##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630324.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6861
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75528348637225	31.0	31.0	34.0	30.0	34.0
2	31.9903804110188	33.0	31.0	34.0	30.0	34.0
3	32.03760384783559	33.0	31.0	34.0	30.0	34.0
4	35.70427051450226	37.0	35.0	37.0	33.0	37.0
5	35.639265413205074	37.0	35.0	37.0	33.0	37.0
6	35.743769129864454	37.0	35.0	37.0	35.0	37.0
7	35.70368750910946	37.0	35.0	37.0	33.0	37.0
8	35.72642471942866	37.0	35.0	37.0	33.0	37.0
9	37.45110042267891	39.0	37.0	39.0	35.0	39.0
10	37.39527765631832	39.0	37.0	39.0	34.0	39.0
11	37.42253315843172	39.0	37.0	39.0	34.0	39.0
12	37.34324442501093	39.0	37.0	39.0	34.0	39.0
13	37.27663605888355	39.0	37.0	39.0	34.0	39.0
14	38.4901617839965	40.0	38.0	41.0	34.0	41.0
15	38.46859058446291	40.0	38.0	41.0	34.0	41.0
16	38.42034688820872	40.0	38.0	41.0	33.0	41.0
17	38.45984550357091	40.0	38.0	41.0	34.0	41.0
18	38.325608511878734	40.0	38.0	41.0	33.0	41.0
19	38.30301705290774	40.0	38.0	41.0	34.0	41.0
20	38.25535636204635	40.0	38.0	41.0	33.0	41.0
21	38.21993878443376	40.0	38.0	41.0	33.0	41.0
22	38.172715347616965	40.0	38.0	41.0	33.0	41.0
23	38.11470631103338	40.0	37.0	41.0	33.0	41.0
24	38.10537822474858	40.0	37.0	41.0	33.0	41.0
25	38.03774959918379	40.0	37.0	41.0	33.0	41.0
26	37.85497740854103	40.0	37.0	41.0	33.0	41.0
27	37.70427051450226	40.0	37.0	41.0	32.0	41.0
28	37.61055239760968	40.0	37.0	41.0	32.0	41.0
29	37.5254336102609	40.0	37.0	41.0	32.0	41.0
30	37.42675994752951	40.0	37.0	41.0	31.0	41.0
31	37.32487975513774	40.0	36.0	41.0	31.0	41.0
32	37.181023174464364	39.0	36.0	41.0	31.0	41.0
33	37.10712724092698	39.0	36.0	41.0	30.0	41.0
34	37.05407375018219	39.0	36.0	41.0	30.0	41.0
35	36.92697857455182	39.0	36.0	41.0	30.0	41.0
36	36.87407083515522	39.0	35.0	41.0	30.0	41.0
37	36.68357382305786	39.0	35.0	41.0	30.0	41.0
38	36.61915172715348	39.0	35.0	41.0	30.0	41.0
39	36.4888500218627	39.0	35.0	40.0	30.0	41.0
40	36.46669581693631	39.0	35.0	40.0	30.0	41.0
41	36.272700772482146	39.0	35.0	40.0	29.0	41.0
42	36.24427925958315	38.0	35.0	40.0	30.0	41.0
43	34.940241947238015	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	1.0
22	6.0
23	11.0
24	15.0
25	22.0
26	33.0
27	54.0
28	76.0
29	87.0
30	113.0
31	159.0
32	223.0
33	278.0
34	334.0
35	451.0
36	625.0
37	895.0
38	1590.0
39	1884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84521206821163	16.74682990817665	11.747558664917651	29.660399358694068
2	22.620609240635474	17.985716367876403	31.91954525579362	27.474129135694504
3	21.133945488995774	20.21571199533596	28.100859932954382	30.54948258271389
4	17.679638536656466	14.035854831657193	29.93732692027401	38.34717971141233
5	17.082058009036583	35.33012680367293	31.64261769421367	15.94519749307681
6	40.781227226351845	31.20536364961376	13.423699169217315	14.589709954817081
7	33.10013117621338	27.270077248214548	18.816499052616237	20.813292522955837
8	29.79157557207404	32.5025506485935	17.315260166156538	20.390613613175923
9	27.29922751785454	12.694942428217463	17.09663314385658	42.909196910071415
10	20.30316280425594	24.573677306515084	29.23772044891415	25.885439440314823
11	40.839527765631836	19.137152018656174	18.233493659816354	21.78982655589564
12	24.20929893601516	22.955837341495407	25.929164844774817	26.905698877714617
13	33.391633872613326	15.95977262789681	21.78982655589564	28.858766943594226
14	24.107272992275178	18.10231744643638	21.512898994315695	36.277510566972744
15	28.071709663314387	25.433610260894913	19.25375309721615	27.240926978574553
16	27.503279405334503	24.107272992275178	20.274012534615945	28.115435067774374
17	26.41014429383472	23.407666520915317	21.090220084535783	29.09196910071418
18	26.65792158577467	21.571199533595685	23.80119516105524	27.969683719574405
19	28.421512898994315	21.70237574697566	21.55662439877569	28.319486955254337
20	27.85308264101443	21.877277364815626	21.80440169071564	28.465238303454303
21	31.219938784433754	22.766360588835447	20.69669144439586	25.31700918233494
22	28.69844046057426	22.183355196035563	21.512898994315695	27.605305349074477
23	27.867657775834427	22.722635184375456	21.133945488995774	28.275761550794343
24	29.06281883107419	22.576883836175483	21.76067628625565	26.599621046494683
25	27.882232910654427	21.76067628625565	21.716950881795658	28.64013992129427
26	28.975368022154207	22.635184375455474	21.235971432735752	27.153476169654567
27	29.485497740854104	21.527474129135697	21.221396297915756	27.765631832094446
28	28.479813438274306	20.84244279259583	22.28538113977554	28.392362629354324
29	26.905698877714617	22.270806004955546	22.70806004955546	28.115435067774374
30	27.25550211339455	22.70806004955546	22.037603847835594	27.9988339892144
31	27.503279405334503	22.75178545401545	21.921002769275617	27.82393237137444
32	26.963999416994604	22.460282757615506	21.877277364815626	28.69844046057426
33	27.240926978574553	22.518583296895496	21.818976825535636	28.421512898994315
34	28.20288587669436	21.585774668415684	23.043288150415393	27.168051304474567
35	28.59641451683428	22.21250546567556	21.979303308555604	27.21177670893456
36	27.182626439294566	23.05786328523539	21.133945488995774	28.62556478647427
37	26.70164699023466	22.41655735315552	22.984987611135402	27.89680804547442
38	28.304911820434338	21.20682116309576	23.087013554875384	27.40125346159452
39	25.739688092114854	21.133945488995774	24.282174610115142	28.844191808774234
40	25.695962687654863	21.046494680075792	26.439294563474714	26.818248068794638
41	25.900014575134822	20.274012534615945	26.176942136714764	27.649030753534472
42	25.404459991254917	21.075644949715784	26.20609240635476	27.313802652674536
43	24.19472380119516	19.516105523976098	27.357528057134527	28.931642617694216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.0
11	0.0
12	0.0
13	2.5
14	5.0
15	7.0
16	9.0
17	7.5
18	6.0
19	6.0
20	6.5
21	7.0
22	8.0
23	9.0
24	10.0
25	11.0
26	11.0
27	18.0
28	25.0
29	34.5
30	44.0
31	55.0
32	66.0
33	66.0
34	83.0
35	100.0
36	124.5
37	149.0
38	179.0
39	209.0
40	209.0
41	228.0
42	247.0
43	275.5
44	304.0
45	331.5
46	359.0
47	359.0
48	395.5
49	432.0
50	479.5
51	527.0
52	562.5
53	598.0
54	598.0
55	646.5
56	695.0
57	659.0
58	623.0
59	579.5
60	536.0
61	536.0
62	533.5
63	531.0
64	453.5
65	376.0
66	312.5
67	249.0
68	249.0
69	232.5
70	216.0
71	186.5
72	157.0
73	150.5
74	144.0
75	144.0
76	132.5
77	121.0
78	95.5
79	70.0
80	44.0
81	18.0
82	18.0
83	12.0
84	6.0
85	4.0
86	2.0
87	2.0
88	2.0
89	2.0
90	4.0
91	6.0
92	3.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6861.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.83617548462323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48122392211405	76.69435942282466
2	4.58970792767733	7.695671184958461
3	1.8254520166898471	4.591167468299082
4	0.7301808066759388	2.4486226497595105
5	0.39986091794158557	1.6761405042996649
6	0.33031988873435325	1.6615653694796677
7	0.22600834492350488	1.3263372686197348
8	0.12169680111265646	0.8162075499198368
9	0.08692628650904034	0.6558810668998688
>10	0.2086230876216968	2.4340475149395133
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	38	0.5538551231598892	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	16	0.23320215711995335	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	15	0.21862702229995626	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	13	0.1894767526599621	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	12	0.17490161783996502	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	12	0.17490161783996502	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	11	0.16032648301996794	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	10	0.14575134819997085	No Hit
CCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG	10	0.14575134819997085	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	10	0.14575134819997085	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	10	0.14575134819997085	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	10	0.14575134819997085	No Hit
GATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCCTGGAGCT	9	0.13117621337997376	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	9	0.13117621337997376	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	9	0.13117621337997376	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	9	0.13117621337997376	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	9	0.13117621337997376	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	8	0.11660107855997667	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	8	0.11660107855997667	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	8	0.11660107855997667	No Hit
CATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAACTAGACG	8	0.11660107855997667	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	8	0.11660107855997667	No Hit
CTGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATG	8	0.11660107855997667	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	8	0.11660107855997667	No Hit
CCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGA	7	0.1020259437399796	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	7	0.1020259437399796	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	7	0.1020259437399796	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	7	0.1020259437399796	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	7	0.1020259437399796	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	7	0.1020259437399796	No Hit
GCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCGGGG	7	0.1020259437399796	No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG	7	0.1020259437399796	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	7	0.1020259437399796	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCCT	7	0.1020259437399796	No Hit
CTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCCCACCCGCC	7	0.1020259437399796	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	7	0.1020259437399796	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	7	0.1020259437399796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.014575134819997084	0.0
29	0.0	0.0	0.0	0.058300539279988337	0.0
30	0.0	0.0	0.0	0.11660107855997667	0.0
31	0.0	0.0	0.0	0.2915026963999417	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE