576	39.0	37.0	39.0	35.0	39.0
14	38.769933572344186	40.0	38.0	41.0	35.0	41.0
15	38.776555684514655	40.0	38.0	41.0	35.0	41.0
16	38.734958816649346	40.0	38.0	41.0	35.0	41.0
17	38.7061320426448	40.0	38.0	41.0	35.0	41.0
18	38.671892727978864	40.0	38.0	41.0	34.0	41.0
19	38.701991931700064	40.0	38.0	41.0	34.0	41.0
20	38.68342720907313	40.0	38.0	41.0	34.0	41.0
21	38.6338554306124	40.0	38.0	41.0	34.0	41.0
22	38.593982224596864	40.0	38.0	41.0	34.0	41.0
23	38.54542470821445	40.0	38.0	41.0	34.0	41.0
24	38.522175253172655	40.0	38.0	41.0	34.0	41.0
25	38.49919474146195	40.0	38.0	41.0	34.0	41.0
26	38.3485294546826	40.0	38.0	41.0	34.0	41.0
27	38.235603337770826	40.0	38.0	41.0	34.0	41.0
28	38.18097147359843	40.0	38.0	41.0	33.0	41.0
29	38.122922816855876	40.0	38.0	41.0	33.0	41.0
30	38.06593736179613	40.0	38.0	41.0	33.0	41.0
31	38.00767992600691	40.0	37.0	41.0	33.0	41.0
32	37.921155603247975	40.0	37.0	41.0	33.0	41.0
33	37.84135216984288	40.0	37.0	41.0	33.0	41.0
34	37.778973031584	40.0	37.0	41.0	33.0	41.0
35	37.67519278387777	40.0	37.0	41.0	33.0	41.0
36	37.59035440804406	40.0	37.0	41.0	32.0	41.0
37	37.53257379665819	40.0	37.0	41.0	32.0	41.0
38	37.43853567315528	40.0	37.0	41.0	32.0	41.0
39	37.359129593419645	40.0	37.0	41.0	32.0	41.0
40	37.261750107195695	40.0	36.0	41.0	31.0	41.0
41	37.14275188742993	40.0	36.0	41.0	31.0	41.0
42	37.101101590088156	40.0	36.0	41.0	31.0	41.0
43	35.93696264651013	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	8.0
11	4.0
12	2.0
13	3.0
14	3.0
15	7.0
16	15.0
17	18.0
18	45.0
19	133.0
20	280.0
21	503.0
22	1023.0
23	1794.0
24	3019.0
25	5059.0
26	7586.0
27	11458.0
28	16255.0
29	22959.0
30	31585.0
31	41706.0
32	54355.0
33	70628.0
34	94362.0
35	125689.0
36	177242.0
37	271758.0
38	512102.0
39	777628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34393766261755	19.662541222637035	12.441076042716642	25.552445072028775
2	18.652948611170352	21.093418521170868	34.9241548377248	25.329478029933977
3	19.985452814812984	23.23032818719174	29.66687394908934	27.117345048905932
4	14.736882277801849	15.964952059392026	34.55535675400216	34.742808908803966
5	14.864529337945928	36.24579355119689	34.08683861379693	14.802838497060256
6	34.38685185891924	34.9293181904918	15.839549935233595	14.844280015355363
7	28.68507364512501	30.460683313615313	21.823875792181784	19.03036724907789
8	26.487550707491575	34.018008876476884	20.116781569973533	19.377658846058004
9	26.322592811265988	14.477906462497222	20.3838391548265	38.815661571410295
10	16.918151878899174	26.743742802173998	33.83127510119049	22.50683021773634
11	34.985800779890766	22.14400366373553	21.824279880659205	21.045915675714507
12	20.942379228056314	25.922365623744238	30.068133807164486	23.06712134103496
13	29.889167510388443	20.531421246523156	24.89849521940882	24.680916023679586
14	22.379721942228816	21.37273345650549	25.68902697739574	30.558517623869957
15	24.934548891338363	28.21601672028322	22.625093445460404	24.224340942918012
16	24.400748012670416	26.65991689246981	24.82225719333613	24.117077901523636
17	22.9909731124017	26.756538937292202	26.267053094980998	23.9854348553251
18	23.272892173479672	25.737337999806936	27.14778638087135	23.84198344584204
19	23.683356269095988	25.900948934441136	27.156272238897106	23.25942255756577
20	23.91242953707175	25.809041255188607	26.97416303174115	23.304366175998492
21	24.178364653931894	25.735631848457842	26.609630326391244	23.476373171219024
22	24.113441105226883	25.717762158012064	26.70562378913765	23.4631729476234
23	23.85837147853729	25.80607793968755	26.75977164511154	23.57577893666362
24	23.84957132947354	26.182239413443124	26.41813728681527	23.55005197026807
25	24.14280486791919	25.76813852153006	26.662700613092017	23.426355997458735
26	23.836954789234184	26.04673507734927	26.52760036547558	23.58870976794097
27	23.789451943777824	25.859911504623444	26.49329774361484	23.85733880798389
28	23.767945457035292	25.840649953866567	26.63692874797675	23.75447584112139
29	23.396543247569294	26.102813578270815	27.10854489984218	23.392098274317707
30	23.555260221754775	26.228350398588386	26.7972620760719	23.41912730358494
31	23.80197868657775	26.026665349637558	26.7195872909684	23.451768672816296
32	23.244830473659047	26.247342557026986	26.842295491944046	23.66553147736992
33	23.21075234539687	26.05091065828258	27.13602291630654	23.602314080014008
34	23.727357014414736	25.623070758137334	26.895904563281377	23.753667664166557
35	23.512381944428853	25.817212822176376	27.19106874667469	23.479336486720083
36	23.550500957465196	25.782281618239654	26.798294746625302	23.868922677669847
37	23.463756630979667	25.310710365093943	27.225999950611406	23.999533053314984
38	23.151081946898284	25.495962482629807	27.433342238246077	23.91961333222583
39	23.056121154705274	25.286330360289778	27.848700294311108	23.808848190693844
40	23.01503882616787	25.13241754911359	28.092904430830156	23.759639193888386
41	22.60417064207414	25.124515374444094	28.31887968714572	23.95243429633604
42	22.5594964159597	25.32296771557559	28.215028948449532	23.902506920015178
43	22.398489607068857	24.957716630710276	28.320002155138546	24.323791607082324
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	538.0
1	551.5
2	565.0
3	1092.5
4	1620.0
5	1620.0
6	2034.0
7	2448.0
8	2250.0
9	2052.0
10	2894.0
11	3736.0
12	3736.0
13	6610.5
14	9485.0
15	12273.0
16	15061.0
17	15179.0
18	15297.0
19	15297.0
20	19691.0
21	24085.0
22	23756.0
23	23427.0
24	28537.0
25	33647.0
26	33647.0
27	37772.0
28	41897.0
29	48495.0
30	55093.0
31	62250.0
32	69407.0
33	69407.0
34	77813.0
35	86219.0
36	95208.5
37	104198.0
38	112343.5
39	120489.0
40	120489.0
41	129182.5
42	137876.0
43	142838.5
44	147801.0
45	150793.0
46	153785.0
47	153785.0
48	155526.5
49	157268.0
50	158068.5
51	158869.0
52	156899.0
53	154929.0
54	154929.0
55	149424.5
56	143920.0
57	137658.0
58	131396.0
59	123987.5
60	116579.0
61	116579.0
62	105774.5
63	94970.0
64	83748.0
65	72526.0
66	62320.0
67	52114.0
68	52114.0
69	44457.5
70	36801.0
71	30366.0
72	23931.0
73	19406.5
74	14882.0
75	14882.0
76	11771.5
77	8661.0
78	6865.5
79	5070.0
80	4063.5
81	3057.0
82	3057.0
83	2289.5
84	1522.0
85	1225.0
86	928.0
87	768.0
88	608.0
89	608.0
90	493.5
91	379.0
92	223.5
93	68.0
94	45.5
95	23.0
96	23.0
97	14.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2227235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.922178102048065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.58246369918058	52.591906670878075
2	9.785692596336371	12.314741847971408
3	2.717743459655838	5.130190140124228
4	1.1813816851676124	2.9734043520245677
5	0.6710092063613253	2.1110680395390617
6	0.3901223813556064	1.472840997675155
7	0.2813463560874098	1.2392047868265965
8	0.19694313791470522	0.9913672959876237
9	0.15334202618377618	0.8683752853858034
>10	0.9133247763931592	10.861149979682187
>50	0.08168466781885271	3.6047505284266292
>100	0.044088103448117304	4.6452266516358875
>500	5.004440563898715E-4	0.23616772534705666
>1k	2.1447602416707268E-4	0.40530880102416666
>5k	1.4298401611138179E-4	0.5542968974716739
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6856	0.30782562235237865	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5466	0.24541640195129838	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4488	0.20150545407197715	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3245	0.14569634546870897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.489871971300738E-5	0.0
10	0.0	0.0	0.0	4.489871971300738E-5	0.0
11	0.0	0.0	0.0	4.489871971300738E-5	0.0
12	0.0	0.0	0.0	8.979743942601476E-5	0.0
13	0.0	0.0	0.0	1.7959487885202952E-4	0.0
14	0.0	0.0	0.0	3.142910379910517E-4	0.0
15	0.0	0.0	0.0	4.938859168430812E-4	0.0
16	0.0	0.0	0.0	7.183795154081181E-4	0.0
17	0.0	0.0	0.0	0.0010326705533991699	0.0
18	0.0	0.0	0.0	0.0012571641519642067	4.489871971300738E-5
19	0.0	0.0	0.0	0.001526556470242251	4.489871971300738E-5
20	0.0	0.0	0.0	0.0016612526293812732	4.489871971300738E-5
21	0.0	0.0	0.0	0.0020653411067983398	8.979743942601476E-5
22	0.0	0.0	0.0	0.003232707819336532	8.979743942601476E-5
23	0.0	0.0	0.0	0.004938859168430812	8.979743942601476E-5
24	0.0	0.0	0.0	0.007902174669489299	8.979743942601476E-5
25	0.0	0.0	0.0	0.010147110655139669	8.979743942601476E-5
26	0.0	0.0	0.0	0.0145920839067274	8.979743942601476E-5
27	0.0	0.0	0.0	0.03322505258762547	8.979743942601476E-5
28	0.0	0.0	0.0	0.07206244513937685	1.3469615913902215E-4
29	0.0	0.0	0.0	0.12580621263584668	1.3469615913902215E-4
30	0.0	0.0	0.0	0.19737477185838045	1.3469615913902215E-4
31	0.0	0.0	0.0	0.3868024703275586	1.3469615913902215E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3070	0.0	18.56026	1
GGACCGT	65	0.0015805626	17.076925	6
GACGGAC	255	0.0	14.509803	7
TACGCTA	115	2.2115737E-5	14.478261	9
GCGCGTT	155	1.2116288E-7	14.322581	25
CTTATAC	1165	0.0	14.133048	37
GTATCAA	4105	0.0	13.880633	2
TATACTG	340	0.0	13.602941	5
GGCGCGT	185	7.392373E-8	13.0	24
TCTATAC	245	1.1459633E-10	12.836736	3
ATACGCT	130	7.0035574E-5	12.8076935	8
TCTTATA	1760	0.0	12.508523	37
AACGCAG	4600	0.0	12.226087	7
TCCGACC	200	2.0421612E-7	12.025001	8
TAGTACG	200	2.0421612E-7	12.025001	4
TCTAGCG	125	7.223368E-4	11.84	28
CGCGTTC	190	1.4352845E-6	11.684211	26
AGTACTA	190	1.4352845E-6	11.684211	5
CTAGTAC	175	7.3406936E-6	11.6285715	3
TACGTTA	175	7.3406936E-6	11.6285715	19
>>END_MODULE