FastQCFastQC Report
Fri 10 Feb 2017
ERR1630317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312042
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20240.6486306330558066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19340.6197883618230879No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13150.4214176296780562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.28617942456464196No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG7020.22496971561520562No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5420.17369456675703912No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT4480.14357041680286628No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT4320.13844290191704964No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC4080.13075162958832465No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT4020.1288288115061434No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG4010.12850834182577986No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG3960.12690599342396217No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA3640.11665096365232885No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA3570.11440767588978408No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC3380.1083187519628768No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG3350.10735734292178617No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA3270.10479358547887785No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG3220.10319123707706014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA250.00549028329.610
CGCGTAA1000.027.75000210
GGTATCA5150.025.8640771
GCGTAAC1150.025.7391311
AGCGTCA751.36551535E-824.6666663
GTCGCGT1002.0008883E-1124.0500018
GGTCGCG1002.0008883E-1124.0500017
AAGTTGC551.8969042E-523.5454547
TAGGACA400.001928052223.1254
TCGCGTA1054.0017767E-1122.9047649
CGTAACT1300.022.7692312
TTAATAC502.6959923E-422.23
GTAACTA1250.022.19999913
GGGTCGC1107.2759576E-1121.8636366
TCGTTCG957.0940587E-921.42105337
GCAGCGT909.422911E-820.5555551
AGTCTCG1351.8189894E-1220.55555333
TTGGATC656.881553E-519.92307734
GTCTCGT1303.274181E-1119.92307734
TCTCGTT1303.274181E-1119.92307735
Produced by FastQC (version 0.11.5)