FastQCFastQC Report
Fri 10 Feb 2017
ERR1630320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717792
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68070.2504606680717288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58360.21473313631065216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45650.16796723222380522No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG44200.1626320189330162No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT40690.14971712331186493No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG32960.1212749172857967No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA32170.11836814590667719No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC31930.11748507612061557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30810.1133640837856613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC1501.2732926E-1118.54
GGTATCA29550.018.3434851
CTTATAC11350.015.97356837
TGATCGC700.002593714515.85714210
GTATTAG3950.015.4556961
ATAACGC1802.0190782E-1015.4166663
AAGACGG2650.015.3584915
TATATCG1001.09423614E-414.85
CTAATCG750.00410632314.826
GCTAGAT6500.014.5153853
ACGGACC2450.014.3469388
CGTCGTA1304.44905E-614.23076910
GTGCTAG7650.014.0261441
TTAGTAC1203.303618E-513.8749993
AACGCCG1753.5715857E-813.7428575
TCTATAC3100.013.7258063
ATACCGT2304.0017767E-1113.6739136
ATATCGC950.001245838913.6315796
TATTAGC3150.013.50793652
TACGTTA1102.4582943E-413.45454519
Produced by FastQC (version 0.11.5)