FastQCFastQC Report
Fri 10 Feb 2017
ERR1630326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432474
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66280.46269600704794644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43160.3012969170819156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40080.27979565423176966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.13138109312978805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3550.018.7605635
TAGGACG902.1529704E-618.54
CGCCTTA1156.408118E-817.6956525
CGTATAC650.001580190717.0769233
CAAGACG4250.016.5411784
TCTAGCG2250.016.44444528
GACGGAC4000.016.18757
GCGCAAG4350.016.160921
CTAGATA2850.015.5789473
CGGACCA4400.015.5568189
TGAACCG1455.347829E-815.3103455
CTTATAC5800.015.31034537
TTACACT2450.015.1020414
TACCGTC1853.0559022E-1015.07
CGATCGC2850.014.92982419
GTACCGC1001.09366236E-414.86
CTAGCGG2500.014.79999929
GGTATCA24350.014.587271
CGCAAGA4450.014.5505632
GACCGTT908.275771E-414.38888811
Produced by FastQC (version 0.11.5)