FastQCFastQC Report
Fri 10 Feb 2017
ERR1630318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049537
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC20580.19608646479352324No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA15450.1472077687589861No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT15440.14711248864975698No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA14760.14063344122217702No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG13020.12405470221631062No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG12220.11643229347798123No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG11730.1117635681257545No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG10950.10433171960588336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA303.5992666E-430.8333342
AGTATCG358.8688784E-426.4285729
ATCGGAT358.8688784E-426.42857212
TTCGTTC1005.347829E-1022.229
CGTTTAG502.701885E-422.226
TTTCGTT1151.3460522E-1020.91304428
GGTATCA7050.020.4680841
CTTATAC3450.020.37681237
TATCGCG759.264515E-619.7333347
CAATACG759.264515E-619.7333344
TGACCGG851.2450964E-619.58823610
TCTAGCG1103.8506187E-818.528
ACCTAGG5100.018.1372571
CGGACCA1451.546141E-1017.8620689
TTAGAGT953.6057827E-617.5263164
CGATTTT953.6057827E-617.52631621
CCGATTT852.7227507E-517.41176420
GCGAAAG1603.45608E-1117.3437518
ACGCTAT752.067051E-417.26666610
TCGCGCG752.067051E-417.2666669
Produced by FastQC (version 0.11.5)