FastQCFastQC Report
Fri 10 Feb 2017
ERR1630325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657630
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9580.1456746194668735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.13609476453324817No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8520.12955613338807537No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6590.100208323829509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACT601.3359531E-624.6666684
GTATATG652.679948E-622.769231
TTAGACT502.7003992E-422.1999994
CTAGCAC1202.382876E-1020.0416683
GGTATCA5850.019.2905981
GGACTAG500.0070317618.4999981
TAGCACT1800.018.4999984
TAAGCTT500.0070317618.4999984
TCTAGCA1351.1496013E-917.8148162
ACCTCTA852.7202466E-517.41176425
CCGTCGT752.0655719E-417.2666669
ACCGTCG1005.8701007E-616.658
TCTAGCG904.4413056E-516.44444328
GTTCAAA1606.2755134E-1016.18751
ATACCGT803.379812E-416.18756
TACCGTC803.379812E-416.18757
AAGACGG1151.2401415E-616.0869565
TCTTATA7500.016.03333337
CTTATAC4650.015.91397937
CGTACAC700.002590912415.8571423
Produced by FastQC (version 0.11.5)