FastQCFastQC Report
Fri 10 Feb 2017
ERR1630323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709198
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT217090.8013072503375537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135690.5008493288419673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134500.4964568850264912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70450.2600400561346937No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC48750.17994255126424868No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA35600.13140420153861032No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT29580.10918360341326105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15950.019.6018837
GACAGGC14600.019.006857
TAGACCG500.007037287618.4999985
CGGACCA4950.018.3131319
TGCATCA14600.018.24657414
ACGGACC5000.018.138
CGAATTA3000.017.88333315
AAGACGG5600.017.8392855
GACGGAC4900.017.3673467
CAGGACA17800.017.356744
GCATCAG15600.017.31410215
TATACTG3100.017.3064525
CCAGGAC18650.017.061663
CGCAAGA5250.016.9142862
TCTAGCG2300.016.89130428
ACAGGCT16800.016.6279778
GCTGCAT15900.016.52201312
ATCAAGC15850.016.22397430
CAAGACG6200.016.1129044
GGACAGG18150.016.1046856
Produced by FastQC (version 0.11.5)