FastQCFastQC Report
Fri 10 Feb 2017
ERR1630322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120459
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG42570.3799335807914435No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30390.2712281306143286No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA29840.26631942802012387No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23640.21098496241272552No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT23570.21036021844619035No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT23080.20598701068044434No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA18510.16520015457950715No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA18090.16145169078029628No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC16380.1461900881692235No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC16350.14592234075499416No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG16050.1432448666127007No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC16000.14279862092231843No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC15630.13949640281348982No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT15280.1363726829808141No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC15150.13521244418582026No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC14770.1318209769389152No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA14510.12950049934892754No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA14490.12932200107277464No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC14460.12905425365854528No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA14190.12664452693048117No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA14050.1253950389974109No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC13850.1236100562358819No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT13400.11959384502244169No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG13020.11620237777553663No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG12760.11388190018554896No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12600.11245391397632577No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC12400.11066893121479679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.10825920448673267No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC11960.10674196913943304No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG11750.1048677372398276No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA11670.104153744135216No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG11440.1021010139594577No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT11400.10174401740715187No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11400.10174401740715187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG509.090822E-625.9000025
GGTATCA6200.020.2903211
GTATTAA1052.2591848E-819.380951
CGTTATT1153.0504452E-919.3043482
GCTTAGG9350.018.9946521
CTTAGGA9800.018.52
GCGTACA609.236617E-418.52
TAGGACA10450.018.234454
TTAGGAC10500.018.147623
CTAGATA1451.546141E-1017.8620683
GCGTTAT1451.546141E-1017.8620681
CAAGACG2400.017.7291684
GTCGCCA7000.017.70714212
CTCTATG1054.7970025E-717.6190471
TCGCCAT7150.017.59440613
CGGCCTT6100.017.59016424
GTAGAAC8550.017.5263163
TAGAACA8900.017.4606744
GCCATTG7150.017.33566515
AGACGGA2350.017.3191476
Produced by FastQC (version 0.11.5)