##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630320.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2717792
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.898427841424216	33.0	31.0	34.0	30.0	34.0
2	32.09705010537966	33.0	31.0	34.0	30.0	34.0
3	32.241511859627224	34.0	31.0	34.0	30.0	34.0
4	35.877123782835476	37.0	35.0	37.0	35.0	37.0
5	35.789602000447424	37.0	35.0	37.0	35.0	37.0
6	35.854591521352624	37.0	35.0	37.0	35.0	37.0
7	35.83294269760158	37.0	35.0	37.0	35.0	37.0
8	35.816232073683345	37.0	35.0	37.0	35.0	37.0
9	37.524060340158485	39.0	37.0	39.0	35.0	39.0
10	37.45048958860722	39.0	37.0	39.0	34.0	39.0
11	37.52509058824222	39.0	37.0	39.0	35.0	39.0
12	37.41991808055951	39.0	37.0	39.0	34.0	39.0
13	37.44964147366686	39.0	37.0	39.0	34.0	39.0
14	38.70405277519398	40.0	38.0	41.0	35.0	41.0
15	38.712019904392974	40.0	38.0	41.0	35.0	41.0
16	38.684911501689605	40.0	38.0	41.0	35.0	41.0
17	38.66180634868305	40.0	38.0	41.0	34.0	41.0
18	38.63524912870448	40.0	38.0	41.0	34.0	41.0
19	38.66876972189189	40.0	38.0	41.0	34.0	41.0
20	38.62619545572288	40.0	38.0	41.0	34.0	41.0
21	38.587857716852504	40.0	38.0	41.0	34.0	41.0
22	38.57178032756002	40.0	38.0	41.0	34.0	41.0
23	38.528054759157435	40.0	38.0	41.0	34.0	41.0
24	38.524579143657796	40.0	38.0	41.0	34.0	41.0
25	38.49735520599074	40.0	38.0	41.0	34.0	41.0
26	38.358350455075296	40.0	38.0	41.0	34.0	41.0
27	38.2743852362506	40.0	38.0	41.0	34.0	41.0
28	38.21229365602666	40.0	38.0	41.0	34.0	41.0
29	38.15682620303541	40.0	38.0	41.0	33.0	41.0
30	38.11012100999635	40.0	38.0	41.0	33.0	41.0
31	38.01335937408014	40.0	37.0	41.0	33.0	41.0
32	37.920247024054824	40.0	37.0	41.0	33.0	41.0
33	37.879083461869044	40.0	37.0	41.0	33.0	41.0
34	37.79528970576115	40.0	37.0	41.0	33.0	41.0
35	37.73384387031826	40.0	37.0	41.0	33.0	41.0
36	37.64644902921195	40.0	37.0	41.0	33.0	41.0
37	37.59639626579223	40.0	37.0	41.0	32.0	41.0
38	37.50348003084857	40.0	37.0	41.0	32.0	41.0
39	37.424019571770025	40.0	37.0	41.0	32.0	41.0
40	37.34771387950219	40.0	37.0	41.0	31.0	41.0
41	37.23058644664492	40.0	36.0	41.0	31.0	41.0
42	37.20192273728085	40.0	36.0	41.0	31.0	41.0
43	36.09804245505175	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	2.0
13	1.0
14	1.0
15	7.0
16	7.0
17	42.0
18	67.0
19	162.0
20	305.0
21	614.0
22	1089.0
23	2006.0
24	3325.0
25	5435.0
26	8594.0
27	13023.0
28	19205.0
29	27027.0
30	37536.0
31	50025.0
32	66084.0
33	87279.0
34	116456.0
35	157478.0
36	222041.0
37	337374.0
38	634241.0
39	928354.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.649029800661715	19.80306071987849	13.079735314549456	25.46817416491034
2	17.54122464117931	21.266307355382605	36.534657545536966	24.65781045790112
3	19.475662596696143	23.349763337297336	30.790840505822377	26.383733560184147
4	14.772212148685405	15.800362941682073	34.80571728815156	34.621707621480965
5	13.895287056551789	37.304216069515256	34.86981343678986	13.930683437143093
6	34.0532314467038	35.3144390740719	15.90088571899542	14.73144376022889
7	28.238253700062405	31.35217853316221	21.630610436707446	18.77895733006794
8	25.764039337815404	35.69125231069927	20.211002166464542	18.33370618502078
9	25.837002978888744	15.27791677950336	20.2822732571146	38.60280698449329
10	16.55700657003921	27.831563269006605	33.888428547880046	21.723001613074143
11	34.47920223475528	22.635139112926964	21.92198667153336	20.9636719807844
12	20.244963558653495	26.31599474867834	30.013812683236978	23.425229009431188
13	29.101932745405097	20.626670473678633	25.976748772533	24.294648008383277
14	22.09801191555498	21.79092439745205	25.530209817381166	30.5808538696118
15	24.56600799472513	28.684093558300265	23.115860227714265	23.634038219260344
16	24.202992723504963	27.765737775370596	24.4117651387597	23.619504362364744
17	22.810686027481132	27.6781298936784	26.126281922972765	23.384902155867703
18	22.11979430361117	26.545924044224133	27.58901343443501	23.745268217729688
19	23.44671704089202	26.61811499923467	27.73078292967232	22.20438503020099
20	23.74412758592269	25.936274740671838	27.750394437837773	22.5692032355677
21	23.228194063416186	26.770849277648917	26.555564222721973	23.445392436212924
22	23.432440746017356	26.27853787191956	26.743547703429844	23.545473678633243
23	22.85785667188659	26.870231423155268	26.91291312948158	23.358998775476564
24	23.942487136616784	26.684749973507905	26.68662649680329	22.686136393072022
25	23.78504315267688	26.10107763949559	26.921854211065455	23.19202499676208
26	23.546503926716984	26.601189495001826	26.670326500335566	23.181980077945628
27	23.65324498710718	26.08378418951855	26.479068302504388	23.78390252086988
28	22.968166805995455	26.513103283842177	27.154211948522917	23.364517961639446
29	23.020967020287056	26.876044965913504	26.990917627250354	23.11207038654908
30	22.78434111219696	27.590374833688525	26.704214303375686	22.921069750738834
31	23.438401467073273	26.710947710494402	27.00640814308085	22.844242679351474
32	22.541680893902107	26.835681317775606	27.08441264084963	23.538225147472655
33	22.890346281098775	26.48554414760217	27.12915484334342	23.494954727955637
34	23.104417115069882	26.168926834724658	27.294325687911364	23.4323303622941
35	23.362641438344067	26.35370918745805	27.114804959319915	23.16884441487796
36	22.941233177520576	26.681291283512497	27.086730699038043	23.290744839928884
37	23.739638647843545	25.57016872520046	26.940509060296	23.749683566659996
38	23.006653930837974	26.161862276436164	27.748775476563324	23.08270831616253
39	22.92228397169467	25.802563257232343	27.97833682636493	23.296815944708058
40	22.719509072070267	25.212746229292016	28.662642321413855	23.405102377223862
41	22.103457512569026	25.84679033568426	28.69958407413076	23.350168077615947
42	22.65360998928542	25.17470063934252	28.357247353734206	23.814442017637845
43	22.033805383193418	25.410774628816334	28.467741460715168	24.087678527275084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	506.0
1	468.5
2	431.0
3	893.5
4	1356.0
5	1356.0
6	1775.0
7	2194.0
8	2086.0
9	1978.0
10	3004.0
11	4030.0
12	4030.0
13	6822.5
14	9615.0
15	14068.0
16	18521.0
17	19023.5
18	19526.0
19	19526.0
20	23295.5
21	27065.0
22	30085.5
23	33106.0
24	37836.5
25	42567.0
26	42567.0
27	48512.0
28	54457.0
29	69528.0
30	84599.0
31	90250.0
32	95901.0
33	95901.0
34	104476.0
35	113051.0
36	121258.5
37	129466.0
38	145443.0
39	161420.0
40	161420.0
41	172212.0
42	183004.0
43	175068.0
44	167132.0
45	176247.5
46	185363.0
47	185363.0
48	191628.5
49	197894.0
50	203172.0
51	208450.0
52	213612.0
53	218774.0
54	218774.0
55	193930.0
56	169086.0
57	158652.0
58	148218.0
59	132934.0
60	117650.0
61	117650.0
62	107665.5
63	97681.0
64	85871.0
65	74061.0
66	63378.5
67	52696.0
68	52696.0
69	44862.5
70	37029.0
71	31450.0
72	25871.0
73	20918.5
74	15966.0
75	15966.0
76	12393.5
77	8821.0
78	7044.5
79	5268.0
80	3876.5
81	2485.0
82	2485.0
83	1836.5
84	1188.0
85	932.0
86	676.0
87	518.5
88	361.0
89	361.0
90	306.5
91	252.0
92	150.0
93	48.0
94	35.0
95	22.0
96	22.0
97	14.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2717792.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.99398801770854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01237747685099	42.931689612943224
2	11.442133295792702	12.127285495485248
3	3.3630675936307295	5.346670912788324
4	1.3783524707929824	2.921775772855291
5	0.7186300974210137	1.9041537385946958
6	0.42811196319872014	1.3612416148794393
7	0.2893233470062221	1.0732678589143745
8	0.1976468998622524	0.8379277954429954
9	0.14155017716534546	0.6751177553344354
>10	0.8358430489573961	8.35273136601019
>50	0.08811171796452606	3.274691917372089
>100	0.08742751568849505	9.793611646401821
>500	0.012356742745174122	4.4633496103398995
>1k	0.004928813117451384	4.471369476110167
>5k	1.3883980612539113E-4	0.4651154265277379
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6807	0.2504606680717288	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5836	0.21473313631065216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4565	0.16796723222380522	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4420	0.1626320189330162	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	4069	0.14971712331186493	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3296	0.1212749172857967	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3217	0.11836814590667719	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3193	0.11748507612061557	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3081	0.1133640837856613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.6794574419234435E-5	0.0	0.0	0.0
3	0.0	3.6794574419234435E-5	0.0	0.0	0.0
4	0.0	3.6794574419234435E-5	0.0	0.0	0.0
5	0.0	3.6794574419234435E-5	0.0	0.0	0.0
6	0.0	3.6794574419234435E-5	0.0	0.0	0.0
7	0.0	3.6794574419234435E-5	0.0	0.0	0.0
8	0.0	3.6794574419234435E-5	0.0	0.0	0.0
9	0.0	3.6794574419234435E-5	0.0	3.6794574419234435E-5	0.0
10	0.0	3.6794574419234435E-5	0.0	7.358914883846887E-5	0.0
11	0.0	3.6794574419234435E-5	0.0	7.358914883846887E-5	0.0
12	0.0	7.358914883846887E-5	0.0	7.358914883846887E-5	0.0
13	0.0	7.358914883846887E-5	0.0	1.1038372325770331E-4	0.0
14	0.0	7.358914883846887E-5	0.0	1.4717829767693774E-4	0.0
15	0.0	7.358914883846887E-5	0.0	2.943565953538755E-4	0.0
16	0.0	7.358914883846887E-5	0.0	5.88713190707751E-4	0.0
17	0.0	7.358914883846887E-5	0.0	7.358914883846887E-4	0.0
18	0.0	7.358914883846887E-5	0.0	8.830697860616265E-4	0.0
19	0.0	7.358914883846887E-5	0.0	0.001103837232577033	0.0
20	0.0	1.1038372325770331E-4	0.0	0.0013246046790924397	0.0
21	0.0	1.1038372325770331E-4	0.0	0.001950112444219425	0.0
22	0.0	1.1038372325770331E-4	0.0	0.0028699768047002862	0.0
23	0.0	1.1038372325770331E-4	0.0	0.0040841977605350225	0.0
24	0.0	1.1038372325770331E-4	0.0	0.0057767481838198066	0.0
25	0.0	1.1038372325770331E-4	0.0	0.006954174565235309	0.0
26	0.0	1.1038372325770331E-4	0.0	0.009088259881550906	0.0
27	0.0	1.1038372325770331E-4	0.0	0.019427535293355783	0.0
28	0.0	1.1038372325770331E-4	0.0	0.04713384983103931	0.0
29	0.0	1.1038372325770331E-4	0.0	0.08676160648055481	0.0
30	0.0	1.1038372325770331E-4	0.0	0.1443451154466567	0.0
31	0.0	1.1038372325770331E-4	0.0	0.3152559136240007	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	150	1.2732926E-11	18.5	4
GGTATCA	2955	0.0	18.343485	1
CTTATAC	1135	0.0	15.973568	37
TGATCGC	70	0.0025937145	15.857142	10
GTATTAG	395	0.0	15.455696	1
ATAACGC	180	2.0190782E-10	15.416666	3
AAGACGG	265	0.0	15.358491	5
TATATCG	100	1.09423614E-4	14.8	5
CTAATCG	75	0.004106323	14.8	26
GCTAGAT	650	0.0	14.515385	3
ACGGACC	245	0.0	14.346938	8
CGTCGTA	130	4.44905E-6	14.230769	10
GTGCTAG	765	0.0	14.026144	1
TTAGTAC	120	3.303618E-5	13.874999	3
AACGCCG	175	3.5715857E-8	13.742857	5
TCTATAC	310	0.0	13.725806	3
ATACCGT	230	4.0017767E-11	13.673913	6
ATATCGC	95	0.0012458389	13.631579	6
TATTAGC	315	0.0	13.5079365	2
TACGTTA	110	2.4582943E-4	13.454545	19
>>END_MODULE