FastQCFastQC Report
Fri 10 Feb 2017
ERR1630319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408881
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG30180.21421255592204025No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT26730.1897250371039144No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG24520.17403882939723087No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC23450.1664441496478411No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG21890.1553715324431233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.14422793692299066No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA19730.14004021631351407No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC19080.13542662581154832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17380.12336031219102252No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT17260.12250857240604424No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA17020.12080509283608765No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG16420.11654639391119619No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT15700.11143595520132644No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA15530.11022932383927386No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC15400.10930660573888072No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG14830.10526084176023384No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC14800.10504790681398926No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC14720.10448008029067041No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC14610.10369931882110697No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG14190.10071822957368294No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA14110.10015040305036409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA200.001842077237.01
GGTATCA10700.020.4018691
TATACTG1700.019.5882345
AGGCCCG2750.018.16363710
ACCGACC953.607558E-617.5263148
CTAGTAC953.607558E-617.5263143
CAAGACG2800.017.1785724
CTTATAC6250.017.16837
GACGGAC2700.017.129637
AAGACGG2600.017.0769235
TCTAGCG2400.016.95833428
CTAGCGG2450.016.61224429
CGGTCCA2900.016.58620610
CGAGCCG2700.016.44444515
TCTTATA11150.016.42600837
TTACACA2150.016.3488374
AGTACCG1151.2426044E-616.0869565
GGACGAT9750.015.74871712
GGTCCAA3600.015.41666611
CGCAATA2650.015.35849136
Produced by FastQC (version 0.11.5)