FastQCFastQC Report
Fri 10 Feb 2017
ERR1630328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289619
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38990.17029034088204195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31990.13971756873086744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27370.11953953911109229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.022.1308421
CCGTCGA500.007036962518.514
TATACTG2750.017.490915
TATCGTG650.001580581817.0769215
GTATCAA22600.015.8805312
GTTCTAG2900.014.6724151
GTCGTAA908.2784344E-414.3888881
GACGGAC3500.014.2714297
ACCGTCG3400.014.1470598
TCTTATA15750.014.09523837
GTCGACG950.001245733913.63157916
TACCGTC3550.013.5492957
GCTATAC1652.59819E-713.4545453
AGACGGA4400.013.4545456
TCTAGAC3200.013.2968763
CTAGACA3950.013.1139244
ACGGACC3950.013.1139248
GAACGGT850.00940845413.05882420
CTTATAC9950.013.01507637
CGTAAAC1000.001833445912.9500013
Produced by FastQC (version 0.11.5)