##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2392540
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89793190500472	33.0	31.0	34.0	30.0	34.0
2	32.11255109632441	33.0	31.0	34.0	30.0	34.0
3	32.244405109214476	34.0	31.0	34.0	30.0	34.0
4	35.86930458842903	37.0	35.0	37.0	35.0	37.0
5	35.79053181973969	37.0	35.0	37.0	35.0	37.0
6	35.83754127412708	37.0	35.0	37.0	35.0	37.0
7	35.811935432636446	37.0	35.0	37.0	35.0	37.0
8	35.81027736213397	37.0	35.0	37.0	35.0	37.0
9	37.55966002658263	39.0	37.0	39.0	35.0	39.0
10	37.453355011828435	39.0	37.0	39.0	34.0	39.0
11	37.51916540580304	39.0	37.0	39.0	35.0	39.0
12	37.44820107500815	39.0	37.0	39.0	35.0	39.0
13	37.453479983615736	39.0	37.0	39.0	35.0	39.0
14	38.71172185209025	40.0	38.0	41.0	35.0	41.0
15	38.71761015489814	40.0	38.0	41.0	35.0	41.0
16	38.67790339973418	40.0	38.0	41.0	34.0	41.0
17	38.64339404983825	40.0	38.0	41.0	34.0	41.0
18	38.592774206491846	40.0	38.0	41.0	34.0	41.0
19	38.618769174183086	40.0	38.0	41.0	34.0	41.0
20	38.598005466993236	40.0	38.0	41.0	34.0	41.0
21	38.53592249241392	40.0	38.0	41.0	34.0	41.0
22	38.49589724727695	40.0	38.0	41.0	34.0	41.0
23	38.43975105954341	40.0	38.0	41.0	34.0	41.0
24	38.40257090790541	40.0	38.0	41.0	34.0	41.0
25	38.38340842786327	40.0	38.0	41.0	34.0	41.0
26	38.222941727202056	40.0	38.0	41.0	34.0	41.0
27	38.10891144975633	40.0	38.0	41.0	33.0	41.0
28	38.026720138430285	40.0	38.0	41.0	33.0	41.0
29	37.97233734859187	40.0	37.0	41.0	33.0	41.0
30	37.90205263025906	40.0	37.0	41.0	33.0	41.0
31	37.85862179942655	40.0	37.0	41.0	33.0	41.0
32	37.76522148010065	40.0	37.0	41.0	33.0	41.0
33	37.67229555200749	40.0	37.0	41.0	32.0	41.0
34	37.619112742106715	40.0	37.0	41.0	32.0	41.0
35	37.48399107224957	40.0	37.0	41.0	32.0	41.0
36	37.39650371571635	40.0	37.0	41.0	31.0	41.0
37	37.340072057311474	40.0	36.0	41.0	31.0	41.0
38	37.2263769884725	40.0	36.0	41.0	31.0	41.0
39	37.1500714721593	39.0	36.0	41.0	31.0	41.0
40	37.04450709288037	39.0	36.0	41.0	31.0	41.0
41	36.925667282469675	39.0	36.0	41.0	30.0	41.0
42	36.886594163524954	39.0	36.0	41.0	30.0	41.0
43	35.66314544375434	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	2.0
12	1.0
13	1.0
14	5.0
15	4.0
16	22.0
17	56.0
18	145.0
19	264.0
20	446.0
21	873.0
22	1593.0
23	2603.0
24	4167.0
25	6368.0
26	9576.0
27	13644.0
28	19609.0
29	26742.0
30	36297.0
31	47327.0
32	61411.0
33	78666.0
34	104498.0
35	138742.0
36	193542.0
37	293782.0
38	548485.0
39	803659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1777692326983	19.284818644620362	12.308132779389268	26.229279343292067
2	18.30991331388399	21.226604361891546	34.52326815852608	25.94021416569838
3	19.39144173138171	23.076897355948073	30.123592500020894	27.408068412649317
4	14.381034381870315	15.711628645707073	35.214249291547894	34.69308768087472
5	14.552274988087973	36.241860115191386	34.53049896762437	14.675365929096273
6	34.20615747281132	35.697334213848045	15.89127036538574	14.205237947954894
7	29.066807660477984	30.783685957183575	21.50772818845244	18.641778193885997
8	26.925276066439853	33.55726550026332	20.410609644979814	19.10684878831702
9	26.588771765571316	14.229772542987787	19.918036898024695	39.2634187934162
10	16.67391140795974	26.404407031857357	33.38978658664014	23.531894973542762
11	35.68224564688573	21.815518235849765	21.645447933994834	20.856788183269664
12	21.168214533508323	25.366263468949317	29.7300358614694	23.73548613607296
13	29.56644403019385	19.507092880369818	25.600491527832347	25.32597156160399
14	22.7414797662735	21.00136256865089	25.03648841816647	31.220669246909143
15	25.003803489178868	27.869251924732712	22.188678141222297	24.938266444866127
16	24.98808797345081	26.699783493692898	23.96540914676453	24.34671938609177
17	23.432251916373396	26.605615789077717	25.705944310230965	24.256187984317922
18	23.764701948556763	25.34289917827915	26.777901309904955	24.11449756325913
19	24.207035201083368	25.494829762511806	26.51454103170689	23.783594004697935
20	24.77538515552509	24.971452932866324	26.125289441346855	24.127872470261728
21	25.034064216272245	25.371989601009805	25.452448025947323	24.14149815677063
22	24.58220134250629	25.203089603517604	25.987026340207482	24.22768271376863
23	23.941459703912997	25.233015957935915	26.14848654567949	24.677037792471598
24	24.05171909351568	25.715390338301553	26.117807852742274	24.115082715440494
25	24.64911767410367	25.207854414137277	25.82435403378836	24.318673877970692
26	24.07127989500698	26.26129552692954	25.81545136131475	23.85197321674873
27	24.77036956539912	25.269253596596087	25.472719369373138	24.487657468631664
28	23.95579593235641	25.531568960184575	26.344721509358255	24.167913598100764
29	23.452857632474274	25.788617954140786	26.53046553035686	24.228058883028076
30	23.618413903215828	26.37611074422998	26.420582310013625	23.584893042540564
31	24.370459846021383	25.98368261345683	25.543648173071297	24.10220936745049
32	23.633084504334306	25.542185292617887	25.99697392729066	24.827756275757146
33	23.459294306469275	25.478947060446224	26.384595450859756	24.67716318222475
34	24.48452272480293	25.18419754737643	26.200774072742778	24.130505655077865
35	24.514323689468096	25.31439390772986	26.81739908214701	23.353883320655036
36	23.53657619099367	25.86640139767778	26.29230859254182	24.30471381878673
37	24.37668753709447	24.931662584533594	26.527080007021826	24.164569871350114
38	23.757387546289717	25.002758574569285	27.08468823927709	24.15516563986391
39	23.97201300709706	25.011243281199057	26.647119797370163	24.369623914333722
40	23.461885694701028	25.003678099425713	27.471724610664815	24.062711595208437
41	22.91100671253145	24.90553971929414	27.984234328370682	24.199219239803725
42	23.821545303317812	24.800170530064282	27.541148737325187	23.83713542929272
43	23.083334029943074	24.252844257567272	27.889523268158527	24.77429844433113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	278.0
1	262.5
2	247.0
3	591.5
4	936.0
5	936.0
6	1251.0
7	1566.0
8	1413.0
9	1260.0
10	1844.5
11	2429.0
12	2429.0
13	4162.5
14	5896.0
15	8564.0
16	11232.0
17	11891.5
18	12551.0
19	12551.0
20	16787.0
21	21023.0
22	23115.0
23	25207.0
24	29915.5
25	34624.0
26	34624.0
27	39248.0
28	43872.0
29	51218.5
30	58565.0
31	67464.5
32	76364.0
33	76364.0
34	84221.0
35	92078.0
36	102908.0
37	113738.0
38	122008.5
39	130279.0
40	130279.0
41	136283.5
42	142288.0
43	141550.0
44	140812.0
45	147561.0
46	154310.0
47	154310.0
48	157148.0
49	159986.0
50	164087.0
51	168188.0
52	175449.5
53	182711.0
54	182711.0
55	175009.5
56	167308.0
57	160607.0
58	153906.0
59	140758.5
60	127611.0
61	127611.0
62	116847.0
63	106083.0
64	92912.0
65	79741.0
66	68762.0
67	57783.0
68	57783.0
69	49718.5
70	41654.0
71	36812.5
72	31971.0
73	25624.0
74	19277.0
75	19277.0
76	14615.5
77	9954.0
78	8388.5
79	6823.0
80	5338.0
81	3853.0
82	3853.0
83	2859.0
84	1865.0
85	1730.5
86	1596.0
87	1496.5
88	1397.0
89	1397.0
90	1219.5
91	1042.0
92	599.5
93	157.0
94	108.0
95	59.0
96	59.0
97	37.0
98	15.0
99	10.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2392540.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.907627746983344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80146561008361	42.750138639089805
2	11.670590585387142	12.349265245582227
3	3.382540257286422	5.36886542315086
4	1.347099034867752	2.8508725710041425
5	0.6704043953024424	1.7734753093301552
6	0.4116639086112679	1.306809650020388
7	0.2622203404882832	0.9711419313568918
8	0.20619164662712686	0.8727288707428451
9	0.14393713640132186	0.6853835188519113
>10	0.8870838256048758	9.046686932527717
>50	0.10033256477539466	3.7420370566688526
>100	0.10128461918609312	11.339158831625092
>500	0.01194321553188534	4.289174999228516
>1k	0.0032428598464058728	2.6542610208207194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4335	0.18118819330084346	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4269	0.17842961873155727	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3490	0.1458700794971035	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2447	0.10227624198550493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.179658438312421E-5	0.0	0.0	0.0	0.0
6	4.179658438312421E-5	0.0	0.0	0.0	0.0
7	4.179658438312421E-5	0.0	0.0	0.0	0.0
8	4.179658438312421E-5	0.0	0.0	0.0	0.0
9	4.179658438312421E-5	0.0	0.0	4.179658438312421E-5	0.0
10	4.179658438312421E-5	0.0	0.0	4.179658438312421E-5	0.0
11	4.179658438312421E-5	0.0	0.0	4.179658438312421E-5	0.0
12	4.179658438312421E-5	0.0	0.0	1.2538975314937262E-4	0.0
13	4.179658438312421E-5	0.0	0.0	1.2538975314937262E-4	0.0
14	4.179658438312421E-5	0.0	0.0	1.2538975314937262E-4	0.0
15	4.179658438312421E-5	0.0	0.0	1.2538975314937262E-4	0.0
16	4.179658438312421E-5	0.0	0.0	1.2538975314937262E-4	0.0
17	4.179658438312421E-5	0.0	0.0	2.0898292191562105E-4	0.0
18	4.179658438312421E-5	0.0	0.0	2.0898292191562105E-4	0.0
19	4.179658438312421E-5	0.0	0.0	3.761692594481179E-4	0.0
20	4.179658438312421E-5	0.0	0.0	4.179658438312421E-4	0.0
21	4.179658438312421E-5	0.0	0.0	7.523385188962358E-4	0.0
22	4.179658438312421E-5	0.0	0.0	0.001003118025194981	0.0
23	4.179658438312421E-5	0.0	0.0	0.0020480326347730865	0.0
24	4.179658438312421E-5	0.0	0.0	0.0028839643224355706	0.0
25	4.179658438312421E-5	0.0	0.0	0.0038452857632474274	0.0
26	4.179658438312421E-5	0.0	0.0	0.006311284241851756	0.0
27	4.179658438312421E-5	0.0	0.0	0.01738737910337967	0.0
28	4.179658438312421E-5	0.0	0.0	0.04317587166776731	0.0
29	4.179658438312421E-5	0.0	0.0	0.07807601962767603	0.0
30	4.179658438312421E-5	0.0	0.0	0.12543154973375575	0.0
31	4.179658438312421E-5	0.0	0.0	0.2636110577043644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	21.187151	1
ACCGTCC	135	1.1532393E-9	17.814816	8
AAGACGG	615	0.0	16.845528	5
GCGATAG	100	5.8831356E-6	16.650002	36
AGACGTA	70	0.0025935934	15.857142	5
CGCAAGA	655	0.0	15.816794	2
GACGGAC	640	0.0	15.03125	7
CAAGACG	700	0.0	14.799999	4
GCGCAAG	690	0.0	14.746376	1
GTATTAG	695	0.0	14.374101	1
TACCGTC	465	0.0	14.32258	7
GTATCAA	2615	0.0	14.29063	2
ACGGACC	685	0.0	14.043797	8
CGAATTA	225	2.7284841E-11	13.9777775	15
CGGACCA	670	0.0	13.80597	9
TAGACAG	310	0.0	13.725807	5
GTAAACG	410	0.0	13.536585	27
CGGGAGT	430	0.0	13.337209	4
CTAGTAC	125	4.8486632E-5	13.32	3
ATACCGT	515	0.0	13.291263	6
>>END_MODULE