##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1049537
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.887499916629903	33.0	31.0	34.0	30.0	34.0
2	32.07902341699244	33.0	31.0	34.0	30.0	34.0
3	32.230594061953035	34.0	31.0	34.0	30.0	34.0
4	35.861073978335206	37.0	35.0	37.0	35.0	37.0
5	35.77773627799687	37.0	35.0	37.0	35.0	37.0
6	35.85095427793399	37.0	35.0	37.0	35.0	37.0
7	35.827300038016766	37.0	35.0	37.0	35.0	37.0
8	35.80736267516057	37.0	35.0	37.0	35.0	37.0
9	37.52356801141837	39.0	37.0	39.0	35.0	39.0
10	37.44213114925915	39.0	37.0	39.0	34.0	39.0
11	37.52405298717434	39.0	37.0	39.0	35.0	39.0
12	37.402548933482095	39.0	37.0	39.0	34.0	39.0
13	37.43822657038294	39.0	37.0	39.0	34.0	39.0
14	38.65863899986375	40.0	38.0	41.0	35.0	41.0
15	38.66503324799412	40.0	38.0	41.0	35.0	41.0
16	38.64426313698326	40.0	38.0	41.0	34.0	41.0
17	38.61444046279455	40.0	38.0	41.0	34.0	41.0
18	38.58294276428558	40.0	38.0	41.0	34.0	41.0
19	38.58133157763852	40.0	38.0	41.0	34.0	41.0
20	38.54035827226672	40.0	38.0	41.0	34.0	41.0
21	38.49070209054088	40.0	38.0	41.0	34.0	41.0
22	38.45646127768721	40.0	38.0	41.0	34.0	41.0
23	38.42115237480908	40.0	38.0	41.0	34.0	41.0
24	38.39856241371195	40.0	38.0	41.0	34.0	41.0
25	38.36331258450155	40.0	38.0	41.0	34.0	41.0
26	38.21954442768573	40.0	38.0	41.0	34.0	41.0
27	38.12031019392361	40.0	38.0	41.0	33.0	41.0
28	38.03677716936134	40.0	38.0	41.0	33.0	41.0
29	37.98165286216684	40.0	37.0	41.0	33.0	41.0
30	37.92960705530153	40.0	37.0	41.0	33.0	41.0
31	37.8390128218443	40.0	37.0	41.0	33.0	41.0
32	37.76439134589824	40.0	37.0	41.0	33.0	41.0
33	37.7037836684176	40.0	37.0	41.0	33.0	41.0
34	37.62425050284077	40.0	37.0	41.0	32.0	41.0
35	37.53175352560224	40.0	37.0	41.0	32.0	41.0
36	37.441321268330704	40.0	37.0	41.0	32.0	41.0
37	37.38622268676569	40.0	37.0	41.0	31.0	41.0
38	37.28735909262846	39.0	36.0	41.0	31.0	41.0
39	37.191432984258775	39.0	36.0	41.0	31.0	41.0
40	37.09665119000093	39.0	36.0	41.0	31.0	41.0
41	36.993315147536485	39.0	36.0	41.0	31.0	41.0
42	36.94131793352688	39.0	36.0	41.0	31.0	41.0
43	35.688828502472994	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	1.0
12	0.0
13	3.0
14	0.0
15	1.0
16	10.0
17	12.0
18	39.0
19	78.0
20	131.0
21	296.0
22	544.0
23	868.0
24	1500.0
25	2382.0
26	3595.0
27	5268.0
28	7735.0
29	10867.0
30	14828.0
31	19973.0
32	26738.0
33	35159.0
34	46974.0
35	64345.0
36	91032.0
37	137504.0
38	256613.0
39	323034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.852193872155055	18.042432043844094	11.521937768749458	26.58343631525139
2	20.16260503441041	20.207672526075786	31.642047874443683	27.98767456507012
3	21.379998990030842	21.376664186207822	28.204436813566364	29.038900010194972
4	15.568960408256213	14.358807740937193	33.180249957838555	36.89198189296804
5	15.81097188569817	35.208572923108	33.65703162442105	15.323423566772776
6	37.54484120140595	32.88974090479898	14.501442064453181	15.063975829341892
7	31.226150197658587	28.74315055114779	19.948415348863353	20.082283902330268
8	29.469756664129036	30.520505708707745	19.408748810189635	20.60098881697358
9	27.743376365006668	13.218971794229265	18.265292219331002	40.77235962143307
10	18.30092698018269	25.348606099642033	31.902924813513007	24.447542106662272
11	37.74588223187939	20.626238045919294	19.57425035992061	22.053629362280702
12	23.80573529089494	24.32444020553825	27.09823474541631	24.7715897581505
13	32.68155386613335	18.249666281417426	23.091420312004246	25.97735954044498
14	24.293760010366476	19.458389747098007	23.02300919357774	33.22484104895778
15	27.070889354067557	26.481677158594696	20.472074829186585	25.975358658151166
16	26.816110341988896	24.843049840072336	21.952727726607066	26.3881120913317
17	25.626633458372595	24.50594881361972	23.196895392920876	26.670522335086805
18	25.399390397861154	23.44262279462277	24.886973970426958	26.271012837089113
19	26.605541300592545	23.477018914054483	24.4973736037891	25.42006618156387
20	27.48926431369261	23.076461334855274	23.443956716151977	25.990317635300137
21	27.1534972087692	23.145539414046386	23.61231666915983	26.088646708024587
22	26.93959336354983	23.4976946977572	23.933505917371185	25.62920602132178
23	26.30579007695775	23.362301662542624	23.425186534633845	26.906721725865783
24	25.98098018459568	23.9564684236954	24.279563274091338	25.782988117617577
25	26.55447116204574	23.970855720188997	23.288840698326975	26.185832419438288
26	26.520837283487865	24.167418585528665	23.394696899680525	25.91704723130295
27	26.677191942732843	22.90333737638597	23.84337093404044	26.576099746840747
28	26.111418654130347	23.631944371661028	23.510557512503134	26.746079461705495
29	25.79213500810357	23.95894570653536	24.337398300393414	25.911520984967655
30	26.14838733651124	24.340351983779517	23.398317543831233	26.11294313587801
31	26.01775830675812	24.706132323110094	23.68358619086321	25.59252317926857
32	25.34107897101293	23.949608255830903	23.632611332425633	27.07670144073053
33	24.900408465828267	24.313292432758445	24.198670461355817	26.587628640057474
34	26.751986828477705	22.73250014053816	24.197050699498924	26.318462331485215
35	25.95887519925453	23.230719831697215	24.44230170065467	26.36810326839359
36	25.23779533260857	24.532436683985416	24.299095696483306	25.93067228692271
37	26.437181347584698	23.245488248627726	24.503090410342846	25.814239993444726
38	26.18649938020289	23.572108463065142	24.072614876845694	26.168777279886275
39	25.748877838513557	23.142490450551055	24.696223191750267	26.412408519185128
40	24.868108508799594	22.704297228206343	25.46722983563228	26.960364427361778
41	23.976477246633515	23.041112414331273	26.197647152982697	26.78476318605252
42	24.919178647346403	22.647605563215016	26.558568206742596	25.874647582695985
43	24.927849137286252	22.24609518292352	26.18497489845522	26.64108078133501
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	20.0
2	26.0
3	73.0
4	120.0
5	120.0
6	166.0
7	212.0
8	199.0
9	186.0
10	267.0
11	348.0
12	348.0
13	669.5
14	991.0
15	1357.0
16	1723.0
17	1967.0
18	2211.0
19	2211.0
20	2926.5
21	3642.0
22	4048.0
23	4454.0
24	5730.5
25	7007.0
26	7007.0
27	8658.5
28	10310.0
29	12635.5
30	14961.0
31	18092.0
32	21223.0
33	21223.0
34	24374.5
35	27526.0
36	31250.5
37	34975.0
38	38551.0
39	42127.0
40	42127.0
41	45110.5
42	48094.0
43	50237.5
44	52381.0
45	56793.0
46	61205.0
47	61205.0
48	66634.0
49	72063.0
50	74629.0
51	77195.0
52	80572.0
53	83949.0
54	83949.0
55	82934.0
56	81919.0
57	81983.5
58	82048.0
59	81268.0
60	80488.0
61	80488.0
62	74387.0
63	68286.0
64	62546.5
65	56807.0
66	49552.0
67	42297.0
68	42297.0
69	36805.5
70	31314.0
71	26063.0
72	20812.0
73	14707.5
74	8603.0
75	8603.0
76	6492.5
77	4382.0
78	3494.0
79	2606.0
80	2026.0
81	1446.0
82	1446.0
83	1076.0
84	706.0
85	551.5
86	397.0
87	327.0
88	257.0
89	257.0
90	216.0
91	175.0
92	104.5
93	34.0
94	24.5
95	15.0
96	15.0
97	8.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1049537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.08126110862666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40570401261175	53.388506291776764
2	7.833396673279733	9.569474951360938
3	1.8923391670748528	3.4675938831054047
4	0.7871557222748164	1.9232185682167071
5	0.43902383264393013	1.3408064677316953
6	0.2811333998307748	1.0303189560851698
7	0.1858822230318885	0.7947744420323931
8	0.13386873904434093	0.6541497123079995
9	0.10845306344132173	0.5962004897480843
>10	0.7474974092607547	9.042673001606003
>50	0.09286109248644965	3.9407355724955107
>100	0.08360407344012011	9.964741767366013
>500	0.007828096188989584	3.1951894751543564
>1k	0.0012524953902383336	1.0916164210130186
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2058	0.19608646479352324	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1545	0.1472077687589861	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1544	0.14711248864975698	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1476	0.14063344122217702	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1302	0.12405470221631062	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1222	0.11643229347798123	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1173	0.1117635681257545	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	1095	0.10433171960588336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	9.528010922911722E-5	0.0	0.0	9.528010922911722E-5	0.0
16	9.528010922911722E-5	0.0	0.0	3.8112043691646886E-4	0.0
17	1.9056021845823443E-4	0.0	0.0	0.0010480812015202894	0.0
18	1.9056021845823443E-4	0.0	0.0	0.0014292016384367583	0.0
19	1.9056021845823443E-4	0.0	0.0	0.0018103220753532272	0.0
20	1.9056021845823443E-4	0.0	0.0	0.0022867226214988135	0.0
21	1.9056021845823443E-4	0.0	0.0	0.0027631231676443994	0.0
22	1.9056021845823443E-4	0.0	0.0	0.003334803823019103	0.0
23	1.9056021845823443E-4	0.0	0.0	0.005240406007601447	0.0
24	1.9056021845823443E-4	0.0	0.0	0.008289369502933198	0.0
25	1.9056021845823443E-4	0.0	0.0	0.01029025179674466	0.0
26	1.9056021845823443E-4	0.0	0.0	0.012481694309014356	0.0
27	1.9056021845823443E-4	0.0	0.0	0.020389943375031087	0.0
28	1.9056021845823443E-4	0.0	0.0	0.040303486203916584	0.0
29	1.9056021845823443E-4	0.0	0.0	0.07431848519871143	0.0
30	1.9056021845823443E-4	0.0	0.0	0.1216726994855827	0.0
31	2.858403276873517E-4	0.0	0.0	0.23943891449277158	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGA	30	3.5992666E-4	30.833334	2
AGTATCG	35	8.8688784E-4	26.428572	9
ATCGGAT	35	8.8688784E-4	26.428572	12
TTCGTTC	100	5.347829E-10	22.2	29
CGTTTAG	50	2.701885E-4	22.2	26
TTTCGTT	115	1.3460522E-10	20.913044	28
GGTATCA	705	0.0	20.468084	1
CTTATAC	345	0.0	20.376812	37
TATCGCG	75	9.264515E-6	19.733334	7
CAATACG	75	9.264515E-6	19.733334	4
TGACCGG	85	1.2450964E-6	19.588236	10
TCTAGCG	110	3.8506187E-8	18.5	28
ACCTAGG	510	0.0	18.137257	1
CGGACCA	145	1.546141E-10	17.862068	9
TTAGAGT	95	3.6057827E-6	17.526316	4
CGATTTT	95	3.6057827E-6	17.526316	21
CCGATTT	85	2.7227507E-5	17.411764	20
GCGAAAG	160	3.45608E-11	17.34375	18
ACGCTAT	75	2.067051E-4	17.266666	10
TCGCGCG	75	2.067051E-4	17.266666	9
>>END_MODULE