##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2709198
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.670768249496714	31.0	31.0	34.0	30.0	34.0
2	31.863115578853964	33.0	31.0	34.0	30.0	34.0
3	31.99803816480006	33.0	31.0	34.0	30.0	34.0
4	35.68806303562899	37.0	35.0	37.0	33.0	37.0
5	35.60964241077987	37.0	35.0	37.0	33.0	37.0
6	35.66762968228974	37.0	35.0	37.0	33.0	37.0
7	35.64020791392877	37.0	35.0	37.0	33.0	37.0
8	35.624371492965814	37.0	35.0	37.0	33.0	37.0
9	37.29544573707791	39.0	37.0	39.0	34.0	39.0
10	37.194356411011675	39.0	37.0	39.0	34.0	39.0
11	37.2812204940355	39.0	37.0	39.0	34.0	39.0
12	37.164162235465994	39.0	37.0	39.0	33.0	39.0
13	37.204048209100996	39.0	37.0	39.0	34.0	39.0
14	38.359010674007585	40.0	38.0	41.0	34.0	41.0
15	38.33891801189873	40.0	38.0	41.0	34.0	41.0
16	38.325672394561046	40.0	38.0	41.0	34.0	41.0
17	38.31275307304966	40.0	38.0	41.0	33.0	41.0
18	38.28278516372742	40.0	38.0	41.0	33.0	41.0
19	38.305068141937205	40.0	38.0	41.0	34.0	41.0
20	38.25777333365815	40.0	38.0	41.0	34.0	41.0
21	38.2230099092056	40.0	38.0	41.0	33.0	41.0
22	38.184375966614475	40.0	38.0	41.0	33.0	41.0
23	38.125036265344946	40.0	38.0	41.0	33.0	41.0
24	38.10045740473749	40.0	37.0	41.0	33.0	41.0
25	38.07049872323839	40.0	37.0	41.0	33.0	41.0
26	37.87818793606078	40.0	37.0	41.0	33.0	41.0
27	37.76963182462116	40.0	37.0	41.0	33.0	41.0
28	37.66377134487771	40.0	37.0	41.0	32.0	41.0
29	37.563434270953984	40.0	37.0	41.0	32.0	41.0
30	37.4789908304967	39.0	37.0	41.0	32.0	41.0
31	37.34110648243502	39.0	36.0	41.0	32.0	41.0
32	37.2169778657743	39.0	36.0	41.0	31.0	41.0
33	37.12878866734731	39.0	36.0	41.0	31.0	41.0
34	37.0046515610893	39.0	36.0	41.0	31.0	41.0
35	36.88702929796936	39.0	35.0	41.0	31.0	41.0
36	36.763084130432695	39.0	35.0	40.0	30.0	41.0
37	36.67112112145365	39.0	35.0	40.0	30.0	41.0
38	36.54194562376024	39.0	35.0	40.0	30.0	41.0
39	36.417127504154365	39.0	35.0	40.0	30.0	41.0
40	36.27426566829003	39.0	35.0	40.0	30.0	41.0
41	36.10388498736526	39.0	35.0	40.0	29.0	41.0
42	36.001194449427466	39.0	35.0	40.0	29.0	41.0
43	34.802912522451294	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	9.0
11	12.0
12	12.0
13	6.0
14	7.0
15	12.0
16	32.0
17	65.0
18	147.0
19	353.0
20	683.0
21	1242.0
22	2233.0
23	3598.0
24	5947.0
25	9137.0
26	13775.0
27	19883.0
28	27567.0
29	37852.0
30	50270.0
31	64613.0
32	83141.0
33	106298.0
34	138906.0
35	183896.0
36	253358.0
37	384305.0
38	647048.0
39	674787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.42203781340456	19.254923412759055	12.858491701234092	25.4645470726023
2	17.75673095875606	21.83756964238125	35.05310427661618	25.35259512224651
3	19.214210257057623	23.751161782933547	30.7265471183723	26.308080841636528
4	14.027066312613549	15.716791463746835	35.258331063288836	34.99781116035078
5	14.187335144939572	37.5524417189146	33.71713695344526	14.543086182700563
6	33.61507722949744	36.100351469327826	15.964134035238473	14.320437265936267
7	28.72661946450573	31.70185420187081	21.241452267423792	18.330074066199664
8	28.156524550808026	33.71558667915745	19.75182323329635	18.376065536738178
9	27.22554792968251	13.94874054978632	19.900575742341463	38.925135778189706
10	16.94556839330311	27.584953185407635	32.20325720010129	23.26622122118797
11	35.14719116136953	22.587385639587804	21.43169306931424	20.83373012972843
12	21.753633363083836	26.262089371098014	29.280510320766517	22.703766945051633
13	30.04265468969046	19.51319172685053	25.290399594271072	25.153753989187944
14	22.440700162926444	20.88326508435338	26.253304483467062	30.422730269253112
15	24.991455035770734	27.82081634491093	22.81763828262091	24.370090336697427
16	23.7466216939478	26.894970393452233	24.37241574812915	24.98599216447081
17	22.667556967043385	27.046528160732436	26.592297794402626	23.693617077821553
18	22.720524671877065	25.279400029086098	27.812880417009016	24.187194882027818
19	23.42626858575859	25.62175226764526	27.896041559162526	23.055937587433625
20	23.750128266741672	25.69553794148674	27.4436198461685	23.110713945603088
21	25.030027336503274	25.48370403344458	26.62249123172245	22.86377739832969
22	24.4733312220074	25.911136801370738	26.705430906120558	22.910101070501305
23	23.631458461138685	25.805312125581075	26.774344289343194	23.78888512393705
24	23.636293840464965	25.959933530144347	27.007549835781656	23.39622279360903
25	23.710079514306447	25.55560723136515	27.152721949447773	23.581591304880632
26	23.715653119484067	26.214547626271685	27.11643814885438	22.95336110538986
27	23.777258066778433	26.16361004252919	26.59672714951067	23.462404741181707
28	23.13972622156077	25.974402756830617	27.657446964009274	23.228424057599334
29	22.84056019530503	26.177857801460064	27.886629179557936	23.09495282367697
30	22.80730312070214	26.696646018489606	27.45657570985952	23.039475150948732
31	23.095137380139803	26.69557559100516	26.892681893313075	23.316605135541955
32	22.471631826097614	26.45561527802693	27.38116593914509	23.69158695673037
33	22.79405196667058	26.447273325906785	27.495037276714363	23.263637430708275
34	23.42479213405591	26.305091026938598	27.212481332113786	23.057635506891707
35	23.333805797878192	26.217426707091914	27.786230463775624	22.662537031254267
36	22.886551665843545	26.575281688529223	26.83100312343358	23.707163522193653
37	22.70247504981179	25.754706743471683	27.72049145171375	23.822326755002774
38	22.786558974279473	25.69413531236919	28.099201313451434	23.420104399899895
39	22.171173904602025	25.832700304665806	28.361050022921912	23.63507576781025
40	22.391497409934598	25.288923142568393	29.11998310939252	23.199596338104485
41	21.48056362067298	25.397996012103953	29.41878002272259	23.702660344500476
42	21.67205940651071	25.764525147294513	28.98374352852763	23.57967191766715
43	21.367467420247614	24.607134657562867	29.653794222496842	24.371603699692677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	395.0
1	420.0
2	445.0
3	1415.0
4	2385.0
5	2385.0
6	3222.5
7	4060.0
8	3944.5
9	3829.0
10	5143.0
11	6457.0
12	6457.0
13	12286.5
14	18116.0
15	30959.5
16	43803.0
17	37992.0
18	32181.0
19	32181.0
20	36615.5
21	41050.0
22	37217.0
23	33384.0
24	39407.5
25	45431.0
26	45431.0
27	50285.0
28	55139.0
29	61765.0
30	68391.0
31	77879.0
32	87367.0
33	87367.0
34	97205.0
35	107043.0
36	119012.0
37	130981.0
38	136553.5
39	142126.0
40	142126.0
41	149602.5
42	157079.0
43	159325.0
44	161571.0
45	165402.5
46	169234.0
47	169234.0
48	169648.0
49	170062.0
50	170531.0
51	171000.0
52	173954.5
53	176909.0
54	176909.0
55	174291.0
56	171673.0
57	164002.5
58	156332.0
59	143555.0
60	130778.0
61	130778.0
62	121894.0
63	113010.0
64	99331.5
65	85653.0
66	72884.5
67	60116.0
68	60116.0
69	52519.5
70	44923.0
71	39609.0
72	34295.0
73	33843.0
74	33391.0
75	33391.0
76	29679.5
77	25968.0
78	20017.0
79	14066.0
80	9533.5
81	5001.0
82	5001.0
83	3595.0
84	2189.0
85	1757.0
86	1325.0
87	1185.5
88	1046.0
89	1046.0
90	924.5
91	803.0
92	463.5
93	124.0
94	85.0
95	46.0
96	46.0
97	30.5
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2709198.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.99882440900513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42457704944457	39.485624098861116
2	13.119695379048801	13.381780818714917
3	4.265425307023009	6.525950287877796
4	1.8282227748297433	3.7294884909634485
5	0.8966685564146559	2.2864521130833575
6	0.5312337452672253	1.625537789701283
7	0.3408459234753773	1.2167918981292176
8	0.24119350916115295	0.9840468337841128
9	0.16996429862564186	0.7801181479267938
>10	0.9802735096669318	9.424410431979908
>50	0.10002781479430863	3.558979626625953
>100	0.08930547820684358	9.294182353091767
>500	0.00946110160625714	3.2727839366311713
>1k	0.002816663836971991	2.379597089782224
>5k	7.222214966594849E-5	0.25948459117598627
>10k+	2.1666644899784546E-4	1.7947714916711792
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21709	0.8013072503375537	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13569	0.5008493288419673	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13450	0.4964568850264912	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7045	0.2600400561346937	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4875	0.17994255126424868	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3560	0.13140420153861032	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2958	0.10918360341326105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.1073387770107611E-4	0.0	0.0	0.0	0.0
6	1.1073387770107611E-4	0.0	0.0	0.0	0.0
7	1.1073387770107611E-4	0.0	0.0	0.0	0.0
8	1.1073387770107611E-4	0.0	0.0	0.0	0.0
9	1.1073387770107611E-4	0.0	0.0	0.0	0.0
10	1.1073387770107611E-4	0.0	0.0	0.0	0.0
11	1.4764517026810147E-4	0.0	0.0	0.0	0.0
12	1.4764517026810147E-4	0.0	0.0	7.382258513405074E-5	0.0
13	1.4764517026810147E-4	0.0	0.0	1.8455646283512686E-4	0.0
14	1.4764517026810147E-4	0.0	0.0	2.583790479691776E-4	0.0
15	2.9529034053620294E-4	0.0	0.0	3.3220163310322833E-4	0.0
16	3.3220163310322833E-4	0.0	0.0	9.96604899309685E-4	0.0
17	3.691129256702537E-4	0.0	0.0	0.0014764517026810149	0.0
18	4.0602421823727905E-4	0.0	0.0	0.0016610081655161417	0.0
19	4.4293551080430444E-4	0.0	0.0	0.0019193872134853193	0.0
20	4.4293551080430444E-4	0.0	0.0	0.0023254114317225985	0.0
21	4.4293551080430444E-4	0.0	0.0	0.0035434840864344355	0.0
22	4.4293551080430444E-4	0.0	0.0	0.004909201911414375	0.0
23	4.4293551080430444E-4	0.0	0.0	0.007382258513405074	0.0
24	5.167580959383552E-4	0.0	0.0	0.010778097429571408	0.0
25	5.167580959383552E-4	0.0	0.0	0.012476016887654576	0.0
26	5.536693885053806E-4	0.0	0.0	0.01531818641531553	0.0
27	5.536693885053806E-4	0.0	0.0	0.028126404936073334	0.0
28	5.536693885053806E-4	0.0	0.0	0.06850735900439908	0.0
29	5.905806810724059E-4	0.0	0.0	0.1319578709271157	0.0
30	6.644032662064567E-4	0.0	0.0	0.23040028820337236	0.0
31	6.644032662064567E-4	0.0	0.0	0.5210767171686972	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1595	0.0	19.60188	37
GACAGGC	1460	0.0	19.00685	7
TAGACCG	50	0.0070372876	18.499998	5
CGGACCA	495	0.0	18.313131	9
TGCATCA	1460	0.0	18.246574	14
ACGGACC	500	0.0	18.13	8
CGAATTA	300	0.0	17.883333	15
AAGACGG	560	0.0	17.839285	5
GACGGAC	490	0.0	17.367346	7
CAGGACA	1780	0.0	17.35674	4
GCATCAG	1560	0.0	17.314102	15
TATACTG	310	0.0	17.306452	5
CCAGGAC	1865	0.0	17.06166	3
CGCAAGA	525	0.0	16.914286	2
TCTAGCG	230	0.0	16.891304	28
ACAGGCT	1680	0.0	16.627977	8
GCTGCAT	1590	0.0	16.522013	12
ATCAAGC	1585	0.0	16.223974	30
CAAGACG	620	0.0	16.112904	4
GGACAGG	1815	0.0	16.104685	6
>>END_MODULE