##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657630
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835621854234144	33.0	31.0	34.0	30.0	34.0
2	32.05406535589921	33.0	31.0	34.0	30.0	34.0
3	32.17168164469383	34.0	31.0	34.0	30.0	34.0
4	35.81110654927543	37.0	35.0	37.0	35.0	37.0
5	35.736251387558355	37.0	35.0	37.0	35.0	37.0
6	35.79288201572313	37.0	35.0	37.0	35.0	37.0
7	35.76336085640862	37.0	35.0	37.0	33.0	37.0
8	35.757889694813194	37.0	35.0	37.0	33.0	37.0
9	37.514246612837006	39.0	37.0	39.0	35.0	39.0
10	37.40684275352402	39.0	37.0	39.0	34.0	39.0
11	37.47151589799736	39.0	37.0	39.0	35.0	39.0
12	37.39728570776881	39.0	37.0	39.0	34.0	39.0
13	37.40004105652115	39.0	37.0	39.0	34.0	39.0
14	38.63500904764077	40.0	38.0	41.0	34.0	41.0
15	38.64482915925368	40.0	38.0	41.0	34.0	41.0
16	38.58716907683652	40.0	38.0	41.0	34.0	41.0
17	38.55586271915819	40.0	38.0	41.0	34.0	41.0
18	38.50129404072199	40.0	38.0	41.0	34.0	41.0
19	38.536035460669375	40.0	38.0	41.0	34.0	41.0
20	38.510469412891744	40.0	38.0	41.0	34.0	41.0
21	38.44620379240607	40.0	38.0	41.0	34.0	41.0
22	38.41645606191932	40.0	38.0	41.0	34.0	41.0
23	38.351539619542905	40.0	38.0	41.0	34.0	41.0
24	38.309783616927454	40.0	38.0	41.0	34.0	41.0
25	38.28670833143257	40.0	38.0	41.0	34.0	41.0
26	38.11658683454222	40.0	38.0	41.0	33.0	41.0
27	37.992158204461475	40.0	37.0	41.0	33.0	41.0
28	37.910933199519484	40.0	37.0	41.0	33.0	41.0
29	37.86320423338351	40.0	37.0	41.0	33.0	41.0
30	37.79654669038821	40.0	37.0	41.0	33.0	41.0
31	37.73519912412755	40.0	37.0	41.0	32.0	41.0
32	37.64285540501498	40.0	37.0	41.0	32.0	41.0
33	37.549497437768956	40.0	37.0	41.0	32.0	41.0
34	37.49790611742165	40.0	37.0	41.0	32.0	41.0
35	37.35882639174004	40.0	37.0	41.0	31.0	41.0
36	37.276441159922754	40.0	36.0	41.0	31.0	41.0
37	37.22455332025607	40.0	36.0	41.0	31.0	41.0
38	37.10301081155057	39.0	36.0	41.0	31.0	41.0
39	37.02487873120143	39.0	36.0	41.0	31.0	41.0
40	36.926966531332205	39.0	36.0	41.0	30.0	41.0
41	36.81012271337986	39.0	35.0	41.0	30.0	41.0
42	36.779400270668916	39.0	35.0	41.0	30.0	41.0
43	35.47093502425376	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	2.0
16	11.0
17	14.0
18	38.0
19	75.0
20	102.0
21	225.0
22	396.0
23	711.0
24	1196.0
25	1961.0
26	2759.0
27	3996.0
28	5778.0
29	7894.0
30	10469.0
31	13764.0
32	17613.0
33	22905.0
34	29948.0
35	39527.0
36	54819.0
37	82083.0
38	151307.0
39	210030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82944816994358	18.86760032236972	12.786825418548423	26.516126089138268
2	19.163359335796727	20.797560938521663	34.17940179128081	25.859677934400803
3	20.329060413910558	22.311634201602725	29.36195124918267	27.997354135304047
4	15.647096391587976	15.83808524550279	32.82210361449447	35.69271474841476
5	15.359092498821525	35.73726867691559	33.614342411386346	15.28929641287654
6	35.55509937198729	33.754238705655155	15.403646427322354	15.287015495035202
7	29.78148807079969	29.616957863844412	20.898681629487704	19.702872435868194
8	26.61891945318796	33.80213798032328	19.80809877894865	19.77084378754011
9	26.595502030016878	14.568070191445038	19.11561212231802	39.72081565622007
10	17.846205313017958	26.400559585177074	32.086887763636085	23.66634733816888
11	36.28788224381491	21.495065614403234	20.7245715675988	21.492480574183052
12	21.76816751060627	25.142861487462554	28.056962121557717	25.032008880373464
13	30.287699770387604	19.15955780606116	24.85394522755957	25.69879719599167
14	23.500904764077067	20.698112920639264	23.549564344692303	32.25141797059137
15	26.038349831972386	27.24008941197938	21.374024907622825	25.347535848425405
16	25.91761324757082	25.912291105941033	23.17275671730304	24.997338929185105
17	24.690175326551405	26.053403889725224	24.235360308988337	25.021060474735034
18	24.207837233702843	24.653832702279395	25.63477943524474	25.50355062877302
19	25.343278135121572	25.040980490549398	25.398628408071406	24.217112966257623
20	25.43649164423764	24.2344479418518	25.45230600793759	24.876754405972964
21	25.557076167449782	25.077931359579093	24.443380016118486	24.92161245685264
22	25.622614540090932	24.434256344753127	24.463604154311692	25.479524960844245
23	24.720435503246506	24.884813649012365	24.940468044341042	25.454282803400087
24	25.33689156516582	25.3273117102322	24.6215957301218	24.71420099448018
25	25.65545975700622	24.499186472636588	24.74902300685796	25.096330763499232
26	25.425695299788636	25.39345832763104	24.48139531347414	24.699451059106185
27	25.753691285373236	24.41813785867433	24.251934978635404	25.576235877317032
28	24.727886501528214	24.84010765932211	25.235922935389198	25.196082903760477
29	24.682876389459118	25.00053221416298	25.11518635098764	25.201405045390263
30	24.367045299028327	26.029074099417603	25.027751167069628	24.576129434484436
31	25.447744172254914	24.913705275002663	24.659002782719767	24.979547770022656
32	24.36765354378602	24.829159253683684	24.967686997247693	25.835500205282607
33	24.375560725635996	24.699603120295606	25.140428508431796	25.784407645636602
34	25.137235223453917	24.411903349908005	25.09739519182519	25.35346623481289
35	25.3831181667503	24.398065781670546	25.49016924410383	24.728646807475325
36	24.395024557882092	24.70614175144078	25.444398826087617	25.45443486458951
37	25.271201131335246	23.44677098064261	25.66823289691772	25.61379499110442
38	24.38209935678117	23.598832170065233	26.645986344905193	25.37308212824841
39	24.314128005109257	23.245441965847057	26.86571476362088	25.574715265422803
40	23.903562793668172	23.045481501756306	27.593935799765823	25.457019904809698
41	23.113300792238796	23.36100846980825	28.097562459133556	25.428128278819393
42	23.89246232684032	23.003208491096817	27.67528853610693	25.42904064595593
43	22.882015723126987	22.946945851010447	28.077946565698035	26.09309186016453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	28.5
2	32.0
3	82.0
4	132.0
5	132.0
6	192.0
7	252.0
8	245.0
9	238.0
10	374.0
11	510.0
12	510.0
13	875.5
14	1241.0
15	1898.0
16	2555.0
17	2439.0
18	2323.0
19	2323.0
20	2778.5
21	3234.0
22	3458.0
23	3682.0
24	4370.0
25	5058.0
26	5058.0
27	5930.5
28	6803.0
29	9338.0
30	11873.0
31	13464.0
32	15055.0
33	15055.0
34	17235.0
35	19415.0
36	21981.5
37	24548.0
38	28996.5
39	33445.0
40	33445.0
41	36396.0
42	39347.0
43	38968.5
44	38590.0
45	42381.5
46	46173.0
47	46173.0
48	48728.0
49	51283.0
50	53443.5
51	55604.0
52	58174.5
53	60745.0
54	60745.0
55	54543.5
56	48342.0
57	45722.5
58	43103.0
59	39213.5
60	35324.0
61	35324.0
62	32775.5
63	30227.0
64	26974.0
65	23721.0
66	20845.5
67	17970.0
68	17970.0
69	15405.5
70	12841.0
71	11282.0
72	9723.0
73	7822.0
74	5921.0
75	5921.0
76	4642.0
77	3363.0
78	2787.5
79	2212.0
80	1710.0
81	1208.0
82	1208.0
83	914.5
84	621.0
85	488.5
86	356.0
87	324.5
88	293.0
89	293.0
90	241.0
91	189.0
92	109.5
93	30.0
94	22.0
95	14.0
96	14.0
97	10.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	657630.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.67348304790237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.18447644614129	48.27700981814827
2	8.327348301281944	9.438796655733617
3	2.390885110060699	4.064993602635217
4	1.0503195386697917	2.381010662787323
5	0.6010966660649029	1.7033120857189954
6	0.4148666440593867	1.4107162631543881
7	0.28913307314695463	1.1470324823707294
8	0.21225172805699064	0.9623235769540675
9	0.17466322219295516	0.8908895845840006
>10	1.0901788141093163	12.235651663318144
>50	0.16031841201009828	6.507686679991277
>100	0.10204518457106085	10.028470351626767
>500	0.0024168596345761992	0.9521065729770964
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	958	0.1456746194668735	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.13609476453324817	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	852	0.12955613338807537	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	659	0.100208323829509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	3.041223788452473E-4	0.0
15	0.0	0.0	0.0	3.041223788452473E-4	0.0
16	0.0	0.0	0.0	3.041223788452473E-4	0.0
17	0.0	0.0	0.0	6.082447576904946E-4	0.0
18	0.0	0.0	0.0	6.082447576904946E-4	0.0
19	0.0	0.0	0.0	0.0010644283259583656	0.0
20	0.0	0.0	0.0	0.0015206118942262367	0.0
21	0.0	0.0	0.0	0.002280917841339355	0.0
22	0.0	0.0	0.0	0.0028891625990298494	0.0
23	0.0	0.0	0.0	0.004713896872101333	0.0
24	0.0	0.0	0.0	0.007755120660553807	0.0
25	0.0	0.0	0.0	0.009731916123047914	0.0
26	0.0	0.0	0.0	0.015206118942262366	0.0
27	0.0	0.0	0.0	0.031020482642215227	0.0
28	0.0	0.0	0.0	0.0790718184997643	0.0
29	0.0	0.0	0.0	0.14324164043611148	0.0
30	0.0	0.0	0.0	0.23554278241564405	0.0
31	0.0	0.0	0.0	0.49571947751775314	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACACT	60	1.3359531E-6	24.666668	4
GTATATG	65	2.679948E-6	22.76923	1
TTAGACT	50	2.7003992E-4	22.199999	4
CTAGCAC	120	2.382876E-10	20.041668	3
GGTATCA	585	0.0	19.290598	1
GGACTAG	50	0.00703176	18.499998	1
TAGCACT	180	0.0	18.499998	4
TAAGCTT	50	0.00703176	18.499998	4
TCTAGCA	135	1.1496013E-9	17.814816	2
ACCTCTA	85	2.7202466E-5	17.411764	25
CCGTCGT	75	2.0655719E-4	17.266666	9
ACCGTCG	100	5.8701007E-6	16.65	8
TCTAGCG	90	4.4413056E-5	16.444443	28
GTTCAAA	160	6.2755134E-10	16.1875	1
ATACCGT	80	3.379812E-4	16.1875	6
TACCGTC	80	3.379812E-4	16.1875	7
AAGACGG	115	1.2401415E-6	16.086956	5
TCTTATA	750	0.0	16.033333	37
CTTATAC	465	0.0	15.913979	37
CGTACAC	70	0.0025909124	15.857142	3
>>END_MODULE