FastQCFastQC Report
Fri 10 Feb 2017
ERR1630321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424816
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC127620.3726331575185353No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT68610.2003319302409239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65490.19122195177784732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63380.18506103685570263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49670.14502968918622197No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG47270.13802201344539386No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT46750.13650368370154775No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG46240.1350145526066218No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG46170.13481016206418095No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT42040.12275112006017257No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT40240.1174953632545515No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG40180.11732017136103078No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG35580.10388879285777687No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT34920.10196168202904915No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG34760.10149450364632727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.018.0779481
GACAGGC34250.017.5547457
TGCATCA33100.017.54984914
CAGGACA37550.016.8002664
CCAGGAC39550.016.7926673
GCTGCAT35050.016.6262512
ACAGGCT36100.016.5526318
GCATCAG35300.016.508515
CTGCATC35650.016.4502113
CGCGAAC904.4500943E-516.44444528
ATCAAGC36050.015.80582530
TCCAGGA41150.015.7800732
GGACAGG41300.015.5883786
CGCACTA957.0662005E-515.57894829
AAGACGG5850.015.4957275
GGCCATC38150.015.08125826
TATACTG2700.015.0740755
GACGGAC5800.014.9913797
ATCAGAA39150.014.83780417
CGGATCG1252.9627026E-614.80000137
Produced by FastQC (version 0.11.5)