FastQCFastQC Report
Fri 10 Feb 2017
ERR1630327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087588
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT198451.8246799339455748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118931.0935207082093588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115931.0659367333953667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61110.5618855669610183No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA37420.34406411251319435No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA25420.2337282132572261No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24110.2216832109217829No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.1657796886320923No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA15220.13994269888965308No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.13415006417871472No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14310.13157555986274214No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.1164963202977598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG1650.025.78787810
AGTCGGT1700.023.94117711
ATAGCAC1059.822543E-1021.1428573
ACGGACC2100.021.1428578
AAGACGG2400.020.81255
AACCGTC450.003825677620.5555557
CGTATAC450.003825677620.5555553
ACCCGTC450.003825677620.55555525
GCGGATA1001.2896635E-820.3523
ACACCGC1101.7553248E-920.1818186
CGGACCA2200.020.1818189
GTTCTAC1850.020.01
CCGCTTA656.901695E-519.92307725
CGCACTA656.901695E-519.92307729
TACACTA656.901695E-519.9230775
GTAGTAC851.2451819E-619.5882363
CGGTGAT2100.019.3809514
CGCAAGA2300.019.3043482
TACACCG1153.0504452E-919.3043485
ACCGGAC1551.8189894E-1219.0967758
Produced by FastQC (version 0.11.5)