FastQCFastQC Report
Fri 10 Feb 2017
ERR1630329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392540
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43350.18118819330084346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.17842961873155727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34900.1458700794971035No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24470.10227624198550493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.021.1871511
ACCGTCC1351.1532393E-917.8148168
AAGACGG6150.016.8455285
GCGATAG1005.8831356E-616.65000236
AGACGTA700.002593593415.8571425
CGCAAGA6550.015.8167942
GACGGAC6400.015.031257
CAAGACG7000.014.7999994
GCGCAAG6900.014.7463761
GTATTAG6950.014.3741011
TACCGTC4650.014.322587
GTATCAA26150.014.290632
ACGGACC6850.014.0437978
CGAATTA2252.7284841E-1113.977777515
CGGACCA6700.013.805979
TAGACAG3100.013.7258075
GTAAACG4100.013.53658527
CGGGAGT4300.013.3372094
CTAGTAC1254.8486632E-513.323
ATACCGT5150.013.2912636
Produced by FastQC (version 0.11.5)