##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630319.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1408881
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896409278001478	33.0	31.0	34.0	30.0	34.0
2	32.09595700417565	33.0	31.0	34.0	30.0	34.0
3	32.2487491846366	34.0	31.0	34.0	30.0	34.0
4	35.88628989957278	37.0	35.0	37.0	35.0	37.0
5	35.80750254989598	37.0	35.0	37.0	35.0	37.0
6	35.86514616919385	37.0	35.0	37.0	35.0	37.0
7	35.83267146054209	37.0	35.0	37.0	35.0	37.0
8	35.814134763688344	37.0	35.0	37.0	35.0	37.0
9	37.54543144523917	39.0	37.0	39.0	35.0	39.0
10	37.45755816140611	39.0	37.0	39.0	35.0	39.0
11	37.54014569009022	39.0	37.0	39.0	35.0	39.0
12	37.396507582968326	39.0	37.0	39.0	34.0	39.0
13	37.44692489997381	39.0	37.0	39.0	35.0	39.0
14	38.67990128335892	40.0	38.0	41.0	35.0	41.0
15	38.6755425050093	40.0	38.0	41.0	35.0	41.0
16	38.66439181165762	40.0	38.0	41.0	35.0	41.0
17	38.611708866824095	40.0	38.0	41.0	34.0	41.0
18	38.57671158884249	40.0	38.0	41.0	34.0	41.0
19	38.579801984695656	40.0	38.0	41.0	34.0	41.0
20	38.544935306814416	40.0	38.0	41.0	34.0	41.0
21	38.49718819403484	40.0	38.0	41.0	34.0	41.0
22	38.47108662832418	40.0	38.0	41.0	34.0	41.0
23	38.42442335442099	40.0	38.0	41.0	34.0	41.0
24	38.40707980304937	40.0	38.0	41.0	34.0	41.0
25	38.37633483594427	40.0	38.0	41.0	34.0	41.0
26	38.231774010721985	40.0	38.0	41.0	34.0	41.0
27	38.138254401897676	40.0	38.0	41.0	33.0	41.0
28	38.05247284901989	40.0	38.0	41.0	33.0	41.0
29	37.98248539088823	40.0	37.0	41.0	33.0	41.0
30	37.933602625062015	40.0	37.0	41.0	33.0	41.0
31	37.817613410926825	40.0	37.0	41.0	33.0	41.0
32	37.72641337345028	40.0	37.0	41.0	33.0	41.0
33	37.670610221871115	40.0	37.0	41.0	32.0	41.0
34	37.563335725302565	40.0	37.0	41.0	32.0	41.0
35	37.491782485532845	40.0	37.0	41.0	32.0	41.0
36	37.38631651644106	40.0	37.0	41.0	32.0	41.0
37	37.32273130235981	39.0	36.0	41.0	31.0	41.0
38	37.24181460322057	39.0	36.0	41.0	31.0	41.0
39	37.12521568535597	39.0	36.0	41.0	31.0	41.0
40	37.027989588900695	39.0	36.0	41.0	31.0	41.0
41	36.91730316471015	39.0	35.0	41.0	31.0	41.0
42	36.85862468157353	39.0	35.0	41.0	31.0	41.0
43	35.627495153955515	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	0.0
14	2.0
15	1.0
16	10.0
17	21.0
18	49.0
19	99.0
20	208.0
21	413.0
22	761.0
23	1243.0
24	1984.0
25	3124.0
26	4971.0
27	7239.0
28	10532.0
29	14924.0
30	20270.0
31	27175.0
32	35521.0
33	47222.0
34	63479.0
35	86008.0
36	123810.0
37	187706.0
38	331991.0
39	440114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67473193264726	18.57012764030461	11.783748946859244	25.97139148018889
2	18.978252953940043	20.083669238211037	34.483111064738615	26.454966743110315
3	20.23982153212372	21.091632295417426	28.93757528137579	29.730970891083064
4	13.92048015410812	15.217395933368397	34.550895355959796	36.311228556563684
5	15.491017339292673	35.455443007606746	33.815560008261876	15.237979644838706
6	36.5487219999418	34.424908846098425	15.25089769824421	13.775471455715566
7	31.671447056209857	29.339525481570128	19.821262406122305	19.16776505609771
8	28.234322132245378	33.0909423861916	19.32675648262699	19.347978998936036
9	26.946278642411958	13.267053782398937	18.76368550644093	41.02298206874818
10	17.048068644548405	26.069128620515148	32.615742564489125	24.267060170447326
11	38.003280617738476	20.901268453474778	20.005380156308448	21.0900707724783
12	22.373216758548097	24.459766296798666	28.444985772396674	24.722031172256564
13	31.638938987749853	18.278051872372473	24.23114514284741	25.851863997030268
14	23.669635689600472	19.906933232828038	23.885054876884563	32.53837620068693
15	25.671862989138187	27.02187054832878	21.73135985225154	25.574906610281495
16	26.975521708362876	25.09346069682251	22.624125103539615	25.30689249127499
17	24.69285908462106	25.323146525504992	23.99762648513253	25.98636790474142
18	24.69754365343844	23.70292451953004	25.92624927158504	25.673282555446487
19	26.441693798127737	24.583126608989687	24.687393754334114	24.287785838548466
20	26.795165808893724	23.950922753589552	24.520949604686272	24.73296183283045
21	26.358010364253616	24.44784193980897	23.866103666668796	25.32804402926862
22	26.83881747287386	23.49240283600957	24.41639854608019	25.252381145036377
23	24.612653588202267	24.11488266219787	24.78073023910465	26.49173351049521
24	25.277436490377823	24.78562774286828	24.547850386228504	25.389085380525394
25	25.65262786566076	24.353653715253454	24.491493603789106	25.50222481529668
26	26.12832453557114	24.721605302364075	23.65267187221632	25.497398289848466
27	26.234792008693425	23.968099505919945	23.627758483505705	26.169350001880925
28	25.456585758484923	24.412139847155295	24.72785139412058	25.403423000239194
29	25.31512597586311	24.93588883660153	25.145203888759944	24.603781298775413
30	25.601665435192896	24.56793724949091	24.135111482091105	25.69528583322509
31	25.21440774628943	24.42825192475447	24.331792394105676	26.025547934850422
32	24.387865263283416	24.715572145553814	24.31589325145275	26.580669339710024
33	24.906858705596854	23.773618921683237	25.050660772627353	26.268861600092556
34	26.07147090492384	23.721875729745808	25.1488237828461	25.057829582484253
35	26.30768673862448	23.776883924192322	24.591005202000737	25.32442413518246
36	24.54983777906012	24.47985316006107	24.147674643919537	26.822634416959275
37	25.054493601659754	23.88391922383793	25.841430184664283	25.22015698983803
38	24.560839417949424	23.370746003388504	25.26295691403319	26.80545766462888
39	24.332502177259826	23.915930444090026	26.34608600726392	25.40548137138623
40	25.278501165109045	22.981358965022597	26.174815332167867	25.565324537700484
41	23.527891993717	23.646922628667717	27.089725817865386	25.735459559749902
42	23.878666828497224	24.00174322742659	26.80545766462888	25.314132279447303
43	23.83068548727678	22.42105614313771	26.773588401007608	26.974669968577903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	24.5
2	41.0
3	117.0
4	193.0
5	193.0
6	292.5
7	392.0
8	381.0
9	370.0
10	515.5
11	661.0
12	661.0
13	1211.5
14	1762.0
15	3018.0
16	4274.0
17	4186.5
18	4099.0
19	4099.0
20	5074.5
21	6050.0
22	6514.0
23	6978.0
24	8952.0
25	10926.0
26	10926.0
27	13301.5
28	15677.0
29	19040.0
30	22403.0
31	26460.5
32	30518.0
33	30518.0
34	39048.5
35	47579.0
36	52768.5
37	57958.0
38	61750.0
39	65542.0
40	65542.0
41	69419.0
42	73296.0
43	78060.5
44	82825.0
45	85570.0
46	88315.0
47	88315.0
48	91010.5
49	93706.0
50	96788.0
51	99870.0
52	108515.0
53	117160.0
54	117160.0
55	115874.5
56	114589.0
57	106663.0
58	98737.0
59	97386.0
60	96035.0
61	96035.0
62	88102.5
63	80170.0
64	65962.5
65	51755.0
66	46278.5
67	40802.0
68	40802.0
69	36022.0
70	31242.0
71	30826.5
72	30411.0
73	26446.5
74	22482.0
75	22482.0
76	13717.0
77	4952.0
78	3810.5
79	2669.0
80	2256.5
81	1844.0
82	1844.0
83	1431.0
84	1018.0
85	851.0
86	684.0
87	578.0
88	472.0
89	472.0
90	409.0
91	346.0
92	197.5
93	49.0
94	32.0
95	15.0
96	15.0
97	9.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1408881.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.68755157973629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.99540796054528	48.74869124383875
2	9.104857239070299	10.322641287318659
3	2.116200883399704	3.5988674019241267
4	0.8112567428707146	1.8395263382356994
5	0.41463707356011215	1.1752380247154883
6	0.26129021809661285	0.8887141629379371
7	0.18282107551448198	0.7254575403663043
8	0.1282700918872009	0.581705395999457
9	0.09461715868734208	0.4827253557075118
>10	0.6923682580769556	7.692016765660894
>50	0.08959325845295035	3.588029011471395
>100	0.08973008821636912	10.704001211775106
>500	0.014181089624524612	5.508006734949985
>1k	0.004768861997627746	4.144379525098759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	3018	0.21421255592204025	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	2673	0.1897250371039144	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	2452	0.17403882939723087	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	2345	0.1664441496478411	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	2189	0.1553715324431233	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2032	0.14422793692299066	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1973	0.14004021631351407	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1908	0.13542662581154832	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1738	0.12336031219102252	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1726	0.12250857240604424	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	1702	0.12080509283608765	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	1642	0.11654639391119619	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	1570	0.11143595520132644	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	1553	0.11022932383927386	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	1540	0.10930660573888072	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1483	0.10526084176023384	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1480	0.10504790681398926	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	1472	0.10448008029067041	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1461	0.10369931882110697	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1419	0.10071822957368294	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	1411	0.10015040305036409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.09783154148576E-5
7	7.09783154148576E-5	0.0	0.0	0.0	7.09783154148576E-5
8	7.09783154148576E-5	0.0	0.0	0.0	7.09783154148576E-5
9	2.1293494624457281E-4	0.0	0.0	7.09783154148576E-5	7.09783154148576E-5
10	2.1293494624457281E-4	0.0	0.0	7.09783154148576E-5	7.09783154148576E-5
11	2.1293494624457281E-4	0.0	0.0	7.09783154148576E-5	7.09783154148576E-5
12	2.1293494624457281E-4	0.0	0.0	7.09783154148576E-5	7.09783154148576E-5
13	2.1293494624457281E-4	0.0	0.0	7.09783154148576E-5	7.09783154148576E-5
14	2.1293494624457281E-4	0.0	0.0	7.09783154148576E-5	7.09783154148576E-5
15	2.839132616594304E-4	0.0	0.0	2.839132616594304E-4	1.419566308297152E-4
16	2.839132616594304E-4	0.0	0.0	4.968482079040033E-4	1.419566308297152E-4
17	2.839132616594304E-4	0.0	0.0	7.807614695634336E-4	1.419566308297152E-4
18	2.839132616594304E-4	0.0	0.0	8.517397849782913E-4	1.419566308297152E-4
19	3.54891577074288E-4	0.0	0.0	0.0010646747312228642	1.419566308297152E-4
20	3.54891577074288E-4	0.0	0.0	0.0010646747312228642	1.419566308297152E-4
21	3.54891577074288E-4	0.0	0.0	0.0011356530466377216	2.1293494624457281E-4
22	3.54891577074288E-4	0.0	0.0	0.0015615229391268673	2.1293494624457281E-4
23	3.54891577074288E-4	0.0	0.0	0.002839132616594304	2.1293494624457281E-4
24	3.54891577074288E-4	0.0	0.0	0.0038328290324023107	2.1293494624457281E-4
25	3.54891577074288E-4	0.0	0.0	0.004613590501965744	2.1293494624457281E-4
26	3.54891577074288E-4	0.0	0.0	0.0065300050181669	2.1293494624457281E-4
27	3.54891577074288E-4	0.0	0.0	0.013130988351748658	2.1293494624457281E-4
28	3.54891577074288E-4	0.0	0.0	0.038186333693193394	2.1293494624457281E-4
29	3.54891577074288E-4	0.0	0.0	0.07977962652629995	2.1293494624457281E-4
30	3.54891577074288E-4	0.0	0.0	0.1367752138044306	2.1293494624457281E-4
31	3.54891577074288E-4	0.0	0.0	0.37767561632245733	2.1293494624457281E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGA	20	0.0018420772	37.0	1
GGTATCA	1070	0.0	20.401869	1
TATACTG	170	0.0	19.588234	5
AGGCCCG	275	0.0	18.163637	10
ACCGACC	95	3.607558E-6	17.526314	8
CTAGTAC	95	3.607558E-6	17.526314	3
CAAGACG	280	0.0	17.178572	4
CTTATAC	625	0.0	17.168	37
GACGGAC	270	0.0	17.12963	7
AAGACGG	260	0.0	17.076923	5
TCTAGCG	240	0.0	16.958334	28
CTAGCGG	245	0.0	16.612244	29
CGGTCCA	290	0.0	16.586206	10
CGAGCCG	270	0.0	16.444445	15
TCTTATA	1115	0.0	16.426008	37
TTACACA	215	0.0	16.348837	4
AGTACCG	115	1.2426044E-6	16.086956	5
GGACGAT	975	0.0	15.748717	12
GGTCCAA	360	0.0	15.416666	11
CGCAATA	265	0.0	15.358491	36
>>END_MODULE